Source 1review2016ACS Chemical Biology
Toolkit/split Cas9 logic gate
split Cas9 logic gate
Also known as: split Cas9, split-Cas9 constructs, split-Cas9 logic gate
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The split Cas9 logic gate is a synthetic multi-component circuit that uses split Cas9 halves to sense biological events through conditional reconstitution of Cas9 activity. In the reported 2023 implementation, self-assembling inteins reconstituted split Cas9 and enabled reporter activation only when specified transcriptional or cell-state inputs were present.
Usefulness & Problems
Why this is useful
This tool is useful for converting combinations of endogenous cellular features into a discrete reporter output. Reported applications included sensing cell-cell fusion, epithelial cancer origin, epithelial-to-mesenchymal transition, and the combination of epithelial origin with EMT status.
Source:
First, we positioned the complementing halves of split Cas9 under different promoters-one specific to cancer cells of epithelial origin (p hCEA) and the other a universal promoter... Consequently, only cancer cells with an epithelial origin activated the reporter, exhibiting green fluorescence.
Source:
we designed a logic gate where one half of Cas9 is expressed under the p hCEA, while the other is activated by TWIST1. The results showed that cells undergoing EMT effectively activated the reporter.
Source:
we combined the two inputs (epithelial origin and EMT) to create a new logic gate, where only cancer epithelial cells undergoing EMT activated the reporter
Source:
we applied the split-Cas9 logic gate as a sensor of cell-cell fusion, both in induced and naturally occurring scenarios. Each cell type expressed one half of split Cas9, and the induction of fusion resulted in the appearance of multinucleated syncytia and the fluorescent reporter.
Problem solved
It addresses the problem of detecting complex cellular events that are defined by multiple biological inputs rather than a single marker. The design enables logic-gated Cas9 activity so that reporter activation occurs only when the required input conditions are simultaneously satisfied.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
Heterodimerizationlogic-gated transcriptional input integrationprotein splicing-mediated reconstitutionsplit-protein complementationTechniques
Computational DesignTarget processes
editingrecombinationInput: Light
Implementation Constraints
The implementation used split Cas9 halves reconstituted by self-assembling inteins. Reported construct designs placed different split Cas9 halves under distinct promoters or input-responsive regulatory programs, including p hCEA, TWIST1-linked activation, an epithelial cancer-specific promoter, and a universal promoter, with output measured by reporter fluorescence.
The supplied evidence describes reporter-based sensing applications but does not provide quantitative performance metrics such as dynamic range, background activity, sensitivity, or timing. Evidence is drawn from a single 2023 study, and the provided material does not document independent replication or broader validation beyond the reported cellular event-detection contexts.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Observations
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c2 during normalization. A split Cas9 gate with one half under an epithelial cancer-specific promoter and the other under a universal promoter activated the reporter only in cancer cells with epithelial origin. Derived from claim c2. Quoted text: First, we positioned the complementing halves of split Cas9 under different promoters-one specific to cancer cells of epithelial origin (p hCEA) and the other a universal promoter... Consequently, only cancer cells with an epithelial origin activated the reporter, exhibiting green fluorescence.
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c3 during normalization. A split Cas9 gate with one half under p hCEA and the other activated by TWIST1 detected cells undergoing epithelial to mesenchymal transition by reporter activation. Derived from claim c3. Quoted text: we designed a logic gate where one half of Cas9 is expressed under the p hCEA, while the other is activated by TWIST1. The results showed that cells undergoing EMT effectively activated the reporter.
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c4 during normalization. Combining epithelial origin and EMT inputs produced a split Cas9 logic gate that activated the reporter only in cancer epithelial cells undergoing EMT. Derived from claim c4. Quoted text: we combined the two inputs (epithelial origin and EMT) to create a new logic gate, where only cancer epithelial cells undergoing EMT activated the reporter
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c5 during normalization. The split Cas9 logic gate functioned as a sensor of cell-cell fusion in induced and naturally occurring scenarios, with fusion producing multinucleated syncytia and reporter fluorescence. Derived from claim c5. Quoted text: we applied the split-Cas9 logic gate as a sensor of cell-cell fusion, both in induced and naturally occurring scenarios. Each cell type expressed one half of split Cas9, and the induction of fusion resulted in the appearance of multinucleated syncytia and the fluorescent reporter.
