Source 1primary paper2019Biotechnology and Bioengineering
Toolkit/split synthetic zinc-finger transcription factor
split synthetic zinc-finger transcription factor
Also known as: split synthetic zinc-finger TF
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The split synthetic zinc-finger transcription factor is a light-controlled transcriptional switch developed for Saccharomyces cerevisiae. Its activity is reconstituted from split components through CRY2- and CIB1-mediated light-induced dimerization, enabling optical control of gene expression.
Usefulness & Problems
Why this is useful
This tool supports optogenetic control of transcription in S. cerevisiae and was developed in the context of a yeast optogenetic toolkit for rapid circuit assembly. The cited study states that it enables rapid generation and prototyping of light-controlled gene expression circuits in yeast.
Source:
This study allows for rapid generation and prototyping of optogenetic circuits to control gene expression in S. cerevisiae.
Problem solved
It addresses the need for a modular method to control gene expression with light in Saccharomyces cerevisiae. Specifically, it provides a way to reconstitute transcription factor activity conditionally through illumination rather than constitutive assembly.
Source:
This study allows for rapid generation and prototyping of optogenetic circuits to control gene expression in S. cerevisiae.
Problem links
Need precise spatiotemporal control with light input
DerivedThe split synthetic zinc-finger transcription factor is a light-controlled, multi-component transcriptional switch for Saccharomyces cerevisiae. Its activity is reconstituted by CRY2- and CIB1-mediated light-induced dimerization, enabling optical control of gene expression from a synthetic promoter.
Need tighter control over gene expression timing or amplitude
DerivedThe split synthetic zinc-finger transcription factor is a light-controlled, multi-component transcriptional switch for Saccharomyces cerevisiae. Its activity is reconstituted by CRY2- and CIB1-mediated light-induced dimerization, enabling optical control of gene expression from a synthetic promoter.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
HeterodimerizationHeterodimerizationlight-induced heterodimerizationlight-induced heterodimerizationtranscription factor reconstitutiontranscription factor reconstitutionTechniques
No technique tags yet.
Target processes
transcriptionInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: multi component delivery burdenimplementation constraint: spectral hardware requirementoperating role: regulatorswitch architecture: multi componentswitch architecture: recruitmentswitch architecture: split
Implementation involves a split synthetic zinc-finger transcription factor whose activity is restored by fusing or otherwise coupling the split components to CRY2 and CIB1 for light-induced association. The available evidence places the system within a modular yeast toolkit in Saccharomyces cerevisiae, but does not provide construct architecture, promoter sequence, illumination parameters, or cofactor requirements.
The supplied evidence is limited to a single 2019 study and a brief mechanistic description. No quantitative performance data, promoter characteristics, dynamic range, wavelength specification, reversibility metrics, or validation outside Saccharomyces cerevisiae are provided here.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The study enables rapid generation and prototyping of optogenetic circuits to control gene expression in Saccharomyces cerevisiae.
This study allows for rapid generation and prototyping of optogenetic circuits to control gene expression in S. cerevisiae.
The study enables rapid generation and prototyping of optogenetic circuits to control gene expression in Saccharomyces cerevisiae.
This study allows for rapid generation and prototyping of optogenetic circuits to control gene expression in S. cerevisiae.
The study enables rapid generation and prototyping of optogenetic circuits to control gene expression in Saccharomyces cerevisiae.
This study allows for rapid generation and prototyping of optogenetic circuits to control gene expression in S. cerevisiae.
The study enables rapid generation and prototyping of optogenetic circuits to control gene expression in Saccharomyces cerevisiae.
This study allows for rapid generation and prototyping of optogenetic circuits to control gene expression in S. cerevisiae.
The study enables rapid generation and prototyping of optogenetic circuits to control gene expression in Saccharomyces cerevisiae.
This study allows for rapid generation and prototyping of optogenetic circuits to control gene expression in S. cerevisiae.
The study enables rapid generation and prototyping of optogenetic circuits to control gene expression in Saccharomyces cerevisiae.
This study allows for rapid generation and prototyping of optogenetic circuits to control gene expression in S. cerevisiae.
