Source 1primary paper2022
Toolkit/split-TurboID
split-TurboID
Taxonomy: Mechanism Branch / Component. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
split-TurboID is a split proximity-labeling enzyme used in the Light Activated BioID (LAB) system, where its two halves are fused to the photodimeric proteins CRY2 and CIB1. Blue light induces CRY2–CIB1 association, reconstitutes split-TurboID, and triggers proximity-dependent biotinylation.
Usefulness & Problems
Why this is useful
In the LAB configuration, split-TurboID enables optically controlled proximity labeling with temporal gating by blue light. Source literature reports that LAB mapped E-cadherin-binding partners with higher accuracy and significantly fewer false positives than full TurboID.
Source:
Here, we present a light-activated proximity labeling technology for mapping protein-protein interactions at the cell membrane with high accuracy and precision.
Source:
We validate LAB in different cell types and demonstrate that it can identify known binding partners of proteins while reducing background labeling and false positives.
Source:
Here, we present a high spatial and temporal resolution technology that can be activated on demand using light, for high accuracy proximity labeling.
Problem solved
This tool helps address the problem of reducing background and false-positive labeling during proximity-dependent biotinylation experiments. By coupling split-enzyme reconstitution to light-induced CRY2–CIB1 dimerization, labeling is restricted to illuminated periods.
Source:
We validate LAB in different cell types and demonstrate that it can identify known binding partners of proteins while reducing background labeling and false positives.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Component: A low-level protein part used inside a larger architecture that realizes a mechanism.
Mechanisms
Heterodimerizationlight-induced enzyme reconstitutionproximity-dependent biotinylationreversible optical switchingTechniques
No technique tags yet.
Target processes
No target processes tagged yet.
Input: Light
Implementation Constraints
In LAB, the two halves of split-TurboID are genetically fused to CRY2 and CIB1, respectively. Activation requires blue light illumination to drive CRY2–CIB1 dimerization and reconstitute the labeling enzyme; the supplied evidence does not provide additional construct, expression, or cofactor details.
The supplied evidence is limited to the LAB implementation and does not establish performance across multiple targets, cell types, or organisms. Quantitative details such as labeling kinetics, biotin requirements, dynamic range, and residual dark-state activity are not provided in the supplied evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Observations
successMammalian Cell Linemechanistic demo
Inferred from claim claim_3 during normalization. Upon blue light illumination, CRY2 and CIB1 dimerize, reconstitute split-TurboID, and initiate biotinylation. Derived from claim claim_3. Quoted text: upon illumination with blue light, CRY2 and CIB1 dimerize, reconstitute split-TurboID and initiate biotinylation
Source:
successMammalian Cell Linemechanistic demo
Inferred from claim claim_3 during normalization. Upon blue light illumination, CRY2 and CIB1 dimerize, reconstitute split-TurboID, and initiate biotinylation. Derived from claim claim_3. Quoted text: upon illumination with blue light, CRY2 and CIB1 dimerize, reconstitute split-TurboID and initiate biotinylation
Source:
successMammalian Cell Linemechanistic demo
Inferred from claim claim_3 during normalization. Upon blue light illumination, CRY2 and CIB1 dimerize, reconstitute split-TurboID, and initiate biotinylation. Derived from claim claim_3. Quoted text: upon illumination with blue light, CRY2 and CIB1 dimerize, reconstitute split-TurboID and initiate biotinylation
Source:
successMammalian Cell Linemechanistic demo
Inferred from claim claim_3 during normalization. Upon blue light illumination, CRY2 and CIB1 dimerize, reconstitute split-TurboID, and initiate biotinylation. Derived from claim claim_3. Quoted text: upon illumination with blue light, CRY2 and CIB1 dimerize, reconstitute split-TurboID and initiate biotinylation
Source:
successMammalian Cell Linemechanistic demo
Inferred from claim claim_3 during normalization. Upon blue light illumination, CRY2 and CIB1 dimerize, reconstitute split-TurboID, and initiate biotinylation. Derived from claim claim_3. Quoted text: upon illumination with blue light, CRY2 and CIB1 dimerize, reconstitute split-TurboID and initiate biotinylation
Source:
successMammalian Cell Linemechanistic demo
Inferred from claim claim_3 during normalization. Upon blue light illumination, CRY2 and CIB1 dimerize, reconstitute split-TurboID, and initiate biotinylation. Derived from claim claim_3. Quoted text: upon illumination with blue light, CRY2 and CIB1 dimerize, reconstitute split-TurboID and initiate biotinylation
Source:
successMammalian Cell Linemechanistic demo
Inferred from claim claim_3 during normalization. Upon blue light illumination, CRY2 and CIB1 dimerize, reconstitute split-TurboID, and initiate biotinylation. Derived from claim claim_3. Quoted text: upon illumination with blue light, CRY2 and CIB1 dimerize, reconstitute split-TurboID and initiate biotinylation
Source:
Supporting Sources
Source 2primary paper2023Journal of Cell Science
Ranked Claims
LAB maps E-cadherin-binding partners with higher accuracy and significantly fewer false positives than TurboID.
