Source 1primary paper2025MED
Toolkit/single-construct optogenetic talin
single-construct optogenetic talin
Also known as: single-construct optodimerizable talin
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Single-construct optogenetic talin is an engineered light-responsive talin system in which pdDronpa1.2 enables light-inducible C-terminal talin homodimerization. In the cited study, this induced talin recruitment to adhesion sites, promoted adhesion formation, engaged actin retrograde flow, and activated downstream mechanosignaling.
Usefulness & Problems
Why this is useful
This tool provides optical control over talin-dependent localization and mechanotransduction within a single genetic construct. It is specifically presented as a way to avoid stoichiometric balance and multiplexing limitations associated with prior dual-construct heterodimerization systems, while remaining compatible with quantitative actin dynamics imaging and super-resolution single-molecule tracking.
Source:
The paper develops a single-construct optogenetic talin that uses pdDronpa1.2 for light-inducible C-terminal homodimerization.
Problem solved
The tool addresses the difficulty of controlling talin-mediated adhesion assembly and mechanosignaling with optogenetic systems that require two separately expressed components. By consolidating the light-responsive function into a single construct, it is intended to reduce stoichiometric imbalance and facilitate multiplexed imaging experiments.
Source:
The paper develops a single-construct optogenetic talin that uses pdDronpa1.2 for light-inducible C-terminal homodimerization.
Problem links
Need conditional control of signaling activity
DerivedSingle-construct optogenetic talin is an engineered light-responsive talin system that uses pdDronpa1.2 to drive light-inducible C-terminal talin homodimerization. In the cited study, this artificial homodimerization was sufficient to promote talin recruitment to adhesion sites, adhesion formation, actin retrograde flow engagement, and downstream mechanosignaling.
Need inducible protein relocalization or recruitment
DerivedSingle-construct optogenetic talin is an engineered light-responsive talin system that uses pdDronpa1.2 to drive light-inducible C-terminal talin homodimerization. In the cited study, this artificial homodimerization was sufficient to promote talin recruitment to adhesion sites, adhesion formation, actin retrograde flow engagement, and downstream mechanosignaling.
Need precise spatiotemporal control with light input
DerivedSingle-construct optogenetic talin is an engineered light-responsive talin system that uses pdDronpa1.2 to drive light-inducible C-terminal talin homodimerization. In the cited study, this artificial homodimerization was sufficient to promote talin recruitment to adhesion sites, adhesion formation, actin retrograde flow engagement, and downstream mechanosignaling.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
Heterodimerizationlight-induced homodimerizationlight-induced homodimerizationmechanosignaling activationmechanosignaling activationoptogenetic control of protein localizationoptogenetic control of protein localizationTechniques
No technique tags yet.
Target processes
localizationsignalingInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: multi component delivery burdenimplementation constraint: spectral hardware requirementmechanism: light-inducible C-terminal homodimerizationoperating role: regulatoroptogenetic: Truesingle construct: Trueswitch architecture: multi componentswitch architecture: recruitmenttarget protein: talin
The construct uses pdDronpa1.2 and is designed as a single-construct optodimerizable talin in which light induces C-terminal talin homodimerization. The supplied evidence supports use in imaging-compatible experiments, but it does not provide detailed construct architecture, expression conditions, or illumination wavelengths.
The available evidence is limited to a single cited study, so independent replication is not established. The provided evidence does not specify illumination parameters, cell types, dynamic range, reversibility, or performance relative to alternative optogenetic talin designs.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The single-construct optogenetic talin is intended to overcome stoichiometric balance and multiplexing limitations of prior dual-construct heterodimerization approaches.
The single-construct optogenetic talin is intended to overcome stoichiometric balance and multiplexing limitations of prior dual-construct heterodimerization approaches.
The single-construct optogenetic talin is intended to overcome stoichiometric balance and multiplexing limitations of prior dual-construct heterodimerization approaches.
The single-construct optogenetic talin is intended to overcome stoichiometric balance and multiplexing limitations of prior dual-construct heterodimerization approaches.
The single-construct optogenetic talin is intended to overcome stoichiometric balance and multiplexing limitations of prior dual-construct heterodimerization approaches.
The single-construct optogenetic talin is intended to overcome stoichiometric balance and multiplexing limitations of prior dual-construct heterodimerization approaches.
The single-construct optogenetic talin is intended to overcome stoichiometric balance and multiplexing limitations of prior dual-construct heterodimerization approaches.
The single-construct optogenetic talin is intended to overcome stoichiometric balance and multiplexing limitations of prior dual-construct heterodimerization approaches.
The single-construct optogenetic talin is intended to overcome stoichiometric balance and multiplexing limitations of prior dual-construct heterodimerization approaches.