The study enables rapid generation and prototyping of optogenetic circuits to control gene expression in Saccharomyces cerevisiae.
This study allows for rapid generation and prototyping of optogenetic circuits to control gene expression in S. cerevisiae.
The study enables rapid generation and prototyping of optogenetic circuits to control gene expression in Saccharomyces cerevisiae.
This study allows for rapid generation and prototyping of optogenetic circuits to control gene expression in S. cerevisiae.
The study enables rapid generation and prototyping of optogenetic circuits to control gene expression in Saccharomyces cerevisiae.
This study allows for rapid generation and prototyping of optogenetic circuits to control gene expression in S. cerevisiae.
The study enables rapid generation and prototyping of optogenetic circuits to control gene expression in Saccharomyces cerevisiae.
This study allows for rapid generation and prototyping of optogenetic circuits to control gene expression in S. cerevisiae.
The study develops an optogenetic system for gene expression control integrated with an existing yeast toolkit for rapid modular assembly of light-controlled circuits in Saccharomyces cerevisiae.
Here we develop an optogenetic system for gene expression control integrated with an existing yeast toolkit allowing for rapid, modular assembly of light-controlled circuits in the important chassis organism Saccharomyces cerevisiae.
The study develops an optogenetic system for gene expression control integrated with an existing yeast toolkit for rapid modular assembly of light-controlled circuits in Saccharomyces cerevisiae.
Here we develop an optogenetic system for gene expression control integrated with an existing yeast toolkit allowing for rapid, modular assembly of light-controlled circuits in the important chassis organism Saccharomyces cerevisiae.
The study develops an optogenetic system for gene expression control integrated with an existing yeast toolkit for rapid modular assembly of light-controlled circuits in Saccharomyces cerevisiae.
Here we develop an optogenetic system for gene expression control integrated with an existing yeast toolkit allowing for rapid, modular assembly of light-controlled circuits in the important chassis organism Saccharomyces cerevisiae.
The study develops an optogenetic system for gene expression control integrated with an existing yeast toolkit for rapid modular assembly of light-controlled circuits in Saccharomyces cerevisiae.
Here we develop an optogenetic system for gene expression control integrated with an existing yeast toolkit allowing for rapid, modular assembly of light-controlled circuits in the important chassis organism Saccharomyces cerevisiae.
The study develops an optogenetic system for gene expression control integrated with an existing yeast toolkit for rapid modular assembly of light-controlled circuits in Saccharomyces cerevisiae.
Here we develop an optogenetic system for gene expression control integrated with an existing yeast toolkit allowing for rapid, modular assembly of light-controlled circuits in the important chassis organism Saccharomyces cerevisiae.
The study develops an optogenetic system for gene expression control integrated with an existing yeast toolkit for rapid modular assembly of light-controlled circuits in Saccharomyces cerevisiae.
Here we develop an optogenetic system for gene expression control integrated with an existing yeast toolkit allowing for rapid, modular assembly of light-controlled circuits in the important chassis organism Saccharomyces cerevisiae.
The study develops an optogenetic system for gene expression control integrated with an existing yeast toolkit for rapid modular assembly of light-controlled circuits in Saccharomyces cerevisiae.
Here we develop an optogenetic system for gene expression control integrated with an existing yeast toolkit allowing for rapid, modular assembly of light-controlled circuits in the important chassis organism Saccharomyces cerevisiae.
The study develops an optogenetic system for gene expression control integrated with an existing yeast toolkit for rapid modular assembly of light-controlled circuits in Saccharomyces cerevisiae.
Here we develop an optogenetic system for gene expression control integrated with an existing yeast toolkit allowing for rapid, modular assembly of light-controlled circuits in the important chassis organism Saccharomyces cerevisiae.
The study develops an optogenetic system for gene expression control integrated with an existing yeast toolkit for rapid modular assembly of light-controlled circuits in Saccharomyces cerevisiae.
Here we develop an optogenetic system for gene expression control integrated with an existing yeast toolkit allowing for rapid, modular assembly of light-controlled circuits in the important chassis organism Saccharomyces cerevisiae.