We show that LAB can map E-cadherin-binding partners with higher accuracy and significantly fewer false positives than TurboID.
LAB maps E-cadherin-binding partners with higher accuracy and significantly fewer false positives than TurboID.
We show that LAB can map E-cadherin-binding partners with higher accuracy and significantly fewer false positives than TurboID.
LAB maps E-cadherin-binding partners with higher accuracy and significantly fewer false positives than TurboID.
We show that LAB can map E-cadherin-binding partners with higher accuracy and significantly fewer false positives than TurboID.
LAB maps E-cadherin-binding partners with higher accuracy and significantly fewer false positives than TurboID.
We show that LAB can map E-cadherin-binding partners with higher accuracy and significantly fewer false positives than TurboID.
LAB maps E-cadherin-binding partners with higher accuracy and significantly fewer false positives than TurboID.
We show that LAB can map E-cadherin-binding partners with higher accuracy and significantly fewer false positives than TurboID.
LAB maps E-cadherin-binding partners with higher accuracy and significantly fewer false positives than TurboID.
We show that LAB can map E-cadherin-binding partners with higher accuracy and significantly fewer false positives than TurboID.
LAB fuses the two halves of split-TurboID to the photodimeric proteins CRY2 and CIB1.
Our technology, called light-activated BioID (LAB), fuses the two halves of the split-TurboID proximity labeling enzyme to the photodimeric proteins CRY2 and CIB1.
LAB fuses the two halves of split-TurboID to the photodimeric proteins CRY2 and CIB1.
Our technology, called light-activated BioID (LAB), fuses the two halves of the split-TurboID proximity labeling enzyme to the photodimeric proteins CRY2 and CIB1.
LAB fuses the two halves of split-TurboID to the photodimeric proteins CRY2 and CIB1.
Our technology, called light-activated BioID (LAB), fuses the two halves of the split-TurboID proximity labeling enzyme to the photodimeric proteins CRY2 and CIB1.
LAB fuses the two halves of split-TurboID to the photodimeric proteins CRY2 and CIB1.
Our technology, called light-activated BioID (LAB), fuses the two halves of the split-TurboID proximity labeling enzyme to the photodimeric proteins CRY2 and CIB1.
LAB fuses the two halves of split-TurboID to the photodimeric proteins CRY2 and CIB1.
Our technology, called light-activated BioID (LAB), fuses the two halves of the split-TurboID proximity labeling enzyme to the photodimeric proteins CRY2 and CIB1.
LAB fuses the two halves of split-TurboID to the photodimeric proteins CRY2 and CIB1.
Our technology, called light-activated BioID (LAB), fuses the two halves of the split-TurboID proximity labeling enzyme to the photodimeric proteins CRY2 and CIB1.
LAB fuses the two halves of split-TurboID to the photodimeric proteins CRY2 and CIB1.
Our technology, called light-activated BioID (LAB), fuses the two halves of the split-TurboID proximity labeling enzyme to the photodimeric proteins CRY2 and CIB1.
