Source 1primary paper2023Clinical and Translational Medicine
Toolkit/Split-Cas9-based targeted gene editing
Split-Cas9-based targeted gene editing
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Split-Cas9-based targeted gene editing is a gene-editing component used in an optogenetically coordinated platform with nanobody-mediated proteolysis-targeting chimeras. In the reported application, it was used to regulate Survivin as part of a multi-level strategy to control cancer cell fate.
Usefulness & Problems
Why this is useful
This approach is useful for combining gene editing with light-coordinated regulation in cancer-cell systems. The cited study frames coordinated and combinable technology platforms as advantageous for controllability and targeting during regulation of key intracellular factors.
Source:
the coordinated regulatory strategies and combinable technology platforms offer clear advantages in controllability and targeting, as well as an excellent reference value and universal applicability in controlling the fate of cancer cells through multi-level regulation of key intracellular factors.
Problem solved
It addresses the problem of achieving multi-level regulation of an intracellular cancer-associated factor, specifically Survivin, to influence cancer cell fate. The evidence supports this application context but does not provide further mechanistic or performance detail for the split-Cas9 module alone.
Source:
the coordinated regulatory strategies and combinable technology platforms offer clear advantages in controllability and targeting, as well as an excellent reference value and universal applicability in controlling the fate of cancer cells through multi-level regulation of key intracellular factors.
Problem links
Need conditional protein clearance
DerivedSplit-Cas9-based targeted gene editing is an optogenetically coordinated gene-editing approach used together with nanobody-mediated proteolysis-targeting chimeras to regulate Survivin in cancer cells. The reported application is multi-level control of intracellular factors to influence cancer cell fate.
Need controllable genome or transcript editing
DerivedSplit-Cas9-based targeted gene editing is an optogenetically coordinated gene-editing approach used together with nanobody-mediated proteolysis-targeting chimeras to regulate Survivin in cancer cells. The reported application is multi-level control of intracellular factors to influence cancer cell fate.
Need precise spatiotemporal control with light input
DerivedSplit-Cas9-based targeted gene editing is an optogenetically coordinated gene-editing approach used together with nanobody-mediated proteolysis-targeting chimeras to regulate Survivin in cancer cells. The reported application is multi-level control of intracellular factors to influence cancer cell fate.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
Degradationgene editinggene editingoptogenetic regulationoptogenetic regulationprotein degradationprotein degradationTechniques
No technique tags yet.
Target processes
degradationeditingInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: builderswitch architecture: split
The available evidence indicates that the system is split-Cas9-based, light-coordinated, and combined with nanobody-mediated proteolysis-targeting chimeras in a Survivin-regulation context. No construct design details, delivery method, expression system, cofactor requirement, or illumination parameters are provided in the supplied evidence.
The supplied evidence does not describe the split architecture, Cas9 variant, light-responsive module, editing efficiency, specificity, or experimental performance metrics. Independent validation and breadth across targets, cell types, or organisms are not established from the provided material.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Coordinated regulatory strategies and combinable technology platforms offer advantages in controllability and targeting for controlling the fate of cancer cells through multi-level regulation of key intracellular factors.
the coordinated regulatory strategies and combinable technology platforms offer clear advantages in controllability and targeting, as well as an excellent reference value and universal applicability in controlling the fate of cancer cells through multi-level regulation of key intracellular factors.
Coordinated regulatory strategies and combinable technology platforms offer advantages in controllability and targeting for controlling the fate of cancer cells through multi-level regulation of key intracellular factors.
the coordinated regulatory strategies and combinable technology platforms offer clear advantages in controllability and targeting, as well as an excellent reference value and universal applicability in controlling the fate of cancer cells through multi-level regulation of key intracellular factors.
Coordinated regulatory strategies and combinable technology platforms offer advantages in controllability and targeting for controlling the fate of cancer cells through multi-level regulation of key intracellular factors.
the coordinated regulatory strategies and combinable technology platforms offer clear advantages in controllability and targeting, as well as an excellent reference value and universal applicability in controlling the fate of cancer cells through multi-level regulation of key intracellular factors.
Coordinated regulatory strategies and combinable technology platforms offer advantages in controllability and targeting for controlling the fate of cancer cells through multi-level regulation of key intracellular factors.
the coordinated regulatory strategies and combinable technology platforms offer clear advantages in controllability and targeting, as well as an excellent reference value and universal applicability in controlling the fate of cancer cells through multi-level regulation of key intracellular factors.