The study develops an optogenetic system for gene expression control integrated with an existing yeast toolkit for rapid modular assembly of light-controlled circuits in Saccharomyces cerevisiae.
Here we develop an optogenetic system for gene expression control integrated with an existing yeast toolkit allowing for rapid, modular assembly of light-controlled circuits in the important chassis organism Saccharomyces cerevisiae.
Activity of a split synthetic zinc-finger transcription factor is reconstituted using CRY2- and CIB1-mediated light-induced dimerization.
We reconstitute activity of a split synthetic zinc-finger transcription factor (TF) using light-induced dimerization mediated by the proteins CRY2 and CIB1.
Activity of a split synthetic zinc-finger transcription factor is reconstituted using CRY2- and CIB1-mediated light-induced dimerization.
We reconstitute activity of a split synthetic zinc-finger transcription factor (TF) using light-induced dimerization mediated by the proteins CRY2 and CIB1.
Activity of a split synthetic zinc-finger transcription factor is reconstituted using CRY2- and CIB1-mediated light-induced dimerization.
We reconstitute activity of a split synthetic zinc-finger transcription factor (TF) using light-induced dimerization mediated by the proteins CRY2 and CIB1.
Activity of a split synthetic zinc-finger transcription factor is reconstituted using CRY2- and CIB1-mediated light-induced dimerization.
We reconstitute activity of a split synthetic zinc-finger transcription factor (TF) using light-induced dimerization mediated by the proteins CRY2 and CIB1.
Activity of a split synthetic zinc-finger transcription factor is reconstituted using CRY2- and CIB1-mediated light-induced dimerization.
We reconstitute activity of a split synthetic zinc-finger transcription factor (TF) using light-induced dimerization mediated by the proteins CRY2 and CIB1.
Activity of a split synthetic zinc-finger transcription factor is reconstituted using CRY2- and CIB1-mediated light-induced dimerization.
We reconstitute activity of a split synthetic zinc-finger transcription factor (TF) using light-induced dimerization mediated by the proteins CRY2 and CIB1.
Activity of a split synthetic zinc-finger transcription factor is reconstituted using CRY2- and CIB1-mediated light-induced dimerization.
We reconstitute activity of a split synthetic zinc-finger transcription factor (TF) using light-induced dimerization mediated by the proteins CRY2 and CIB1.
Activity of a split synthetic zinc-finger transcription factor is reconstituted using CRY2- and CIB1-mediated light-induced dimerization.
We reconstitute activity of a split synthetic zinc-finger transcription factor (TF) using light-induced dimerization mediated by the proteins CRY2 and CIB1.
Activity of a split synthetic zinc-finger transcription factor is reconstituted using CRY2- and CIB1-mediated light-induced dimerization.
We reconstitute activity of a split synthetic zinc-finger transcription factor (TF) using light-induced dimerization mediated by the proteins CRY2 and CIB1.
Activity of a split synthetic zinc-finger transcription factor is reconstituted using CRY2- and CIB1-mediated light-induced dimerization.
We reconstitute activity of a split synthetic zinc-finger transcription factor (TF) using light-induced dimerization mediated by the proteins CRY2 and CIB1.
Activity of a split synthetic zinc-finger transcription factor is reconstituted using CRY2- and CIB1-mediated light-induced dimerization.
We reconstitute activity of a split synthetic zinc-finger transcription factor (TF) using light-induced dimerization mediated by the proteins CRY2 and CIB1.
Activity of a split synthetic zinc-finger transcription factor is reconstituted using CRY2- and CIB1-mediated light-induced dimerization.
We reconstitute activity of a split synthetic zinc-finger transcription factor (TF) using light-induced dimerization mediated by the proteins CRY2 and CIB1.
Activity of a split synthetic zinc-finger transcription factor is reconstituted using CRY2- and CIB1-mediated light-induced dimerization.
We reconstitute activity of a split synthetic zinc-finger transcription factor (TF) using light-induced dimerization mediated by the proteins CRY2 and CIB1.
Activity of a split synthetic zinc-finger transcription factor is reconstituted using CRY2- and CIB1-mediated light-induced dimerization.