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c2 during normalization. A split Cas9 gate with one half under an epithelial cancer-specific promoter and the other under a universal promoter activated the reporter only in cancer cells with epithelial origin. Derived from claim c2. Quoted text: First, we positioned the complementing halves of split Cas9 under different promoters-one specific to cancer cells of epithelial origin (p hCEA) and the other a universal promoter... Consequently, only cancer cells with an epithelial origin activated the reporter, exhibiting green fluorescence.
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c3 during normalization. A split Cas9 gate with one half under p hCEA and the other activated by TWIST1 detected cells undergoing epithelial to mesenchymal transition by reporter activation. Derived from claim c3. Quoted text: we designed a logic gate where one half of Cas9 is expressed under the p hCEA, while the other is activated by TWIST1. The results showed that cells undergoing EMT effectively activated the reporter.
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c4 during normalization. Combining epithelial origin and EMT inputs produced a split Cas9 logic gate that activated the reporter only in cancer epithelial cells undergoing EMT. Derived from claim c4. Quoted text: we combined the two inputs (epithelial origin and EMT) to create a new logic gate, where only cancer epithelial cells undergoing EMT activated the reporter
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c5 during normalization. The split Cas9 logic gate functioned as a sensor of cell-cell fusion in induced and naturally occurring scenarios, with fusion producing multinucleated syncytia and reporter fluorescence. Derived from claim c5. Quoted text: we applied the split-Cas9 logic gate as a sensor of cell-cell fusion, both in induced and naturally occurring scenarios. Each cell type expressed one half of split Cas9, and the induction of fusion resulted in the appearance of multinucleated syncytia and the fluorescent reporter.
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c2 during normalization. A split Cas9 gate with one half under an epithelial cancer-specific promoter and the other under a universal promoter activated the reporter only in cancer cells with epithelial origin. Derived from claim c2. Quoted text: First, we positioned the complementing halves of split Cas9 under different promoters-one specific to cancer cells of epithelial origin (p hCEA) and the other a universal promoter... Consequently, only cancer cells with an epithelial origin activated the reporter, exhibiting green fluorescence.
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c3 during normalization. A split Cas9 gate with one half under p hCEA and the other activated by TWIST1 detected cells undergoing epithelial to mesenchymal transition by reporter activation. Derived from claim c3. Quoted text: we designed a logic gate where one half of Cas9 is expressed under the p hCEA, while the other is activated by TWIST1. The results showed that cells undergoing EMT effectively activated the reporter.
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c4 during normalization. Combining epithelial origin and EMT inputs produced a split Cas9 logic gate that activated the reporter only in cancer epithelial cells undergoing EMT. Derived from claim c4. Quoted text: we combined the two inputs (epithelial origin and EMT) to create a new logic gate, where only cancer epithelial cells undergoing EMT activated the reporter
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c5 during normalization. The split Cas9 logic gate functioned as a sensor of cell-cell fusion in induced and naturally occurring scenarios, with fusion producing multinucleated syncytia and reporter fluorescence. Derived from claim c5. Quoted text: we applied the split-Cas9 logic gate as a sensor of cell-cell fusion, both in induced and naturally occurring scenarios. Each cell type expressed one half of split Cas9, and the induction of fusion resulted in the appearance of multinucleated syncytia and the fluorescent reporter.
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c2 during normalization. A split Cas9 gate with one half under an epithelial cancer-specific promoter and the other under a universal promoter activated the reporter only in cancer cells with epithelial origin. Derived from claim c2. Quoted text: First, we positioned the complementing halves of split Cas9 under different promoters-one specific to cancer cells of epithelial origin (p hCEA) and the other a universal promoter... Consequently, only cancer cells with an epithelial origin activated the reporter, exhibiting green fluorescence.
