Source 1primary paper2019Communications Biology
Toolkit/SpyTag/SpyCatcher interaction
SpyTag/SpyCatcher interaction
Also known as: SpyTag/SpyCatcher
Taxonomy: Mechanism Branch / Component. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
SpyTag/SpyCatcher interaction is a protein-domain interaction used as an assistance strategy to improve split fluorescent proteins. In the cited 2019 Communications Biology study, it was demonstrated alongside directed evolution as an approach for improving split sfCherry-based reporters.
Usefulness & Problems
Why this is useful
The available evidence indicates that SpyTag/SpyCatcher is useful as an auxiliary interaction module for improving split fluorescent protein performance. In the cited work, this improvement context was linked to split sfCherry3 variants that facilitated endogenous protein tagging by gene editing and supported synapse visualization applications.
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for multiplexed visualization of neuronal synapses in living C. elegans
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facilitating the tagging of endogenous proteins by gene editing
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Based on sfCherry3, we have further developed a new red-colored trans-synaptic marker called Neuroligin-1 sfCherry3 Linker Across Synaptic Partners (NLG-1 CLASP)
Problem solved
This interaction was used to address the challenge of improving split fluorescent proteins. The supplied evidence does not specify quantitative deficiencies corrected by SpyTag/SpyCatcher beyond its role as an assistance strategy in the split sfCherry optimization workflow.
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for multiplexed visualization of neuronal synapses in living C. elegans
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facilitating the tagging of endogenous proteins by gene editing
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Based on sfCherry3, we have further developed a new red-colored trans-synaptic marker called Neuroligin-1 sfCherry3 Linker Across Synaptic Partners (NLG-1 CLASP)
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Component: A low-level protein part used inside a larger architecture that realizes a mechanism.
Mechanisms
protein-protein interactionTechniques
Directed EvolutionTarget processes
No target processes tagged yet.
Implementation Constraints
The evidence only states that improvement was achieved through assistance by SpyTag/SpyCatcher interaction in a split fluorescent protein context. No construct architecture, linker design, expression system, cofactor requirement, or delivery method is described in the supplied text.
The supplied evidence does not report mechanistic detail beyond protein-domain interaction, nor does it provide quantitative performance metrics attributable specifically to SpyTag/SpyCatcher. Independent replication, organismal breadth, and implementation constraints are not documented in the provided material.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
NLG-1 CLASP enables multiplexed visualization of neuronal synapses in living C. elegans.
for multiplexed visualization of neuronal synapses in living C. elegans
NLG-1 CLASP enables multiplexed visualization of neuronal synapses in living C. elegans.
for multiplexed visualization of neuronal synapses in living C. elegans
NLG-1 CLASP enables multiplexed visualization of neuronal synapses in living C. elegans.
for multiplexed visualization of neuronal synapses in living C. elegans
NLG-1 CLASP enables multiplexed visualization of neuronal synapses in living C. elegans.
for multiplexed visualization of neuronal synapses in living C. elegans
NLG-1 CLASP enables multiplexed visualization of neuronal synapses in living C. elegans.
for multiplexed visualization of neuronal synapses in living C. elegans
NLG-1 CLASP enables multiplexed visualization of neuronal synapses in living C. elegans.
for multiplexed visualization of neuronal synapses in living C. elegans
NLG-1 CLASP enables multiplexed visualization of neuronal synapses in living C. elegans.
for multiplexed visualization of neuronal synapses in living C. elegans
The split sfCherry3 variants facilitate tagging of endogenous proteins by gene editing.
facilitating the tagging of endogenous proteins by gene editing
The split sfCherry3 variants facilitate tagging of endogenous proteins by gene editing.
facilitating the tagging of endogenous proteins by gene editing
The split sfCherry3 variants facilitate tagging of endogenous proteins by gene editing.
facilitating the tagging of endogenous proteins by gene editing
The split sfCherry3 variants facilitate tagging of endogenous proteins by gene editing.
facilitating the tagging of endogenous proteins by gene editing
The split sfCherry3 variants facilitate tagging of endogenous proteins by gene editing.
facilitating the tagging of endogenous proteins by gene editing
The split sfCherry3 variants facilitate tagging of endogenous proteins by gene editing.
facilitating the tagging of endogenous proteins by gene editing
The split sfCherry3 variants facilitate tagging of endogenous proteins by gene editing.
facilitating the tagging of endogenous proteins by gene editing
SpyTag/SpyCatcher interaction and directed evolution were demonstrated as two approaches to improve split fluorescent proteins.
we have demonstrated two approaches to improve split FPs: assistance through SpyTag/SpyCatcher interaction and directed evolution
SpyTag/SpyCatcher interaction and directed evolution were demonstrated as two approaches to improve split fluorescent proteins.
we have demonstrated two approaches to improve split FPs: assistance through SpyTag/SpyCatcher interaction and directed evolution
SpyTag/SpyCatcher interaction and directed evolution were demonstrated as two approaches to improve split fluorescent proteins.