We reconstitute activity of a split synthetic zinc-finger transcription factor (TF) using light-induced dimerization mediated by the proteins CRY2 and CIB1.
Activity of a split synthetic zinc-finger transcription factor is reconstituted using CRY2- and CIB1-mediated light-induced dimerization.
We reconstitute activity of a split synthetic zinc-finger transcription factor (TF) using light-induced dimerization mediated by the proteins CRY2 and CIB1.
Activity of a split synthetic zinc-finger transcription factor is reconstituted using CRY2- and CIB1-mediated light-induced dimerization.
We reconstitute activity of a split synthetic zinc-finger transcription factor (TF) using light-induced dimerization mediated by the proteins CRY2 and CIB1.
Activity of a split synthetic zinc-finger transcription factor is reconstituted using CRY2- and CIB1-mediated light-induced dimerization.
We reconstitute activity of a split synthetic zinc-finger transcription factor (TF) using light-induced dimerization mediated by the proteins CRY2 and CIB1.
Using the split transcription factor and a synthetic promoter, light intensity and duty cycle can modulate gene expression over the range currently available from natural yeast promoters.
Utilizing this TF and a synthetic promoter we demonstrate that light intensity and duty cycle can be used to modulate gene expression over the range currently available from natural yeast promoters.
Using the split transcription factor and a synthetic promoter, light intensity and duty cycle can modulate gene expression over the range currently available from natural yeast promoters.
Utilizing this TF and a synthetic promoter we demonstrate that light intensity and duty cycle can be used to modulate gene expression over the range currently available from natural yeast promoters.
Using the split transcription factor and a synthetic promoter, light intensity and duty cycle can modulate gene expression over the range currently available from natural yeast promoters.
Utilizing this TF and a synthetic promoter we demonstrate that light intensity and duty cycle can be used to modulate gene expression over the range currently available from natural yeast promoters.
Using the split transcription factor and a synthetic promoter, light intensity and duty cycle can modulate gene expression over the range currently available from natural yeast promoters.
Utilizing this TF and a synthetic promoter we demonstrate that light intensity and duty cycle can be used to modulate gene expression over the range currently available from natural yeast promoters.
Using the split transcription factor and a synthetic promoter, light intensity and duty cycle can modulate gene expression over the range currently available from natural yeast promoters.
Utilizing this TF and a synthetic promoter we demonstrate that light intensity and duty cycle can be used to modulate gene expression over the range currently available from natural yeast promoters.
Using the split transcription factor and a synthetic promoter, light intensity and duty cycle can modulate gene expression over the range currently available from natural yeast promoters.
Utilizing this TF and a synthetic promoter we demonstrate that light intensity and duty cycle can be used to modulate gene expression over the range currently available from natural yeast promoters.
Using the split transcription factor and a synthetic promoter, light intensity and duty cycle can modulate gene expression over the range currently available from natural yeast promoters.
Utilizing this TF and a synthetic promoter we demonstrate that light intensity and duty cycle can be used to modulate gene expression over the range currently available from natural yeast promoters.
Using the split transcription factor and a synthetic promoter, light intensity and duty cycle can modulate gene expression over the range currently available from natural yeast promoters.
Utilizing this TF and a synthetic promoter we demonstrate that light intensity and duty cycle can be used to modulate gene expression over the range currently available from natural yeast promoters.
Using the split transcription factor and a synthetic promoter, light intensity and duty cycle can modulate gene expression over the range currently available from natural yeast promoters.
Utilizing this TF and a synthetic promoter we demonstrate that light intensity and duty cycle can be used to modulate gene expression over the range currently available from natural yeast promoters.
Using the split transcription factor and a synthetic promoter, light intensity and duty cycle can modulate gene expression over the range currently available from natural yeast promoters.
Utilizing this TF and a synthetic promoter we demonstrate that light intensity and duty cycle can be used to modulate gene expression over the range currently available from natural yeast promoters.
Using the split transcription factor and a synthetic promoter, light intensity and duty cycle can modulate gene expression over the range currently available from natural yeast promoters.