The single-construct optogenetic talin is intended to overcome stoichiometric balance and multiplexing limitations of prior dual-construct heterodimerization approaches.
The single-construct optogenetic talin is intended to overcome stoichiometric balance and multiplexing limitations of prior dual-construct heterodimerization approaches.
The single-construct optogenetic talin is intended to overcome stoichiometric balance and multiplexing limitations of prior dual-construct heterodimerization approaches.
The single-construct optogenetic talin is intended to overcome stoichiometric balance and multiplexing limitations of prior dual-construct heterodimerization approaches.
The single-construct optogenetic talin is intended to overcome stoichiometric balance and multiplexing limitations of prior dual-construct heterodimerization approaches.
The single-construct optogenetic talin is intended to overcome stoichiometric balance and multiplexing limitations of prior dual-construct heterodimerization approaches.
The single-construct optogenetic talin is intended to overcome stoichiometric balance and multiplexing limitations of prior dual-construct heterodimerization approaches.
The single-construct optogenetic talin is intended to overcome stoichiometric balance and multiplexing limitations of prior dual-construct heterodimerization approaches.
The single-construct optogenetic talin is intended to overcome stoichiometric balance and multiplexing limitations of prior dual-construct heterodimerization approaches.
The single-construct optogenetic talin is intended to overcome stoichiometric balance and multiplexing limitations of prior dual-construct heterodimerization approaches.
The single-construct optogenetic talin is intended to overcome stoichiometric balance and multiplexing limitations of prior dual-construct heterodimerization approaches.
The single-construct optogenetic talin is intended to overcome stoichiometric balance and multiplexing limitations of prior dual-construct heterodimerization approaches.
The single-construct optogenetic talin is intended to overcome stoichiometric balance and multiplexing limitations of prior dual-construct heterodimerization approaches.
The single-construct optogenetic talin is intended to overcome stoichiometric balance and multiplexing limitations of prior dual-construct heterodimerization approaches.
The single-construct optodimerizable talin can be multiplexed with quantitative actin dynamics imaging or super-resolution single-molecule tracking.
The single-construct optodimerizable talin can be multiplexed with quantitative actin dynamics imaging or super-resolution single-molecule tracking.
The single-construct optodimerizable talin can be multiplexed with quantitative actin dynamics imaging or super-resolution single-molecule tracking.
The single-construct optodimerizable talin can be multiplexed with quantitative actin dynamics imaging or super-resolution single-molecule tracking.
The single-construct optodimerizable talin can be multiplexed with quantitative actin dynamics imaging or super-resolution single-molecule tracking.
The single-construct optodimerizable talin can be multiplexed with quantitative actin dynamics imaging or super-resolution single-molecule tracking.
The single-construct optodimerizable talin can be multiplexed with quantitative actin dynamics imaging or super-resolution single-molecule tracking.
The single-construct optodimerizable talin can be multiplexed with quantitative actin dynamics imaging or super-resolution single-molecule tracking.
The single-construct optodimerizable talin can be multiplexed with quantitative actin dynamics imaging or super-resolution single-molecule tracking.
The single-construct optodimerizable talin can be multiplexed with quantitative actin dynamics imaging or super-resolution single-molecule tracking.
The single-construct optodimerizable talin can be multiplexed with quantitative actin dynamics imaging or super-resolution single-molecule tracking.
The single-construct optodimerizable talin can be multiplexed with quantitative actin dynamics imaging or super-resolution single-molecule tracking.
The single-construct optodimerizable talin can be multiplexed with quantitative actin dynamics imaging or super-resolution single-molecule tracking.
The single-construct optodimerizable talin can be multiplexed with quantitative actin dynamics imaging or super-resolution single-molecule tracking.
The single-construct optodimerizable talin can be multiplexed with quantitative actin dynamics imaging or super-resolution single-molecule tracking.
The single-construct optodimerizable talin can be multiplexed with quantitative actin dynamics imaging or super-resolution single-molecule tracking.
The single-construct optodimerizable talin can be multiplexed with quantitative actin dynamics imaging or super-resolution single-molecule tracking.
The single-construct optodimerizable talin can be multiplexed with quantitative actin dynamics imaging or super-resolution single-molecule tracking.
The single-construct optodimerizable talin can be multiplexed with quantitative actin dynamics imaging or super-resolution single-molecule tracking.
The single-construct optodimerizable talin can be multiplexed with quantitative actin dynamics imaging or super-resolution single-molecule tracking.
The single-construct optodimerizable talin can be multiplexed with quantitative actin dynamics imaging or super-resolution single-molecule tracking.
The single-construct optodimerizable talin can be multiplexed with quantitative actin dynamics imaging or super-resolution single-molecule tracking.