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c3 during normalization. A split Cas9 gate with one half under p hCEA and the other activated by TWIST1 detected cells undergoing epithelial to mesenchymal transition by reporter activation. Derived from claim c3. Quoted text: we designed a logic gate where one half of Cas9 is expressed under the p hCEA, while the other is activated by TWIST1. The results showed that cells undergoing EMT effectively activated the reporter.
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c4 during normalization. Combining epithelial origin and EMT inputs produced a split Cas9 logic gate that activated the reporter only in cancer epithelial cells undergoing EMT. Derived from claim c4. Quoted text: we combined the two inputs (epithelial origin and EMT) to create a new logic gate, where only cancer epithelial cells undergoing EMT activated the reporter
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c5 during normalization. The split Cas9 logic gate functioned as a sensor of cell-cell fusion in induced and naturally occurring scenarios, with fusion producing multinucleated syncytia and reporter fluorescence. Derived from claim c5. Quoted text: we applied the split-Cas9 logic gate as a sensor of cell-cell fusion, both in induced and naturally occurring scenarios. Each cell type expressed one half of split Cas9, and the induction of fusion resulted in the appearance of multinucleated syncytia and the fluorescent reporter.
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c2 during normalization. A split Cas9 gate with one half under an epithelial cancer-specific promoter and the other under a universal promoter activated the reporter only in cancer cells with epithelial origin. Derived from claim c2. Quoted text: First, we positioned the complementing halves of split Cas9 under different promoters-one specific to cancer cells of epithelial origin (p hCEA) and the other a universal promoter... Consequently, only cancer cells with an epithelial origin activated the reporter, exhibiting green fluorescence.
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c3 during normalization. A split Cas9 gate with one half under p hCEA and the other activated by TWIST1 detected cells undergoing epithelial to mesenchymal transition by reporter activation. Derived from claim c3. Quoted text: we designed a logic gate where one half of Cas9 is expressed under the p hCEA, while the other is activated by TWIST1. The results showed that cells undergoing EMT effectively activated the reporter.
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c4 during normalization. Combining epithelial origin and EMT inputs produced a split Cas9 logic gate that activated the reporter only in cancer epithelial cells undergoing EMT. Derived from claim c4. Quoted text: we combined the two inputs (epithelial origin and EMT) to create a new logic gate, where only cancer epithelial cells undergoing EMT activated the reporter
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c5 during normalization. The split Cas9 logic gate functioned as a sensor of cell-cell fusion in induced and naturally occurring scenarios, with fusion producing multinucleated syncytia and reporter fluorescence. Derived from claim c5. Quoted text: we applied the split-Cas9 logic gate as a sensor of cell-cell fusion, both in induced and naturally occurring scenarios. Each cell type expressed one half of split Cas9, and the induction of fusion resulted in the appearance of multinucleated syncytia and the fluorescent reporter.
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c2 during normalization. A split Cas9 gate with one half under an epithelial cancer-specific promoter and the other under a universal promoter activated the reporter only in cancer cells with epithelial origin. Derived from claim c2. Quoted text: First, we positioned the complementing halves of split Cas9 under different promoters-one specific to cancer cells of epithelial origin (p hCEA) and the other a universal promoter... Consequently, only cancer cells with an epithelial origin activated the reporter, exhibiting green fluorescence.
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c3 during normalization. A split Cas9 gate with one half under p hCEA and the other activated by TWIST1 detected cells undergoing epithelial to mesenchymal transition by reporter activation. Derived from claim c3. Quoted text: we designed a logic gate where one half of Cas9 is expressed under the p hCEA, while the other is activated by TWIST1. The results showed that cells undergoing EMT effectively activated the reporter.