Utilizing this TF and a synthetic promoter we demonstrate that light intensity and duty cycle can be used to modulate gene expression over the range currently available from natural yeast promoters.
Using the split transcription factor and a synthetic promoter, light intensity and duty cycle can modulate gene expression over the range currently available from natural yeast promoters.
Utilizing this TF and a synthetic promoter we demonstrate that light intensity and duty cycle can be used to modulate gene expression over the range currently available from natural yeast promoters.
Using the split transcription factor and a synthetic promoter, light intensity and duty cycle can modulate gene expression over the range currently available from natural yeast promoters.
Utilizing this TF and a synthetic promoter we demonstrate that light intensity and duty cycle can be used to modulate gene expression over the range currently available from natural yeast promoters.
Using the split transcription factor and a synthetic promoter, light intensity and duty cycle can modulate gene expression over the range currently available from natural yeast promoters.
Utilizing this TF and a synthetic promoter we demonstrate that light intensity and duty cycle can be used to modulate gene expression over the range currently available from natural yeast promoters.
Using the split transcription factor and a synthetic promoter, light intensity and duty cycle can modulate gene expression over the range currently available from natural yeast promoters.
Utilizing this TF and a synthetic promoter we demonstrate that light intensity and duty cycle can be used to modulate gene expression over the range currently available from natural yeast promoters.
Using the split transcription factor and a synthetic promoter, light intensity and duty cycle can modulate gene expression over the range currently available from natural yeast promoters.
Utilizing this TF and a synthetic promoter we demonstrate that light intensity and duty cycle can be used to modulate gene expression over the range currently available from natural yeast promoters.
Using the split transcription factor and a synthetic promoter, light intensity and duty cycle can modulate gene expression over the range currently available from natural yeast promoters.
Utilizing this TF and a synthetic promoter we demonstrate that light intensity and duty cycle can be used to modulate gene expression over the range currently available from natural yeast promoters.
Approval Evidence
1 source2 linked approval claimsfirst-pass slug split-synthetic-zinc-finger-transcription-factor
We reconstitute activity of a split synthetic zinc-finger transcription factor (TF) using light-induced dimerization mediated by the proteins CRY2 and CIB1.
Source:
mechanismsupports
Activity of a split synthetic zinc-finger transcription factor is reconstituted using CRY2- and CIB1-mediated light-induced dimerization.
We reconstitute activity of a split synthetic zinc-finger transcription factor (TF) using light-induced dimerization mediated by the proteins CRY2 and CIB1.
Source:
modulation capabilitysupports
Using the split transcription factor and a synthetic promoter, light intensity and duty cycle can modulate gene expression over the range currently available from natural yeast promoters.
Utilizing this TF and a synthetic promoter we demonstrate that light intensity and duty cycle can be used to modulate gene expression over the range currently available from natural yeast promoters.
Source:
Comparisons
Source-backed strengths
The reported strength is light-dependent reconstitution of a split synthetic zinc-finger transcription factor via the CRY2/CIB1 interaction. The system was also integrated with an existing yeast toolkit for rapid modular assembly of light-controlled circuits in S. cerevisiae.
Compared with CRY2-CIB1 light-inducible transcription system
split synthetic zinc-finger transcription factor and CRY2-CIB1 light-inducible transcription system address a similar problem space because they share transcription.
Shared frame: same top-level item type; shared target processes: transcription; shared mechanisms: light-induced heterodimerization; same primary input modality: light
Compared with LITEs (Light-inducible transcriptional effectors)
split synthetic zinc-finger transcription factor and LITEs (Light-inducible transcriptional effectors) address a similar problem space because they share transcription.
Shared frame: same top-level item type; shared target processes: transcription; shared mechanisms: heterodimerization; same primary input modality: light
Compared with mOptoT7
split synthetic zinc-finger transcription factor and mOptoT7 address a similar problem space because they share transcription.
Shared frame: same top-level item type; shared target processes: transcription; shared mechanisms: light-induced heterodimerization; same primary input modality: light
Relative tradeoffs: appears more independently replicated; looks easier to implement in practice.
Ranked Citations
- 1.