Coordinated regulatory strategies and combinable technology platforms offer advantages in controllability and targeting for controlling the fate of cancer cells through multi-level regulation of key intracellular factors.
the coordinated regulatory strategies and combinable technology platforms offer clear advantages in controllability and targeting, as well as an excellent reference value and universal applicability in controlling the fate of cancer cells through multi-level regulation of key intracellular factors.
Coordinated regulatory strategies and combinable technology platforms offer advantages in controllability and targeting for controlling the fate of cancer cells through multi-level regulation of key intracellular factors.
the coordinated regulatory strategies and combinable technology platforms offer clear advantages in controllability and targeting, as well as an excellent reference value and universal applicability in controlling the fate of cancer cells through multi-level regulation of key intracellular factors.
Coordinated regulatory strategies and combinable technology platforms offer advantages in controllability and targeting for controlling the fate of cancer cells through multi-level regulation of key intracellular factors.
the coordinated regulatory strategies and combinable technology platforms offer clear advantages in controllability and targeting, as well as an excellent reference value and universal applicability in controlling the fate of cancer cells through multi-level regulation of key intracellular factors.
Coordinated regulatory strategies and combinable technology platforms offer advantages in controllability and targeting for controlling the fate of cancer cells through multi-level regulation of key intracellular factors.
the coordinated regulatory strategies and combinable technology platforms offer clear advantages in controllability and targeting, as well as an excellent reference value and universal applicability in controlling the fate of cancer cells through multi-level regulation of key intracellular factors.
Coordinated regulatory strategies and combinable technology platforms offer advantages in controllability and targeting for controlling the fate of cancer cells through multi-level regulation of key intracellular factors.
the coordinated regulatory strategies and combinable technology platforms offer clear advantages in controllability and targeting, as well as an excellent reference value and universal applicability in controlling the fate of cancer cells through multi-level regulation of key intracellular factors.
Coordinated regulatory strategies and combinable technology platforms offer advantages in controllability and targeting for controlling the fate of cancer cells through multi-level regulation of key intracellular factors.
the coordinated regulatory strategies and combinable technology platforms offer clear advantages in controllability and targeting, as well as an excellent reference value and universal applicability in controlling the fate of cancer cells through multi-level regulation of key intracellular factors.
Coordinated regulatory strategies and combinable technology platforms offer advantages in controllability and targeting for controlling the fate of cancer cells through multi-level regulation of key intracellular factors.
the coordinated regulatory strategies and combinable technology platforms offer clear advantages in controllability and targeting, as well as an excellent reference value and universal applicability in controlling the fate of cancer cells through multi-level regulation of key intracellular factors.
Coordinated regulatory strategies and combinable technology platforms offer advantages in controllability and targeting for controlling the fate of cancer cells through multi-level regulation of key intracellular factors.
the coordinated regulatory strategies and combinable technology platforms offer clear advantages in controllability and targeting, as well as an excellent reference value and universal applicability in controlling the fate of cancer cells through multi-level regulation of key intracellular factors.
Coordinated regulatory strategies and combinable technology platforms offer advantages in controllability and targeting for controlling the fate of cancer cells through multi-level regulation of key intracellular factors.
the coordinated regulatory strategies and combinable technology platforms offer clear advantages in controllability and targeting, as well as an excellent reference value and universal applicability in controlling the fate of cancer cells through multi-level regulation of key intracellular factors.
Coordinated regulatory strategies and combinable technology platforms offer advantages in controllability and targeting for controlling the fate of cancer cells through multi-level regulation of key intracellular factors.
the coordinated regulatory strategies and combinable technology platforms offer clear advantages in controllability and targeting, as well as an excellent reference value and universal applicability in controlling the fate of cancer cells through multi-level regulation of key intracellular factors.
Coordinated regulatory strategies and combinable technology platforms offer advantages in controllability and targeting for controlling the fate of cancer cells through multi-level regulation of key intracellular factors.
the coordinated regulatory strategies and combinable technology platforms offer clear advantages in controllability and targeting, as well as an excellent reference value and universal applicability in controlling the fate of cancer cells through multi-level regulation of key intracellular factors.