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c4 during normalization. Combining epithelial origin and EMT inputs produced a split Cas9 logic gate that activated the reporter only in cancer epithelial cells undergoing EMT. Derived from claim c4. Quoted text: we combined the two inputs (epithelial origin and EMT) to create a new logic gate, where only cancer epithelial cells undergoing EMT activated the reporter
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c5 during normalization. The split Cas9 logic gate functioned as a sensor of cell-cell fusion in induced and naturally occurring scenarios, with fusion producing multinucleated syncytia and reporter fluorescence. Derived from claim c5. Quoted text: we applied the split-Cas9 logic gate as a sensor of cell-cell fusion, both in induced and naturally occurring scenarios. Each cell type expressed one half of split Cas9, and the induction of fusion resulted in the appearance of multinucleated syncytia and the fluorescent reporter.
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c2 during normalization. A split Cas9 gate with one half under an epithelial cancer-specific promoter and the other under a universal promoter activated the reporter only in cancer cells with epithelial origin. Derived from claim c2. Quoted text: First, we positioned the complementing halves of split Cas9 under different promoters-one specific to cancer cells of epithelial origin (p hCEA) and the other a universal promoter... Consequently, only cancer cells with an epithelial origin activated the reporter, exhibiting green fluorescence.
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c3 during normalization. A split Cas9 gate with one half under p hCEA and the other activated by TWIST1 detected cells undergoing epithelial to mesenchymal transition by reporter activation. Derived from claim c3. Quoted text: we designed a logic gate where one half of Cas9 is expressed under the p hCEA, while the other is activated by TWIST1. The results showed that cells undergoing EMT effectively activated the reporter.
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c4 during normalization. Combining epithelial origin and EMT inputs produced a split Cas9 logic gate that activated the reporter only in cancer epithelial cells undergoing EMT. Derived from claim c4. Quoted text: we combined the two inputs (epithelial origin and EMT) to create a new logic gate, where only cancer epithelial cells undergoing EMT activated the reporter
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c5 during normalization. The split Cas9 logic gate functioned as a sensor of cell-cell fusion in induced and naturally occurring scenarios, with fusion producing multinucleated syncytia and reporter fluorescence. Derived from claim c5. Quoted text: we applied the split-Cas9 logic gate as a sensor of cell-cell fusion, both in induced and naturally occurring scenarios. Each cell type expressed one half of split Cas9, and the induction of fusion resulted in the appearance of multinucleated syncytia and the fluorescent reporter.
Source:
Supporting Sources
Source 2primary paper2023Scientific Reports
Ranked Claims
Self-assembling inteins facilitated reconstitution of the split Cas9 halves.
The use of self-assembling inteins facilitated the reconstitution of the Cas9 halves.
Self-assembling inteins facilitated reconstitution of the split Cas9 halves.
The use of self-assembling inteins facilitated the reconstitution of the Cas9 halves.
Self-assembling inteins facilitated reconstitution of the split Cas9 halves.
The use of self-assembling inteins facilitated the reconstitution of the Cas9 halves.
Self-assembling inteins facilitated reconstitution of the split Cas9 halves.
The use of self-assembling inteins facilitated the reconstitution of the Cas9 halves.
Self-assembling inteins facilitated reconstitution of the split Cas9 halves.
The use of self-assembling inteins facilitated the reconstitution of the Cas9 halves.
Self-assembling inteins facilitated reconstitution of the split Cas9 halves.
The use of self-assembling inteins facilitated the reconstitution of the Cas9 halves.
Self-assembling inteins facilitated reconstitution of the split Cas9 halves.
The use of self-assembling inteins facilitated the reconstitution of the Cas9 halves.
A split Cas9 gate with one half under an epithelial cancer-specific promoter and the other under a universal promoter activated the reporter only in cancer cells with epithelial origin.
First, we positioned the complementing halves of split Cas9 under different promoters-one specific to cancer cells of epithelial origin (p hCEA) and the other a universal promoter... Consequently, only cancer cells with an epithelial origin activated the reporter, exhibiting green fluorescence.