The single-construct optodimerizable talin can be multiplexed with quantitative actin dynamics imaging or super-resolution single-molecule tracking.
Artificial light-induced homodimerization of talin is sufficient to promote talin recruitment to adhesion sites, adhesion formation, actin retrograde flow engagement, and downstream mechanosignaling.
Artificial light-induced homodimerization of talin is sufficient to promote talin recruitment to adhesion sites, adhesion formation, actin retrograde flow engagement, and downstream mechanosignaling.
Artificial light-induced homodimerization of talin is sufficient to promote talin recruitment to adhesion sites, adhesion formation, actin retrograde flow engagement, and downstream mechanosignaling.
Artificial light-induced homodimerization of talin is sufficient to promote talin recruitment to adhesion sites, adhesion formation, actin retrograde flow engagement, and downstream mechanosignaling.
Artificial light-induced homodimerization of talin is sufficient to promote talin recruitment to adhesion sites, adhesion formation, actin retrograde flow engagement, and downstream mechanosignaling.
Artificial light-induced homodimerization of talin is sufficient to promote talin recruitment to adhesion sites, adhesion formation, actin retrograde flow engagement, and downstream mechanosignaling.
Artificial light-induced homodimerization of talin is sufficient to promote talin recruitment to adhesion sites, adhesion formation, actin retrograde flow engagement, and downstream mechanosignaling.
Artificial light-induced homodimerization of talin is sufficient to promote talin recruitment to adhesion sites, adhesion formation, actin retrograde flow engagement, and downstream mechanosignaling.
Artificial light-induced homodimerization of talin is sufficient to promote talin recruitment to adhesion sites, adhesion formation, actin retrograde flow engagement, and downstream mechanosignaling.
Artificial light-induced homodimerization of talin is sufficient to promote talin recruitment to adhesion sites, adhesion formation, actin retrograde flow engagement, and downstream mechanosignaling.
Artificial light-induced homodimerization of talin is sufficient to promote talin recruitment to adhesion sites, adhesion formation, actin retrograde flow engagement, and downstream mechanosignaling.
Artificial light-induced homodimerization of talin is sufficient to promote talin recruitment to adhesion sites, adhesion formation, actin retrograde flow engagement, and downstream mechanosignaling.
Artificial light-induced homodimerization of talin is sufficient to promote talin recruitment to adhesion sites, adhesion formation, actin retrograde flow engagement, and downstream mechanosignaling.
Artificial light-induced homodimerization of talin is sufficient to promote talin recruitment to adhesion sites, adhesion formation, actin retrograde flow engagement, and downstream mechanosignaling.
Artificial light-induced homodimerization of talin is sufficient to promote talin recruitment to adhesion sites, adhesion formation, actin retrograde flow engagement, and downstream mechanosignaling.
Artificial light-induced homodimerization of talin is sufficient to promote talin recruitment to adhesion sites, adhesion formation, actin retrograde flow engagement, and downstream mechanosignaling.
Artificial light-induced homodimerization of talin is sufficient to promote talin recruitment to adhesion sites, adhesion formation, actin retrograde flow engagement, and downstream mechanosignaling.
Artificial light-induced homodimerization of talin is sufficient to promote talin recruitment to adhesion sites, adhesion formation, actin retrograde flow engagement, and downstream mechanosignaling.
Artificial light-induced homodimerization of talin is sufficient to promote talin recruitment to adhesion sites, adhesion formation, actin retrograde flow engagement, and downstream mechanosignaling.
Artificial light-induced homodimerization of talin is sufficient to promote talin recruitment to adhesion sites, adhesion formation, actin retrograde flow engagement, and downstream mechanosignaling.
Artificial light-induced homodimerization of talin is sufficient to promote talin recruitment to adhesion sites, adhesion formation, actin retrograde flow engagement, and downstream mechanosignaling.
Artificial light-induced homodimerization of talin is sufficient to promote talin recruitment to adhesion sites, adhesion formation, actin retrograde flow engagement, and downstream mechanosignaling.
Artificial light-induced homodimerization of talin is sufficient to promote talin recruitment to adhesion sites, adhesion formation, actin retrograde flow engagement, and downstream mechanosignaling.
The paper develops a single-construct optogenetic talin that uses pdDronpa1.2 for light-inducible C-terminal homodimerization.
The paper develops a single-construct optogenetic talin that uses pdDronpa1.2 for light-inducible C-terminal homodimerization.
The paper develops a single-construct optogenetic talin that uses pdDronpa1.2 for light-inducible C-terminal homodimerization.
The paper develops a single-construct optogenetic talin that uses pdDronpa1.2 for light-inducible C-terminal homodimerization.