Coordinated regulatory strategies and combinable technology platforms offer advantages in controllability and targeting for controlling the fate of cancer cells through multi-level regulation of key intracellular factors.
the coordinated regulatory strategies and combinable technology platforms offer clear advantages in controllability and targeting, as well as an excellent reference value and universal applicability in controlling the fate of cancer cells through multi-level regulation of key intracellular factors.
Coordinated regulatory strategies and combinable technology platforms offer advantages in controllability and targeting for controlling the fate of cancer cells through multi-level regulation of key intracellular factors.
the coordinated regulatory strategies and combinable technology platforms offer clear advantages in controllability and targeting, as well as an excellent reference value and universal applicability in controlling the fate of cancer cells through multi-level regulation of key intracellular factors.
Split-Cas9-based targeted gene editing and nanobody-mediated proteolysis-targeting chimeras are used for optogenetically coordinated regulation of Survivin to control the fate of cancer cells.
Split‐Cas9‐based targeted gene editing and nanobody‐mediated proteolysis‐targeting chimeras optogenetically coordinated regulation of Survivin to control the fate of cancer cells
Split-Cas9-based targeted gene editing and nanobody-mediated proteolysis-targeting chimeras are used for optogenetically coordinated regulation of Survivin to control the fate of cancer cells.
Split‐Cas9‐based targeted gene editing and nanobody‐mediated proteolysis‐targeting chimeras optogenetically coordinated regulation of Survivin to control the fate of cancer cells
Split-Cas9-based targeted gene editing and nanobody-mediated proteolysis-targeting chimeras are used for optogenetically coordinated regulation of Survivin to control the fate of cancer cells.
Split‐Cas9‐based targeted gene editing and nanobody‐mediated proteolysis‐targeting chimeras optogenetically coordinated regulation of Survivin to control the fate of cancer cells
Split-Cas9-based targeted gene editing and nanobody-mediated proteolysis-targeting chimeras are used for optogenetically coordinated regulation of Survivin to control the fate of cancer cells.
Split‐Cas9‐based targeted gene editing and nanobody‐mediated proteolysis‐targeting chimeras optogenetically coordinated regulation of Survivin to control the fate of cancer cells
Split-Cas9-based targeted gene editing and nanobody-mediated proteolysis-targeting chimeras are used for optogenetically coordinated regulation of Survivin to control the fate of cancer cells.
Split‐Cas9‐based targeted gene editing and nanobody‐mediated proteolysis‐targeting chimeras optogenetically coordinated regulation of Survivin to control the fate of cancer cells
Split-Cas9-based targeted gene editing and nanobody-mediated proteolysis-targeting chimeras are used for optogenetically coordinated regulation of Survivin to control the fate of cancer cells.
Split‐Cas9‐based targeted gene editing and nanobody‐mediated proteolysis‐targeting chimeras optogenetically coordinated regulation of Survivin to control the fate of cancer cells
Split-Cas9-based targeted gene editing and nanobody-mediated proteolysis-targeting chimeras are used for optogenetically coordinated regulation of Survivin to control the fate of cancer cells.
Split‐Cas9‐based targeted gene editing and nanobody‐mediated proteolysis‐targeting chimeras optogenetically coordinated regulation of Survivin to control the fate of cancer cells
Split-Cas9-based targeted gene editing and nanobody-mediated proteolysis-targeting chimeras are used for optogenetically coordinated regulation of Survivin to control the fate of cancer cells.
Split‐Cas9‐based targeted gene editing and nanobody‐mediated proteolysis‐targeting chimeras optogenetically coordinated regulation of Survivin to control the fate of cancer cells
Split-Cas9-based targeted gene editing and nanobody-mediated proteolysis-targeting chimeras are used for optogenetically coordinated regulation of Survivin to control the fate of cancer cells.
Split‐Cas9‐based targeted gene editing and nanobody‐mediated proteolysis‐targeting chimeras optogenetically coordinated regulation of Survivin to control the fate of cancer cells
Split-Cas9-based targeted gene editing and nanobody-mediated proteolysis-targeting chimeras are used for optogenetically coordinated regulation of Survivin to control the fate of cancer cells.
Split‐Cas9‐based targeted gene editing and nanobody‐mediated proteolysis‐targeting chimeras optogenetically coordinated regulation of Survivin to control the fate of cancer cells
Split-Cas9-based targeted gene editing and nanobody-mediated proteolysis-targeting chimeras are used for optogenetically coordinated regulation of Survivin to control the fate of cancer cells.
