Source 1primary paper2019Communications Biology
Toolkit/split sfCherry3 variants
split sfCherry3 variants
Also known as: sfCherry3 variants, split sfCherry3
Taxonomy: Mechanism Branch / Component. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Split sfCherry3 variants are engineered split red fluorescent proteins derived from sfCherry through directed evolution. They were reported to show substantially enhanced overall brightness and to support visualization of endogenous proteins and neuronal synapses.
Usefulness & Problems
Why this is useful
These variants are useful as brighter split fluorescent protein tools for protein tagging and imaging applications where red fluorescence is advantageous. The reported work indicates utility for tagging endogenous proteins by gene editing and for multiplexed visualization of neuronal synapses in living Caenorhabditis elegans.
Source:
for multiplexed visualization of neuronal synapses in living C. elegans
Source:
facilitating the tagging of endogenous proteins by gene editing
Source:
Based on sfCherry3, we have further developed a new red-colored trans-synaptic marker called Neuroligin-1 sfCherry3 Linker Across Synaptic Partners (NLG-1 CLASP)
Problem solved
They address the limited brightness of earlier split red fluorescent proteins, which can constrain detection of tagged endogenous proteins and other low-abundance targets. The source specifically states that two split sfCherry3 variants were obtained with substantially enhanced overall brightness.
Source:
for multiplexed visualization of neuronal synapses in living C. elegans
Source:
facilitating the tagging of endogenous proteins by gene editing
Source:
Based on sfCherry3, we have further developed a new red-colored trans-synaptic marker called Neuroligin-1 sfCherry3 Linker Across Synaptic Partners (NLG-1 CLASP)
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Component: A low-level protein part used inside a larger architecture that realizes a mechanism.
Mechanisms
protein-protein interaction-assisted complementationprotein-protein interaction-assisted complementation via spytag/spycatchersplit fluorescent protein complementationTechniques
Directed EvolutionTarget processes
editingImplementation Constraints
The tool is a split fluorescent protein system based on sfCherry3 variants, implying use as separable fragments that reconstitute fluorescence upon complementation. The source also notes SpyTag/SpyCatcher interaction as an approach used to improve split fluorescent proteins, but the provided evidence does not specify exact construct architectures, fragment boundaries, or expression and delivery conditions for these variants.
The supplied evidence does not provide quantitative brightness values, complementation efficiency, maturation kinetics, or photostability measurements. Independent replication is not documented in the provided material, and validation evidence is limited to the cited 2019 study and its described applications.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
NLG-1 CLASP enables multiplexed visualization of neuronal synapses in living C. elegans.
for multiplexed visualization of neuronal synapses in living C. elegans
NLG-1 CLASP enables multiplexed visualization of neuronal synapses in living C. elegans.
for multiplexed visualization of neuronal synapses in living C. elegans
NLG-1 CLASP enables multiplexed visualization of neuronal synapses in living C. elegans.
for multiplexed visualization of neuronal synapses in living C. elegans
NLG-1 CLASP enables multiplexed visualization of neuronal synapses in living C. elegans.
for multiplexed visualization of neuronal synapses in living C. elegans
NLG-1 CLASP enables multiplexed visualization of neuronal synapses in living C. elegans.
for multiplexed visualization of neuronal synapses in living C. elegans
NLG-1 CLASP enables multiplexed visualization of neuronal synapses in living C. elegans.
for multiplexed visualization of neuronal synapses in living C. elegans
NLG-1 CLASP enables multiplexed visualization of neuronal synapses in living C. elegans.
for multiplexed visualization of neuronal synapses in living C. elegans
The split sfCherry3 variants facilitate tagging of endogenous proteins by gene editing.
facilitating the tagging of endogenous proteins by gene editing
The split sfCherry3 variants facilitate tagging of endogenous proteins by gene editing.
facilitating the tagging of endogenous proteins by gene editing
The split sfCherry3 variants facilitate tagging of endogenous proteins by gene editing.
facilitating the tagging of endogenous proteins by gene editing
The split sfCherry3 variants facilitate tagging of endogenous proteins by gene editing.
facilitating the tagging of endogenous proteins by gene editing
The split sfCherry3 variants facilitate tagging of endogenous proteins by gene editing.
facilitating the tagging of endogenous proteins by gene editing
The split sfCherry3 variants facilitate tagging of endogenous proteins by gene editing.
facilitating the tagging of endogenous proteins by gene editing
The split sfCherry3 variants facilitate tagging of endogenous proteins by gene editing.
facilitating the tagging of endogenous proteins by gene editing
SpyTag/SpyCatcher interaction and directed evolution were demonstrated as two approaches to improve split fluorescent proteins.
we have demonstrated two approaches to improve split FPs: assistance through SpyTag/SpyCatcher interaction and directed evolution
SpyTag/SpyCatcher interaction and directed evolution were demonstrated as two approaches to improve split fluorescent proteins.
we have demonstrated two approaches to improve split FPs: assistance through SpyTag/SpyCatcher interaction and directed evolution
SpyTag/SpyCatcher interaction and directed evolution were demonstrated as two approaches to improve split fluorescent proteins.
we have demonstrated two approaches to improve split FPs: assistance through SpyTag/SpyCatcher interaction and directed evolution
SpyTag/SpyCatcher interaction and directed evolution were demonstrated as two approaches to improve split fluorescent proteins.