Upon blue light illumination, CRY2 and CIB1 dimerize, reconstitute split-TurboID, and initiate biotinylation.
upon illumination with blue light, CRY2 and CIB1 dimerize, reconstitute split-TurboID and initiate biotinylation
Upon blue light illumination, CRY2 and CIB1 dimerize, reconstitute split-TurboID, and initiate biotinylation.
upon illumination with blue light, CRY2 and CIB1 dimerize, reconstitute split-TurboID and initiate biotinylation
Upon blue light illumination, CRY2 and CIB1 dimerize, reconstitute split-TurboID, and initiate biotinylation.
upon illumination with blue light, CRY2 and CIB1 dimerize, reconstitute split-TurboID and initiate biotinylation
Upon blue light illumination, CRY2 and CIB1 dimerize, reconstitute split-TurboID, and initiate biotinylation.
upon illumination with blue light, CRY2 and CIB1 dimerize, reconstitute split-TurboID and initiate biotinylation
Upon blue light illumination, CRY2 and CIB1 dimerize, reconstitute split-TurboID, and initiate biotinylation.
upon illumination with blue light, CRY2 and CIB1 dimerize, reconstitute split-TurboID and initiate biotinylation
Upon blue light illumination, CRY2 and CIB1 dimerize, reconstitute split-TurboID, and initiate biotinylation.
upon illumination with blue light, CRY2 and CIB1 dimerize, reconstitute split-TurboID and initiate biotinylation
Upon blue light illumination, CRY2 and CIB1 dimerize, reconstitute split-TurboID, and initiate biotinylation.
upon illumination with blue light, CRY2 and CIB1 dimerize, reconstitute split-TurboID and initiate biotinylation
Turning off the light causes CRY2 and CIB1 to dissociate and halts biotinylation.
Turning off the light leads to the dissociation of CRY2 and CIB1 and halts biotinylation.
Turning off the light causes CRY2 and CIB1 to dissociate and halts biotinylation.
Turning off the light leads to the dissociation of CRY2 and CIB1 and halts biotinylation.
Turning off the light causes CRY2 and CIB1 to dissociate and halts biotinylation.
Turning off the light leads to the dissociation of CRY2 and CIB1 and halts biotinylation.
Turning off the light causes CRY2 and CIB1 to dissociate and halts biotinylation.
Turning off the light leads to the dissociation of CRY2 and CIB1 and halts biotinylation.
Turning off the light causes CRY2 and CIB1 to dissociate and halts biotinylation.
Turning off the light leads to the dissociation of CRY2 and CIB1 and halts biotinylation.
Turning off the light causes CRY2 and CIB1 to dissociate and halts biotinylation.
Turning off the light leads to the dissociation of CRY2 and CIB1 and halts biotinylation.
Turning off the light causes CRY2 and CIB1 to dissociate and halts biotinylation.
Turning off the light leads to the dissociation of CRY2 and CIB1 and halts biotinylation.
Light-activated BioID is a light-activated proximity labeling technology for mapping protein-protein interactions at the cell membrane with high accuracy and precision.
Here, we present a light-activated proximity labeling technology for mapping protein-protein interactions at the cell membrane with high accuracy and precision.
Light-activated BioID is a light-activated proximity labeling technology for mapping protein-protein interactions at the cell membrane with high accuracy and precision.
Here, we present a light-activated proximity labeling technology for mapping protein-protein interactions at the cell membrane with high accuracy and precision.
Light-activated BioID is a light-activated proximity labeling technology for mapping protein-protein interactions at the cell membrane with high accuracy and precision.
Here, we present a light-activated proximity labeling technology for mapping protein-protein interactions at the cell membrane with high accuracy and precision.
Light-activated BioID is a light-activated proximity labeling technology for mapping protein-protein interactions at the cell membrane with high accuracy and precision.
Here, we present a light-activated proximity labeling technology for mapping protein-protein interactions at the cell membrane with high accuracy and precision.
Light-activated BioID is a light-activated proximity labeling technology for mapping protein-protein interactions at the cell membrane with high accuracy and precision.
Here, we present a light-activated proximity labeling technology for mapping protein-protein interactions at the cell membrane with high accuracy and precision.
Light-activated BioID is a light-activated proximity labeling technology for mapping protein-protein interactions at the cell membrane with high accuracy and precision.
Here, we present a light-activated proximity labeling technology for mapping protein-protein interactions at the cell membrane with high accuracy and precision.
LAB can identify known binding partners of proteins while reducing background labeling and false positives.
We validate LAB in different cell types and demonstrate that it can identify known binding partners of proteins while reducing background labeling and false positives.
LAB can identify known binding partners of proteins while reducing background labeling and false positives.
We validate LAB in different cell types and demonstrate that it can identify known binding partners of proteins while reducing background labeling and false positives.
LAB can identify known binding partners of proteins while reducing background labeling and false positives.