Split‐Cas9‐based targeted gene editing and nanobody‐mediated proteolysis‐targeting chimeras optogenetically coordinated regulation of Survivin to control the fate of cancer cells
Split-Cas9-based targeted gene editing and nanobody-mediated proteolysis-targeting chimeras are used for optogenetically coordinated regulation of Survivin to control the fate of cancer cells.
Split‐Cas9‐based targeted gene editing and nanobody‐mediated proteolysis‐targeting chimeras optogenetically coordinated regulation of Survivin to control the fate of cancer cells
Split-Cas9-based targeted gene editing and nanobody-mediated proteolysis-targeting chimeras are used for optogenetically coordinated regulation of Survivin to control the fate of cancer cells.
Split‐Cas9‐based targeted gene editing and nanobody‐mediated proteolysis‐targeting chimeras optogenetically coordinated regulation of Survivin to control the fate of cancer cells
Split-Cas9-based targeted gene editing and nanobody-mediated proteolysis-targeting chimeras are used for optogenetically coordinated regulation of Survivin to control the fate of cancer cells.
Split‐Cas9‐based targeted gene editing and nanobody‐mediated proteolysis‐targeting chimeras optogenetically coordinated regulation of Survivin to control the fate of cancer cells
Split-Cas9-based targeted gene editing and nanobody-mediated proteolysis-targeting chimeras are used for optogenetically coordinated regulation of Survivin to control the fate of cancer cells.
Split‐Cas9‐based targeted gene editing and nanobody‐mediated proteolysis‐targeting chimeras optogenetically coordinated regulation of Survivin to control the fate of cancer cells
Split-Cas9-based targeted gene editing and nanobody-mediated proteolysis-targeting chimeras are used for optogenetically coordinated regulation of Survivin to control the fate of cancer cells.
Split‐Cas9‐based targeted gene editing and nanobody‐mediated proteolysis‐targeting chimeras optogenetically coordinated regulation of Survivin to control the fate of cancer cells
Split-Cas9-based targeted gene editing and nanobody-mediated proteolysis-targeting chimeras are used for optogenetically coordinated regulation of Survivin to control the fate of cancer cells.
Split‐Cas9‐based targeted gene editing and nanobody‐mediated proteolysis‐targeting chimeras optogenetically coordinated regulation of Survivin to control the fate of cancer cells
Approval Evidence
1 source2 linked approval claimsfirst-pass slug split-cas9-based-targeted-gene-editing
Split‐Cas9‐based targeted gene editing
Source:
application scopesupports
Coordinated regulatory strategies and combinable technology platforms offer advantages in controllability and targeting for controlling the fate of cancer cells through multi-level regulation of key intracellular factors.
the coordinated regulatory strategies and combinable technology platforms offer clear advantages in controllability and targeting, as well as an excellent reference value and universal applicability in controlling the fate of cancer cells through multi-level regulation of key intracellular factors.
Source:
target regulationsupports
Split-Cas9-based targeted gene editing and nanobody-mediated proteolysis-targeting chimeras are used for optogenetically coordinated regulation of Survivin to control the fate of cancer cells.
Split‐Cas9‐based targeted gene editing and nanobody‐mediated proteolysis‐targeting chimeras optogenetically coordinated regulation of Survivin to control the fate of cancer cells
Source:
Comparisons
Source-backed strengths
The reported strength is its use within a coordinated regulatory strategy that combines targeted gene editing with nanobody-mediated protein degradation under optogenetic control. The source specifically claims advantages in controllability and targeting for regulating cancer cell fate.
Compared with CRISPRoff
Split-Cas9-based targeted gene editing and CRISPRoff address a similar problem space because they share degradation, editing.
Shared frame: same top-level item type; shared target processes: degradation, editing; shared mechanisms: degradation; same primary input modality: light
Compared with light-engineering technology
Split-Cas9-based targeted gene editing and light-engineering technology address a similar problem space because they share degradation.
Shared frame: same top-level item type; shared target processes: degradation; shared mechanisms: degradation, protein degradation; same primary input modality: light
Compared with nanobody-mediated proteolysis-targeting chimeras
Split-Cas9-based targeted gene editing and nanobody-mediated proteolysis-targeting chimeras address a similar problem space because they share degradation, editing.
Shared frame: same top-level item type; shared target processes: degradation, editing; shared mechanisms: degradation, optogenetic regulation; same primary input modality: light
Ranked Citations
- 1.