Source 1primary paper2025Chemical Science
Toolkit/SiNx solid-state nanopore single-cell protein analysis approach
SiNx solid-state nanopore single-cell protein analysis approach
Also known as: SiNx solid-state nanopores for single-molecule protein analysis from single cells
Taxonomy: Mechanism Branch / Component. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The SiNx solid-state nanopore single-cell protein analysis approach combines glass nanopipette-based single-cell extraction with silicon nitride nanopore sensing for single-molecule protein analysis from complex cellular samples. In the cited Chemical Science study, this workflow identified the LOV2 protein and monitored its conformational changes directly from single-cell extracts.
Usefulness & Problems
Why this is useful
This approach is useful for probing proteins at the single-molecule level directly from individual cells rather than relying only on purified preparations. The reported ability to identify LOV2 and distinguish conformational behavior in single-cell extracts indicates utility for studying protein state in complex native-like cellular material.
Problem solved
It addresses the problem of measuring and identifying proteins from single cells using a solid-state nanopore platform despite the complexity of cellular samples. It also addresses the gap between purified-sample measurements and direct single-cell measurements, because the study reported significant differences between proteins measured from single cells and proteins obtained from purified samples.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Component: A low-level protein part used inside a larger architecture that realizes a mechanism.
Mechanisms
conformational state discriminationconformational state discriminationConformational Uncagingnanopore-based single-molecule sensingnanopore-based single-molecule sensingTechniques
No technique tags yet.
Target processes
No target processes tagged yet.
Implementation Constraints
analyte class: proteincofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: actuatorsingle cell: Truesingle molecule: True
The reported implementation couples glass nanopipette-based single-cell extraction to silicon nitride solid-state nanopore measurements. Beyond this workflow and the use of LOV2 as the demonstrated target, the supplied evidence does not provide construct design details, buffer conditions, pore fabrication parameters, or delivery requirements.
The supplied evidence describes one reported application centered on LOV2, so generality across other proteins, cell types, and assay conditions is not established here. The evidence also indicates that measurements from direct single-cell samples differ significantly from purified samples, which highlights biological relevance but also implies that purified-sample behavior may not transfer straightforwardly to this workflow.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Using glass nanopipette-based single-cell extraction together with SiNx nanopores, the authors identified LOV2 and monitored its conformational changes from single-cell extracts.
we performed ex situ single-cell protein analysis by directly extracting the contents of individual cells using glass nanopipette-based single-cell extraction and successfully identified and monitored the conformational changes of the LOV2 protein from single-cell extracts using SiN x nanopores
Using glass nanopipette-based single-cell extraction together with SiNx nanopores, the authors identified LOV2 and monitored its conformational changes from single-cell extracts.
we performed ex situ single-cell protein analysis by directly extracting the contents of individual cells using glass nanopipette-based single-cell extraction and successfully identified and monitored the conformational changes of the LOV2 protein from single-cell extracts using SiN x nanopores
Using glass nanopipette-based single-cell extraction together with SiNx nanopores, the authors identified LOV2 and monitored its conformational changes from single-cell extracts.
we performed ex situ single-cell protein analysis by directly extracting the contents of individual cells using glass nanopipette-based single-cell extraction and successfully identified and monitored the conformational changes of the LOV2 protein from single-cell extracts using SiN x nanopores
Using glass nanopipette-based single-cell extraction together with SiNx nanopores, the authors identified LOV2 and monitored its conformational changes from single-cell extracts.
we performed ex situ single-cell protein analysis by directly extracting the contents of individual cells using glass nanopipette-based single-cell extraction and successfully identified and monitored the conformational changes of the LOV2 protein from single-cell extracts using SiN x nanopores
Using glass nanopipette-based single-cell extraction together with SiNx nanopores, the authors identified LOV2 and monitored its conformational changes from single-cell extracts.
we performed ex situ single-cell protein analysis by directly extracting the contents of individual cells using glass nanopipette-based single-cell extraction and successfully identified and monitored the conformational changes of the LOV2 protein from single-cell extracts using SiN x nanopores
SiNx solid-state nanopores can be used for single-molecule protein analysis from complex cellular samples.
Here, we explored the potential of SiN x solid-state nanopores for single-molecule protein analysis from complex cellular samples.
SiNx solid-state nanopores can be used for single-molecule protein analysis from complex cellular samples.
Here, we explored the potential of SiN x solid-state nanopores for single-molecule protein analysis from complex cellular samples.
SiNx solid-state nanopores can be used for single-molecule protein analysis from complex cellular samples.
Here, we explored the potential of SiN x solid-state nanopores for single-molecule protein analysis from complex cellular samples.
SiNx solid-state nanopores can be used for single-molecule protein analysis from complex cellular samples.
Here, we explored the potential of SiN x solid-state nanopores for single-molecule protein analysis from complex cellular samples.
SiNx solid-state nanopores can be used for single-molecule protein analysis from complex cellular samples.
Here, we explored the potential of SiN x solid-state nanopores for single-molecule protein analysis from complex cellular samples.
Proteins measured directly from single cells differed significantly from proteins obtained from purified samples.
Our results reveal significant differences between proteins measured directly from single cells and those obtained from purified samples.
Proteins measured directly from single cells differed significantly from proteins obtained from purified samples.
Our results reveal significant differences between proteins measured directly from single cells and those obtained from purified samples.
Proteins measured directly from single cells differed significantly from proteins obtained from purified samples.
Our results reveal significant differences between proteins measured directly from single cells and those obtained from purified samples.
Proteins measured directly from single cells differed significantly from proteins obtained from purified samples.
Our results reveal significant differences between proteins measured directly from single cells and those obtained from purified samples.
Proteins measured directly from single cells differed significantly from proteins obtained from purified samples.
Our results reveal significant differences between proteins measured directly from single cells and those obtained from purified samples.
Approval Evidence
1 source3 linked approval claimsfirst-pass slug sinx-solid-state-nanopore-single-cell-protein-analysis-approach
Here, we explored the potential of SiN x solid-state nanopores for single-molecule protein analysis from complex cellular samples... successfully identified and monitored the conformational changes of the LOV2 protein from single-cell extracts using SiN x nanopores.
Source:
application resultsupports
Using glass nanopipette-based single-cell extraction together with SiNx nanopores, the authors identified LOV2 and monitored its conformational changes from single-cell extracts.
we performed ex situ single-cell protein analysis by directly extracting the contents of individual cells using glass nanopipette-based single-cell extraction and successfully identified and monitored the conformational changes of the LOV2 protein from single-cell extracts using SiN x nanopores
Source:
capabilitysupports
SiNx solid-state nanopores can be used for single-molecule protein analysis from complex cellular samples.
Here, we explored the potential of SiN x solid-state nanopores for single-molecule protein analysis from complex cellular samples.
Source:
comparisonsupports
Proteins measured directly from single cells differed significantly from proteins obtained from purified samples.
Our results reveal significant differences between proteins measured directly from single cells and those obtained from purified samples.
Source:
Comparisons
Source-backed strengths
The approach was demonstrated for single-molecule protein analysis from complex cellular samples using SiNx solid-state nanopores. A key validated result is direct identification of LOV2 and monitoring of its conformational changes from single-cell extracts, showing that the method can resolve protein state information in material obtained from individual cells.
Ranked Citations
- 1.