The paper develops a single-construct optogenetic talin that uses pdDronpa1.2 for light-inducible C-terminal homodimerization.
The paper develops a single-construct optogenetic talin that uses pdDronpa1.2 for light-inducible C-terminal homodimerization.
The paper develops a single-construct optogenetic talin that uses pdDronpa1.2 for light-inducible C-terminal homodimerization.
The paper develops a single-construct optogenetic talin that uses pdDronpa1.2 for light-inducible C-terminal homodimerization.
The paper develops a single-construct optogenetic talin that uses pdDronpa1.2 for light-inducible C-terminal homodimerization.
The paper develops a single-construct optogenetic talin that uses pdDronpa1.2 for light-inducible C-terminal homodimerization.
The paper develops a single-construct optogenetic talin that uses pdDronpa1.2 for light-inducible C-terminal homodimerization.
The paper develops a single-construct optogenetic talin that uses pdDronpa1.2 for light-inducible C-terminal homodimerization.
The paper develops a single-construct optogenetic talin that uses pdDronpa1.2 for light-inducible C-terminal homodimerization.
The paper develops a single-construct optogenetic talin that uses pdDronpa1.2 for light-inducible C-terminal homodimerization.
The paper develops a single-construct optogenetic talin that uses pdDronpa1.2 for light-inducible C-terminal homodimerization.
The paper develops a single-construct optogenetic talin that uses pdDronpa1.2 for light-inducible C-terminal homodimerization.
The paper develops a single-construct optogenetic talin that uses pdDronpa1.2 for light-inducible C-terminal homodimerization.
The paper develops a single-construct optogenetic talin that uses pdDronpa1.2 for light-inducible C-terminal homodimerization.
The paper develops a single-construct optogenetic talin that uses pdDronpa1.2 for light-inducible C-terminal homodimerization.
The paper develops a single-construct optogenetic talin that uses pdDronpa1.2 for light-inducible C-terminal homodimerization.
The paper develops a single-construct optogenetic talin that uses pdDronpa1.2 for light-inducible C-terminal homodimerization.
The paper develops a single-construct optogenetic talin that uses pdDronpa1.2 for light-inducible C-terminal homodimerization.
The paper develops a single-construct optogenetic talin that uses pdDronpa1.2 for light-inducible C-terminal homodimerization.
Approval Evidence
1 source4 linked approval claimsfirst-pass slug single-construct-optogenetic-talin
we develop a single-construct optogenetic talin utilizing pdDronpa1.2 for light-inducible C-terminal homodimerization
Source:
comparative advantagesupports
The single-construct optogenetic talin is intended to overcome stoichiometric balance and multiplexing limitations of prior dual-construct heterodimerization approaches.
Source:
compatibilitysupports
The single-construct optodimerizable talin can be multiplexed with quantitative actin dynamics imaging or super-resolution single-molecule tracking.
Source:
mechanistic effectsupports
Artificial light-induced homodimerization of talin is sufficient to promote talin recruitment to adhesion sites, adhesion formation, actin retrograde flow engagement, and downstream mechanosignaling.
Source:
tool developmentsupports
The paper develops a single-construct optogenetic talin that uses pdDronpa1.2 for light-inducible C-terminal homodimerization.
Source:
Comparisons
Source-backed strengths
The cited study reports that light-induced talin homodimerization was sufficient to drive several downstream outputs: recruitment to adhesion sites, adhesion formation, coupling to actin retrograde flow, and mechanosignaling activation. A further practical strength is compatibility with quantitative actin dynamics imaging and super-resolution single-molecule tracking.
Source:
The single-construct optogenetic talin is intended to overcome stoichiometric balance and multiplexing limitations of prior dual-construct heterodimerization approaches.
Compared with fusion proteins with large N-terminal anchors
single-construct optogenetic talin and fusion proteins with large N-terminal anchors address a similar problem space because they share localization, signaling.
Shared frame: same top-level item type; shared target processes: localization, signaling; shared mechanisms: heterodimerization; same primary input modality: light
Compared with iLID/SspB
single-construct optogenetic talin and iLID/SspB address a similar problem space because they share localization, signaling.
Shared frame: same top-level item type; shared target processes: localization, signaling; shared mechanisms: heterodimerization; same primary input modality: light
Relative tradeoffs: appears more independently replicated; looks easier to implement in practice.
Compared with LOVpep/ePDZb
single-construct optogenetic talin and LOVpep/ePDZb address a similar problem space because they share localization, signaling.
Shared frame: same top-level item type; shared target processes: localization, signaling; shared mechanisms: heterodimerization; same primary input modality: light
Relative tradeoffs: appears more independently replicated; looks easier to implement in practice.
Ranked Citations
- 1.