We validate LAB in different cell types and demonstrate that it can identify known binding partners of proteins while reducing background labeling and false positives.
LAB can identify known binding partners of proteins while reducing background labeling and false positives.
We validate LAB in different cell types and demonstrate that it can identify known binding partners of proteins while reducing background labeling and false positives.
LAB can identify known binding partners of proteins while reducing background labeling and false positives.
We validate LAB in different cell types and demonstrate that it can identify known binding partners of proteins while reducing background labeling and false positives.
LAB can identify known binding partners of proteins while reducing background labeling and false positives.
We validate LAB in different cell types and demonstrate that it can identify known binding partners of proteins while reducing background labeling and false positives.
LAB can identify known binding partners of proteins while reducing background labeling and false positives.
We validate LAB in different cell types and demonstrate that it can identify known binding partners of proteins while reducing background labeling and false positives.
LAB is generated by fusing split-TurboID halves to the photodimeric proteins CRY2 and CIB1.
Our system, called Light Activated BioID (LAB), is generated by fusing the two halves of the split-TurboID proximity labeling enzyme to the photodimeric proteins CRY2 and CIB1.
LAB is generated by fusing split-TurboID halves to the photodimeric proteins CRY2 and CIB1.
Our system, called Light Activated BioID (LAB), is generated by fusing the two halves of the split-TurboID proximity labeling enzyme to the photodimeric proteins CRY2 and CIB1.
LAB is generated by fusing split-TurboID halves to the photodimeric proteins CRY2 and CIB1.
Our system, called Light Activated BioID (LAB), is generated by fusing the two halves of the split-TurboID proximity labeling enzyme to the photodimeric proteins CRY2 and CIB1.
LAB is generated by fusing split-TurboID halves to the photodimeric proteins CRY2 and CIB1.
Our system, called Light Activated BioID (LAB), is generated by fusing the two halves of the split-TurboID proximity labeling enzyme to the photodimeric proteins CRY2 and CIB1.
LAB is generated by fusing split-TurboID halves to the photodimeric proteins CRY2 and CIB1.
Our system, called Light Activated BioID (LAB), is generated by fusing the two halves of the split-TurboID proximity labeling enzyme to the photodimeric proteins CRY2 and CIB1.
LAB is generated by fusing split-TurboID halves to the photodimeric proteins CRY2 and CIB1.
Our system, called Light Activated BioID (LAB), is generated by fusing the two halves of the split-TurboID proximity labeling enzyme to the photodimeric proteins CRY2 and CIB1.
LAB is generated by fusing split-TurboID halves to the photodimeric proteins CRY2 and CIB1.
Our system, called Light Activated BioID (LAB), is generated by fusing the two halves of the split-TurboID proximity labeling enzyme to the photodimeric proteins CRY2 and CIB1.
Turning off light halts the biotinylation reaction in the LAB system.
Turning off the light halts the biotinylation reaction.
Turning off light halts the biotinylation reaction in the LAB system.
Turning off the light halts the biotinylation reaction.
Turning off light halts the biotinylation reaction in the LAB system.
Turning off the light halts the biotinylation reaction.
Turning off light halts the biotinylation reaction in the LAB system.
Turning off the light halts the biotinylation reaction.
Turning off light halts the biotinylation reaction in the LAB system.
Turning off the light halts the biotinylation reaction.
Turning off light halts the biotinylation reaction in the LAB system.
Turning off the light halts the biotinylation reaction.
Turning off light halts the biotinylation reaction in the LAB system.
Turning off the light halts the biotinylation reaction.
Blue light exposure causes CRY2 and CIB1 to dimerize, reconstitute split-TurboID, and biotinylate proximate proteins in the LAB system.
upon exposure to blue light, CRY2 and CIB1 dimerize, reconstitute the split-TurboID enzyme, and biotinylate proximate proteins
Blue light exposure causes CRY2 and CIB1 to dimerize, reconstitute split-TurboID, and biotinylate proximate proteins in the LAB system.
upon exposure to blue light, CRY2 and CIB1 dimerize, reconstitute the split-TurboID enzyme, and biotinylate proximate proteins
Blue light exposure causes CRY2 and CIB1 to dimerize, reconstitute split-TurboID, and biotinylate proximate proteins in the LAB system.