A split Cas9 gate with one half under an epithelial cancer-specific promoter and the other under a universal promoter activated the reporter only in cancer cells with epithelial origin.
First, we positioned the complementing halves of split Cas9 under different promoters-one specific to cancer cells of epithelial origin (p hCEA) and the other a universal promoter... Consequently, only cancer cells with an epithelial origin activated the reporter, exhibiting green fluorescence.
A split Cas9 gate with one half under an epithelial cancer-specific promoter and the other under a universal promoter activated the reporter only in cancer cells with epithelial origin.
First, we positioned the complementing halves of split Cas9 under different promoters-one specific to cancer cells of epithelial origin (p hCEA) and the other a universal promoter... Consequently, only cancer cells with an epithelial origin activated the reporter, exhibiting green fluorescence.
A split Cas9 gate with one half under an epithelial cancer-specific promoter and the other under a universal promoter activated the reporter only in cancer cells with epithelial origin.
First, we positioned the complementing halves of split Cas9 under different promoters-one specific to cancer cells of epithelial origin (p hCEA) and the other a universal promoter... Consequently, only cancer cells with an epithelial origin activated the reporter, exhibiting green fluorescence.
A split Cas9 gate with one half under an epithelial cancer-specific promoter and the other under a universal promoter activated the reporter only in cancer cells with epithelial origin.
First, we positioned the complementing halves of split Cas9 under different promoters-one specific to cancer cells of epithelial origin (p hCEA) and the other a universal promoter... Consequently, only cancer cells with an epithelial origin activated the reporter, exhibiting green fluorescence.
A split Cas9 gate with one half under an epithelial cancer-specific promoter and the other under a universal promoter activated the reporter only in cancer cells with epithelial origin.
First, we positioned the complementing halves of split Cas9 under different promoters-one specific to cancer cells of epithelial origin (p hCEA) and the other a universal promoter... Consequently, only cancer cells with an epithelial origin activated the reporter, exhibiting green fluorescence.
A split Cas9 gate with one half under an epithelial cancer-specific promoter and the other under a universal promoter activated the reporter only in cancer cells with epithelial origin.
First, we positioned the complementing halves of split Cas9 under different promoters-one specific to cancer cells of epithelial origin (p hCEA) and the other a universal promoter... Consequently, only cancer cells with an epithelial origin activated the reporter, exhibiting green fluorescence.
A split Cas9 gate with one half under p hCEA and the other activated by TWIST1 detected cells undergoing epithelial to mesenchymal transition by reporter activation.
we designed a logic gate where one half of Cas9 is expressed under the p hCEA, while the other is activated by TWIST1. The results showed that cells undergoing EMT effectively activated the reporter.
A split Cas9 gate with one half under p hCEA and the other activated by TWIST1 detected cells undergoing epithelial to mesenchymal transition by reporter activation.
we designed a logic gate where one half of Cas9 is expressed under the p hCEA, while the other is activated by TWIST1. The results showed that cells undergoing EMT effectively activated the reporter.
A split Cas9 gate with one half under p hCEA and the other activated by TWIST1 detected cells undergoing epithelial to mesenchymal transition by reporter activation.
we designed a logic gate where one half of Cas9 is expressed under the p hCEA, while the other is activated by TWIST1. The results showed that cells undergoing EMT effectively activated the reporter.
A split Cas9 gate with one half under p hCEA and the other activated by TWIST1 detected cells undergoing epithelial to mesenchymal transition by reporter activation.
we designed a logic gate where one half of Cas9 is expressed under the p hCEA, while the other is activated by TWIST1. The results showed that cells undergoing EMT effectively activated the reporter.
A split Cas9 gate with one half under p hCEA and the other activated by TWIST1 detected cells undergoing epithelial to mesenchymal transition by reporter activation.
we designed a logic gate where one half of Cas9 is expressed under the p hCEA, while the other is activated by TWIST1. The results showed that cells undergoing EMT effectively activated the reporter.
