Source 1primary paper2026MED
Toolkit/single-walled carbon nanotubes
single-walled carbon nanotubes
Also known as: SWCNTs
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Recent research has shown that plasmid DNA delivered by single-walled carbon nanotubes (SWCNTs) ... can diffuse through plant cell walls, enabling the transient expression of genetic material in plant tissues.
Usefulness & Problems
No literature-backed usefulness or problem-fit explainer has been materialized for this record yet.
Published Workflows
Objective: Test whether SWCNT- or CD-based plasmid delivery by leaf infiltration can enable reporter expression and CRISPR-Cas9 editing in cowpea, a legume described as recalcitrant for transformation.
Why it works: The abstract states that plasmid DNA delivered by SWCNTs and CDs can diffuse through plant cell walls, enabling transient expression of genetic material in plant tissues.
diffusion of plasmid-loaded nanocarriers through plant cell wallstransient expression of delivered genetic materialtargeted CRISPR-Cas9 editing at PDSleaf infiltrationreporter-gene readoutCRISPR-Cas9 vector delivery
Stages
- 1.Reporter-gene delivery and transient expression test(functional_characterization)
This stage tests whether the nanocarrier system can deliver plasmid DNA and produce observable reporter expression in cowpea leaves.
Selection: Ability of SWCNT- or CD-based plasmid delivery to express a target gene in cowpea leaves after infiltration.
- 2.CRISPR-Cas9 delivery and editing assessment(confirmatory_validation)
This stage tests whether the nanocarrier delivery approach extends beyond transient reporter expression to CRISPR-Cas9-mediated editing.
Selection: Whether infiltrated CRISPR-Cas9 vectors targeting PDS produce editing outcomes in cowpea leaves.
Steps
- 1.Infiltrate cowpea leaves with SWCNT- or CD-delivered GUS reporter plasmidnanocarrier delivery harness
Introduce a reporter plasmid into cowpea leaf tissue to test whether the delivery system can support expression.
The abstract presents reporter-gene expression testing as the initial investigation before CRISPR-Cas9 delivery assessment.
- 2.Assess temporary GUS expression near the infiltration site by blue-color observation
Determine whether delivered reporter plasmid is transiently expressed in the surrounding infiltrated area.
This provides the stated readout for whether the nanocarrier delivery system achieved local expression before moving to CRISPR-Cas9 editing assessment.
- 3.Infiltrate cowpea leaves with CRISPR-Cas9 vectors targeting PDS using SWCNTs or CDsnanocarrier delivery harness
Test whether the nanocarrier system can deliver genome-editing constructs into cowpea leaves.
The abstract presents this as a follow-on test after reporter-gene delivery, extending the system from expression to editing.
- 4.Assess PDS editing outcomes for multiplex editing and large deletions
Determine whether delivered CRISPR-Cas9 vectors produced target-gene knockout-associated editing outcomes.
This is the confirmatory readout for whether nanocarrier-mediated delivery supports functional genome editing beyond transient expression.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A delivery strategy grouped with the mechanism branch because it determines how a system is instantiated and deployed in context.
Mechanisms
diffusion through plant cell wallsnanocarrier-mediated plasmid deliverytransient transgene expressionTechniques
No technique tags yet.
Target processes
editingValidation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Single-walled carbon nanotubes and carbon dots delivered a GUS reporter plasmid into cowpea leaves near the infiltration site, producing temporary GUS expression.
Infiltration of CRISPR-Cas9 vectors targeting PDS in cowpea leaves resulted in multiplex editing and large deletions within the target gene.
SWCNT- and CD-mediated plasmid DNA delivery is presented as a way to overcome conventional DNA delivery challenges in cowpea and other recalcitrant plant species.
Approval Evidence
1 source3 linked approval claimsfirst-pass slug single-walled-carbon-nanotubes
Recent research has shown that plasmid DNA delivered by single-walled carbon nanotubes (SWCNTs) ... can diffuse through plant cell walls, enabling the transient expression of genetic material in plant tissues.
Source:
application resultsupports
Single-walled carbon nanotubes and carbon dots delivered a GUS reporter plasmid into cowpea leaves near the infiltration site, producing temporary GUS expression.
Source:
editing resultsupports
Infiltration of CRISPR-Cas9 vectors targeting PDS in cowpea leaves resulted in multiplex editing and large deletions within the target gene.
Source:
problem statementsupports
SWCNT- and CD-mediated plasmid DNA delivery is presented as a way to overcome conventional DNA delivery challenges in cowpea and other recalcitrant plant species.
Source:
Comparisons
No literature-backed comparison notes have been materialized for this record yet.
Ranked Citations
- 1.