we have demonstrated two approaches to improve split FPs: assistance through SpyTag/SpyCatcher interaction and directed evolution
SpyTag/SpyCatcher interaction and directed evolution were demonstrated as two approaches to improve split fluorescent proteins.
we have demonstrated two approaches to improve split FPs: assistance through SpyTag/SpyCatcher interaction and directed evolution
SpyTag/SpyCatcher interaction and directed evolution were demonstrated as two approaches to improve split fluorescent proteins.
we have demonstrated two approaches to improve split FPs: assistance through SpyTag/SpyCatcher interaction and directed evolution
SpyTag/SpyCatcher interaction and directed evolution were demonstrated as two approaches to improve split fluorescent proteins.
we have demonstrated two approaches to improve split FPs: assistance through SpyTag/SpyCatcher interaction and directed evolution
Directed evolution yielded two split sfCherry3 variants with substantially enhanced overall brightness.
The latter has yielded two split sfCherry3 variants with substantially enhanced overall brightness
Directed evolution yielded two split sfCherry3 variants with substantially enhanced overall brightness.
The latter has yielded two split sfCherry3 variants with substantially enhanced overall brightness
Directed evolution yielded two split sfCherry3 variants with substantially enhanced overall brightness.
The latter has yielded two split sfCherry3 variants with substantially enhanced overall brightness
Directed evolution yielded two split sfCherry3 variants with substantially enhanced overall brightness.
The latter has yielded two split sfCherry3 variants with substantially enhanced overall brightness
Directed evolution yielded two split sfCherry3 variants with substantially enhanced overall brightness.
The latter has yielded two split sfCherry3 variants with substantially enhanced overall brightness
Directed evolution yielded two split sfCherry3 variants with substantially enhanced overall brightness.
The latter has yielded two split sfCherry3 variants with substantially enhanced overall brightness
Directed evolution yielded two split sfCherry3 variants with substantially enhanced overall brightness.
The latter has yielded two split sfCherry3 variants with substantially enhanced overall brightness
Based on sfCherry3, the authors developed a new red-colored trans-synaptic marker called NLG-1 CLASP.
Based on sfCherry3, we have further developed a new red-colored trans-synaptic marker called Neuroligin-1 sfCherry3 Linker Across Synaptic Partners (NLG-1 CLASP)
Based on sfCherry3, the authors developed a new red-colored trans-synaptic marker called NLG-1 CLASP.
Based on sfCherry3, we have further developed a new red-colored trans-synaptic marker called Neuroligin-1 sfCherry3 Linker Across Synaptic Partners (NLG-1 CLASP)
Based on sfCherry3, the authors developed a new red-colored trans-synaptic marker called NLG-1 CLASP.
Based on sfCherry3, we have further developed a new red-colored trans-synaptic marker called Neuroligin-1 sfCherry3 Linker Across Synaptic Partners (NLG-1 CLASP)
Based on sfCherry3, the authors developed a new red-colored trans-synaptic marker called NLG-1 CLASP.
Based on sfCherry3, we have further developed a new red-colored trans-synaptic marker called Neuroligin-1 sfCherry3 Linker Across Synaptic Partners (NLG-1 CLASP)
Based on sfCherry3, the authors developed a new red-colored trans-synaptic marker called NLG-1 CLASP.
Based on sfCherry3, we have further developed a new red-colored trans-synaptic marker called Neuroligin-1 sfCherry3 Linker Across Synaptic Partners (NLG-1 CLASP)
Based on sfCherry3, the authors developed a new red-colored trans-synaptic marker called NLG-1 CLASP.
Based on sfCherry3, we have further developed a new red-colored trans-synaptic marker called Neuroligin-1 sfCherry3 Linker Across Synaptic Partners (NLG-1 CLASP)
Based on sfCherry3, the authors developed a new red-colored trans-synaptic marker called NLG-1 CLASP.
Based on sfCherry3, we have further developed a new red-colored trans-synaptic marker called Neuroligin-1 sfCherry3 Linker Across Synaptic Partners (NLG-1 CLASP)
Approval Evidence
1 source3 linked approval claimsfirst-pass slug split-sfcherry3-variants
The latter has yielded two split sfCherry3 variants with substantially enhanced overall brightness
Source:
application enablementsupports
The split sfCherry3 variants facilitate tagging of endogenous proteins by gene editing.
facilitating the tagging of endogenous proteins by gene editing
Source:
performance improvementsupports
Directed evolution yielded two split sfCherry3 variants with substantially enhanced overall brightness.
The latter has yielded two split sfCherry3 variants with substantially enhanced overall brightness
Source:
tool developmentsupports
Based on sfCherry3, the authors developed a new red-colored trans-synaptic marker called NLG-1 CLASP.
Based on sfCherry3, we have further developed a new red-colored trans-synaptic marker called Neuroligin-1 sfCherry3 Linker Across Synaptic Partners (NLG-1 CLASP)
Source:
Comparisons
Source-backed strengths
The main reported strength is substantially enhanced overall brightness relative to prior split sfCherry designs. The variants were also demonstrated in application contexts including endogenous protein tagging by gene editing and NLG-1 CLASP-based multiplexed synapse visualization in living C. elegans.
Source:
The latter has yielded two split sfCherry3 variants with substantially enhanced overall brightness
Ranked Citations
- 1.