A split Cas9 gate with one half under p hCEA and the other activated by TWIST1 detected cells undergoing epithelial to mesenchymal transition by reporter activation.
we designed a logic gate where one half of Cas9 is expressed under the p hCEA, while the other is activated by TWIST1. The results showed that cells undergoing EMT effectively activated the reporter.
A split Cas9 gate with one half under p hCEA and the other activated by TWIST1 detected cells undergoing epithelial to mesenchymal transition by reporter activation.
we designed a logic gate where one half of Cas9 is expressed under the p hCEA, while the other is activated by TWIST1. The results showed that cells undergoing EMT effectively activated the reporter.
Combining epithelial origin and EMT inputs produced a split Cas9 logic gate that activated the reporter only in cancer epithelial cells undergoing EMT.
we combined the two inputs (epithelial origin and EMT) to create a new logic gate, where only cancer epithelial cells undergoing EMT activated the reporter
Combining epithelial origin and EMT inputs produced a split Cas9 logic gate that activated the reporter only in cancer epithelial cells undergoing EMT.
we combined the two inputs (epithelial origin and EMT) to create a new logic gate, where only cancer epithelial cells undergoing EMT activated the reporter
Combining epithelial origin and EMT inputs produced a split Cas9 logic gate that activated the reporter only in cancer epithelial cells undergoing EMT.
we combined the two inputs (epithelial origin and EMT) to create a new logic gate, where only cancer epithelial cells undergoing EMT activated the reporter
Combining epithelial origin and EMT inputs produced a split Cas9 logic gate that activated the reporter only in cancer epithelial cells undergoing EMT.
we combined the two inputs (epithelial origin and EMT) to create a new logic gate, where only cancer epithelial cells undergoing EMT activated the reporter
Combining epithelial origin and EMT inputs produced a split Cas9 logic gate that activated the reporter only in cancer epithelial cells undergoing EMT.
we combined the two inputs (epithelial origin and EMT) to create a new logic gate, where only cancer epithelial cells undergoing EMT activated the reporter
Combining epithelial origin and EMT inputs produced a split Cas9 logic gate that activated the reporter only in cancer epithelial cells undergoing EMT.
we combined the two inputs (epithelial origin and EMT) to create a new logic gate, where only cancer epithelial cells undergoing EMT activated the reporter
Combining epithelial origin and EMT inputs produced a split Cas9 logic gate that activated the reporter only in cancer epithelial cells undergoing EMT.
we combined the two inputs (epithelial origin and EMT) to create a new logic gate, where only cancer epithelial cells undergoing EMT activated the reporter
The split Cas9 logic gate functioned as a sensor of cell-cell fusion in induced and naturally occurring scenarios, with fusion producing multinucleated syncytia and reporter fluorescence.
we applied the split-Cas9 logic gate as a sensor of cell-cell fusion, both in induced and naturally occurring scenarios. Each cell type expressed one half of split Cas9, and the induction of fusion resulted in the appearance of multinucleated syncytia and the fluorescent reporter.
The split Cas9 logic gate functioned as a sensor of cell-cell fusion in induced and naturally occurring scenarios, with fusion producing multinucleated syncytia and reporter fluorescence.
we applied the split-Cas9 logic gate as a sensor of cell-cell fusion, both in induced and naturally occurring scenarios. Each cell type expressed one half of split Cas9, and the induction of fusion resulted in the appearance of multinucleated syncytia and the fluorescent reporter.
The split Cas9 logic gate functioned as a sensor of cell-cell fusion in induced and naturally occurring scenarios, with fusion producing multinucleated syncytia and reporter fluorescence.
we applied the split-Cas9 logic gate as a sensor of cell-cell fusion, both in induced and naturally occurring scenarios. Each cell type expressed one half of split Cas9, and the induction of fusion resulted in the appearance of multinucleated syncytia and the fluorescent reporter.