upon exposure to blue light, CRY2 and CIB1 dimerize, reconstitute the split-TurboID enzyme, and biotinylate proximate proteins
Blue light exposure causes CRY2 and CIB1 to dimerize, reconstitute split-TurboID, and biotinylate proximate proteins in the LAB system.
upon exposure to blue light, CRY2 and CIB1 dimerize, reconstitute the split-TurboID enzyme, and biotinylate proximate proteins
Blue light exposure causes CRY2 and CIB1 to dimerize, reconstitute split-TurboID, and biotinylate proximate proteins in the LAB system.
upon exposure to blue light, CRY2 and CIB1 dimerize, reconstitute the split-TurboID enzyme, and biotinylate proximate proteins
Blue light exposure causes CRY2 and CIB1 to dimerize, reconstitute split-TurboID, and biotinylate proximate proteins in the LAB system.
upon exposure to blue light, CRY2 and CIB1 dimerize, reconstitute the split-TurboID enzyme, and biotinylate proximate proteins
Blue light exposure causes CRY2 and CIB1 to dimerize, reconstitute split-TurboID, and biotinylate proximate proteins in the LAB system.
upon exposure to blue light, CRY2 and CIB1 dimerize, reconstitute the split-TurboID enzyme, and biotinylate proximate proteins
LAB is a light-activated proximity labeling technology with high spatial and temporal resolution.
Here, we present a high spatial and temporal resolution technology that can be activated on demand using light, for high accuracy proximity labeling.
LAB is a light-activated proximity labeling technology with high spatial and temporal resolution.
Here, we present a high spatial and temporal resolution technology that can be activated on demand using light, for high accuracy proximity labeling.
LAB is a light-activated proximity labeling technology with high spatial and temporal resolution.
Here, we present a high spatial and temporal resolution technology that can be activated on demand using light, for high accuracy proximity labeling.
LAB is a light-activated proximity labeling technology with high spatial and temporal resolution.
Here, we present a high spatial and temporal resolution technology that can be activated on demand using light, for high accuracy proximity labeling.
LAB is a light-activated proximity labeling technology with high spatial and temporal resolution.
Here, we present a high spatial and temporal resolution technology that can be activated on demand using light, for high accuracy proximity labeling.
LAB is a light-activated proximity labeling technology with high spatial and temporal resolution.
Here, we present a high spatial and temporal resolution technology that can be activated on demand using light, for high accuracy proximity labeling.
LAB is a light-activated proximity labeling technology with high spatial and temporal resolution.
Here, we present a high spatial and temporal resolution technology that can be activated on demand using light, for high accuracy proximity labeling.
Approval Evidence
2 sources4 linked approval claimsfirst-pass slug split-turboid
fuses the two halves of the split-TurboID proximity labeling enzyme
Source:
Our system, called Light Activated BioID (LAB), is generated by fusing the two halves of the split-TurboID proximity labeling enzyme to the photodimeric proteins CRY2 and CIB1.
Source:
construct architecturesupports
LAB fuses the two halves of split-TurboID to the photodimeric proteins CRY2 and CIB1.
Our technology, called light-activated BioID (LAB), fuses the two halves of the split-TurboID proximity labeling enzyme to the photodimeric proteins CRY2 and CIB1.
Source:
mechanism of actionsupports
Upon blue light illumination, CRY2 and CIB1 dimerize, reconstitute split-TurboID, and initiate biotinylation.
upon illumination with blue light, CRY2 and CIB1 dimerize, reconstitute split-TurboID and initiate biotinylation
Source:
compositionsupports
LAB is generated by fusing split-TurboID halves to the photodimeric proteins CRY2 and CIB1.
Our system, called Light Activated BioID (LAB), is generated by fusing the two halves of the split-TurboID proximity labeling enzyme to the photodimeric proteins CRY2 and CIB1.
Source:
mechanismsupports
Blue light exposure causes CRY2 and CIB1 to dimerize, reconstitute split-TurboID, and biotinylate proximate proteins in the LAB system.
upon exposure to blue light, CRY2 and CIB1 dimerize, reconstitute the split-TurboID enzyme, and biotinylate proximate proteins
Source:
Comparisons
Source-backed strengths
The reported strength of split-TurboID in LAB is light-dependent activation through CRY2–CIB1-mediated enzyme reconstitution. In the cited study, this implementation produced higher-accuracy mapping of E-cadherin-binding partners and significantly fewer false positives than TurboID.
Ranked Citations
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