we have demonstrated two approaches to improve split FPs: assistance through SpyTag/SpyCatcher interaction and directed evolution
SpyTag/SpyCatcher interaction and directed evolution were demonstrated as two approaches to improve split fluorescent proteins.
we have demonstrated two approaches to improve split FPs: assistance through SpyTag/SpyCatcher interaction and directed evolution
SpyTag/SpyCatcher interaction and directed evolution were demonstrated as two approaches to improve split fluorescent proteins.
we have demonstrated two approaches to improve split FPs: assistance through SpyTag/SpyCatcher interaction and directed evolution
SpyTag/SpyCatcher interaction and directed evolution were demonstrated as two approaches to improve split fluorescent proteins.
we have demonstrated two approaches to improve split FPs: assistance through SpyTag/SpyCatcher interaction and directed evolution
SpyTag/SpyCatcher interaction and directed evolution were demonstrated as two approaches to improve split fluorescent proteins.
we have demonstrated two approaches to improve split FPs: assistance through SpyTag/SpyCatcher interaction and directed evolution
Directed evolution yielded two split sfCherry3 variants with substantially enhanced overall brightness.
The latter has yielded two split sfCherry3 variants with substantially enhanced overall brightness
Directed evolution yielded two split sfCherry3 variants with substantially enhanced overall brightness.
The latter has yielded two split sfCherry3 variants with substantially enhanced overall brightness
Directed evolution yielded two split sfCherry3 variants with substantially enhanced overall brightness.
The latter has yielded two split sfCherry3 variants with substantially enhanced overall brightness
Directed evolution yielded two split sfCherry3 variants with substantially enhanced overall brightness.
The latter has yielded two split sfCherry3 variants with substantially enhanced overall brightness
Directed evolution yielded two split sfCherry3 variants with substantially enhanced overall brightness.
The latter has yielded two split sfCherry3 variants with substantially enhanced overall brightness
Directed evolution yielded two split sfCherry3 variants with substantially enhanced overall brightness.
The latter has yielded two split sfCherry3 variants with substantially enhanced overall brightness
Directed evolution yielded two split sfCherry3 variants with substantially enhanced overall brightness.
The latter has yielded two split sfCherry3 variants with substantially enhanced overall brightness
Based on sfCherry3, the authors developed a new red-colored trans-synaptic marker called NLG-1 CLASP.
Based on sfCherry3, we have further developed a new red-colored trans-synaptic marker called Neuroligin-1 sfCherry3 Linker Across Synaptic Partners (NLG-1 CLASP)
Based on sfCherry3, the authors developed a new red-colored trans-synaptic marker called NLG-1 CLASP.
Based on sfCherry3, we have further developed a new red-colored trans-synaptic marker called Neuroligin-1 sfCherry3 Linker Across Synaptic Partners (NLG-1 CLASP)
Based on sfCherry3, the authors developed a new red-colored trans-synaptic marker called NLG-1 CLASP.
Based on sfCherry3, we have further developed a new red-colored trans-synaptic marker called Neuroligin-1 sfCherry3 Linker Across Synaptic Partners (NLG-1 CLASP)
Based on sfCherry3, the authors developed a new red-colored trans-synaptic marker called NLG-1 CLASP.
Based on sfCherry3, we have further developed a new red-colored trans-synaptic marker called Neuroligin-1 sfCherry3 Linker Across Synaptic Partners (NLG-1 CLASP)
Based on sfCherry3, the authors developed a new red-colored trans-synaptic marker called NLG-1 CLASP.
Based on sfCherry3, we have further developed a new red-colored trans-synaptic marker called Neuroligin-1 sfCherry3 Linker Across Synaptic Partners (NLG-1 CLASP)
Based on sfCherry3, the authors developed a new red-colored trans-synaptic marker called NLG-1 CLASP.
Based on sfCherry3, we have further developed a new red-colored trans-synaptic marker called Neuroligin-1 sfCherry3 Linker Across Synaptic Partners (NLG-1 CLASP)
Based on sfCherry3, the authors developed a new red-colored trans-synaptic marker called NLG-1 CLASP.
Based on sfCherry3, we have further developed a new red-colored trans-synaptic marker called Neuroligin-1 sfCherry3 Linker Across Synaptic Partners (NLG-1 CLASP)
Approval Evidence
1 source1 linked approval claimfirst-pass slug spytag-spycatcher-interaction
assistance through SpyTag/SpyCatcher interaction
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method improvementsupports
SpyTag/SpyCatcher interaction and directed evolution were demonstrated as two approaches to improve split fluorescent proteins.
we have demonstrated two approaches to improve split FPs: assistance through SpyTag/SpyCatcher interaction and directed evolution
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Comparisons
Source-backed strengths
A key strength supported by the evidence is that SpyTag/SpyCatcher was experimentally demonstrated as one of two successful approaches used to improve split fluorescent proteins. The same study associated the improved split sfCherry3 system with endogenous protein tagging by gene editing and multiplexed visualization of neuronal synapses in living Caenorhabditis elegans.
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The latter has yielded two split sfCherry3 variants with substantially enhanced overall brightness
Ranked Citations
- 1.