The split Cas9 logic gate functioned as a sensor of cell-cell fusion in induced and naturally occurring scenarios, with fusion producing multinucleated syncytia and reporter fluorescence.
we applied the split-Cas9 logic gate as a sensor of cell-cell fusion, both in induced and naturally occurring scenarios. Each cell type expressed one half of split Cas9, and the induction of fusion resulted in the appearance of multinucleated syncytia and the fluorescent reporter.
The split Cas9 logic gate functioned as a sensor of cell-cell fusion in induced and naturally occurring scenarios, with fusion producing multinucleated syncytia and reporter fluorescence.
we applied the split-Cas9 logic gate as a sensor of cell-cell fusion, both in induced and naturally occurring scenarios. Each cell type expressed one half of split Cas9, and the induction of fusion resulted in the appearance of multinucleated syncytia and the fluorescent reporter.
The split Cas9 logic gate functioned as a sensor of cell-cell fusion in induced and naturally occurring scenarios, with fusion producing multinucleated syncytia and reporter fluorescence.
we applied the split-Cas9 logic gate as a sensor of cell-cell fusion, both in induced and naturally occurring scenarios. Each cell type expressed one half of split Cas9, and the induction of fusion resulted in the appearance of multinucleated syncytia and the fluorescent reporter.
The split Cas9 logic gate functioned as a sensor of cell-cell fusion in induced and naturally occurring scenarios, with fusion producing multinucleated syncytia and reporter fluorescence.
we applied the split-Cas9 logic gate as a sensor of cell-cell fusion, both in induced and naturally occurring scenarios. Each cell type expressed one half of split Cas9, and the induction of fusion resulted in the appearance of multinucleated syncytia and the fluorescent reporter.
An adaptation of split Cas9 can generate logic gates that sense biological events.
we present an adaptation of split Cas9 to generate logic gates capable of sensing biological events
An adaptation of split Cas9 can generate logic gates that sense biological events.
we present an adaptation of split Cas9 to generate logic gates capable of sensing biological events
An adaptation of split Cas9 can generate logic gates that sense biological events.
we present an adaptation of split Cas9 to generate logic gates capable of sensing biological events
An adaptation of split Cas9 can generate logic gates that sense biological events.
we present an adaptation of split Cas9 to generate logic gates capable of sensing biological events
An adaptation of split Cas9 can generate logic gates that sense biological events.
we present an adaptation of split Cas9 to generate logic gates capable of sensing biological events
An adaptation of split Cas9 can generate logic gates that sense biological events.
we present an adaptation of split Cas9 to generate logic gates capable of sensing biological events
An adaptation of split Cas9 can generate logic gates that sense biological events.
we present an adaptation of split Cas9 to generate logic gates capable of sensing biological events
The split Cas9 system is presented as simple and potentially integrable into various cellular processes as both a sensor and an actuator.
The simplicity of the split Cas9 system presented here allows for its integration into various cellular processes, not only as a sensor but also as an actuator.
The split Cas9 system is presented as simple and potentially integrable into various cellular processes as both a sensor and an actuator.
The simplicity of the split Cas9 system presented here allows for its integration into various cellular processes, not only as a sensor but also as an actuator.
The split Cas9 system is presented as simple and potentially integrable into various cellular processes as both a sensor and an actuator.
The simplicity of the split Cas9 system presented here allows for its integration into various cellular processes, not only as a sensor but also as an actuator.
The split Cas9 system is presented as simple and potentially integrable into various cellular processes as both a sensor and an actuator.
The simplicity of the split Cas9 system presented here allows for its integration into various cellular processes, not only as a sensor but also as an actuator.
The split Cas9 system is presented as simple and potentially integrable into various cellular processes as both a sensor and an actuator.
The simplicity of the split Cas9 system presented here allows for its integration into various cellular processes, not only as a sensor but also as an actuator.
The split Cas9 system is presented as simple and potentially integrable into various cellular processes as both a sensor and an actuator.
The simplicity of the split Cas9 system presented here allows for its integration into various cellular processes, not only as a sensor but also as an actuator.
The split Cas9 system is presented as simple and potentially integrable into various cellular processes as both a sensor and an actuator.
The simplicity of the split Cas9 system presented here allows for its integration into various cellular processes, not only as a sensor but also as an actuator.
The review covers split-Cas9 constructs, inteins, small molecules, protein-based dimerizing domains, and light-inducible systems as strategies to modulate Cas9-mediated genome editing.
Here, we review recent advancements to modulate Cas9-mediated genome editing by engineering split-Cas9 constructs, inteins, small molecules, protein-based dimerizing domains, and light-inducible systems.
Approval Evidence
2 sources8 linked approval claimsfirst-pass slugs split-cas9, split-cas9-logic-gate
we present an adaptation of split Cas9 to generate logic gates capable of sensing biological events
Source:
Here, we review recent advancements to modulate Cas9-mediated genome editing by engineering split-Cas9 constructs...
Source:
design mechanismsupports
Self-assembling inteins facilitated reconstitution of the split Cas9 halves.
The use of self-assembling inteins facilitated the reconstitution of the Cas9 halves.
Source:
functional resultsupports
A split Cas9 gate with one half under an epithelial cancer-specific promoter and the other under a universal promoter activated the reporter only in cancer cells with epithelial origin.
First, we positioned the complementing halves of split Cas9 under different promoters-one specific to cancer cells of epithelial origin (p hCEA) and the other a universal promoter... Consequently, only cancer cells with an epithelial origin activated the reporter, exhibiting green fluorescence.
Source:
functional resultsupports
A split Cas9 gate with one half under p hCEA and the other activated by TWIST1 detected cells undergoing epithelial to mesenchymal transition by reporter activation.
we designed a logic gate where one half of Cas9 is expressed under the p hCEA, while the other is activated by TWIST1. The results showed that cells undergoing EMT effectively activated the reporter.
Source:
functional resultsupports
Combining epithelial origin and EMT inputs produced a split Cas9 logic gate that activated the reporter only in cancer epithelial cells undergoing EMT.
we combined the two inputs (epithelial origin and EMT) to create a new logic gate, where only cancer epithelial cells undergoing EMT activated the reporter
Source:
functional resultsupports
The split Cas9 logic gate functioned as a sensor of cell-cell fusion in induced and naturally occurring scenarios, with fusion producing multinucleated syncytia and reporter fluorescence.
we applied the split-Cas9 logic gate as a sensor of cell-cell fusion, both in induced and naturally occurring scenarios. Each cell type expressed one half of split Cas9, and the induction of fusion resulted in the appearance of multinucleated syncytia and the fluorescent reporter.
Source:
tool capabilitysupports
An adaptation of split Cas9 can generate logic gates that sense biological events.
we present an adaptation of split Cas9 to generate logic gates capable of sensing biological events
Source:
tool capabilitysupports
The split Cas9 system is presented as simple and potentially integrable into various cellular processes as both a sensor and an actuator.
The simplicity of the split Cas9 system presented here allows for its integration into various cellular processes, not only as a sensor but also as an actuator.
Source:
review scope statementsupports
The review covers split-Cas9 constructs, inteins, small molecules, protein-based dimerizing domains, and light-inducible systems as strategies to modulate Cas9-mediated genome editing.
Here, we review recent advancements to modulate Cas9-mediated genome editing by engineering split-Cas9 constructs, inteins, small molecules, protein-based dimerizing domains, and light-inducible systems.
Source:
Comparisons
Source-backed strengths
The reported system detected both induced and naturally occurring cell-cell fusion, producing reporter fluorescence in multinucleated syncytia. It also supported promoter-defined logic configurations, including gates for epithelial cancer cells, EMT-associated states via TWIST1-linked activation, and a combined gate that responded only in cancer epithelial cells undergoing EMT.
Ranked Citations
- 1.
- 2.