Source 1primary paper2021Applied Physics Letters
Toolkit/stimulated depletion quenching
stimulated depletion quenching
Also known as: SDQ
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Stimulated depletion quenching (SDQ) is a nonlinear optical control method, described as similar to STED, for improving photoactivation selectivity in bidirectional photoswitches. It has been developed and implemented as a photoswitching strategy and applied to the Cph8 optogenetic switch to shift photoequilibrium beyond what is achievable with continuous-wave illumination.
Usefulness & Problems
Why this is useful
SDQ is useful for suppressing spectral cross talk during bidirectional optogenetic switching by increasing selectivity of the photoactivation step. Source literature states that this improved selectivity should translate into improved dynamic range across a broad variety of bidirectional switches.
Source:
Increased selectivity of the photoactivation step via nonlinear optical techniques should translate into an improved dynamic range for a broad variety of bidirectional switches.
Source:
SDQ is employed to enhance the control of Cph8, a photo-reversible phytochrome based optogenetic switch designed to control gene expression in E. Coli bacteria.
Source:
This approach is applied to the phytochrome Cph8 bidirectional optogenetic switch
Source:
Stimulated Depletion Quenching (SDQ), is used to overcome spectral cross-talk by exploiting the molecules' unique dynamic response to ultrashort laser pulses
Problem solved
SDQ addresses the problem that bidirectional photoswitches can have limited selectivity in one photoactivation direction, constraining attainable photoequilibrium under conventional continuous-wave light. The method is specifically presented as a way to mitigate spectral cross talk in optogenetic switching.
Source:
Increased selectivity of the photoactivation step via nonlinear optical techniques should translate into an improved dynamic range for a broad variety of bidirectional switches.
Source:
SDQ is employed to enhance the control of Cph8, a photo-reversible phytochrome based optogenetic switch designed to control gene expression in E. Coli bacteria.
Source:
This approach is applied to the phytochrome Cph8 bidirectional optogenetic switch
Source:
The major challenge is the multiplexed control of several optogenetic components in the presence of significant spectral cross talk.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete method used to build, optimize, or evolve an engineered system.
Mechanisms
nonlinear optical controlphotoactivation selectivity enhancementstimulated depletion quenchingTechniques
Computational DesignTarget processes
recombinationInput: Light
Implementation Constraints
The method uses light as the input modality and is described as a nonlinear optical strategy similar to STED. The supplied evidence identifies application to the Cph8 bidirectional optogenetic switch, but does not provide construct design details, wavelengths, pulse parameters, or delivery requirements.
The supplied evidence is limited to theoretical analysis, simulations, and general claims of implementation, with no detailed quantitative performance metrics provided here. Evidence for broad applicability is presented as an expectation or proposal rather than extensive cross-system experimental validation in the supplied record.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Source 2primary paper2018arXiv (Cornell University)
Source 3primary paper2018arXiv (Cornell University)
Ranked Claims
Increased selectivity of the photoactivation step via nonlinear optical techniques should translate into improved dynamic range for a broad variety of bidirectional switches.
Increased selectivity of the photoactivation step via nonlinear optical techniques should translate into an improved dynamic range for a broad variety of bidirectional switches.
Increased selectivity of the photoactivation step via nonlinear optical techniques should translate into improved dynamic range for a broad variety of bidirectional switches.
Increased selectivity of the photoactivation step via nonlinear optical techniques should translate into an improved dynamic range for a broad variety of bidirectional switches.
Increased selectivity of the photoactivation step via nonlinear optical techniques should translate into improved dynamic range for a broad variety of bidirectional switches.
Increased selectivity of the photoactivation step via nonlinear optical techniques should translate into an improved dynamic range for a broad variety of bidirectional switches.
Increased selectivity of the photoactivation step via nonlinear optical techniques should translate into improved dynamic range for a broad variety of bidirectional switches.
Increased selectivity of the photoactivation step via nonlinear optical techniques should translate into an improved dynamic range for a broad variety of bidirectional switches.
Increased selectivity of the photoactivation step via nonlinear optical techniques should translate into improved dynamic range for a broad variety of bidirectional switches.
Increased selectivity of the photoactivation step via nonlinear optical techniques should translate into an improved dynamic range for a broad variety of bidirectional switches.
Increased selectivity of the photoactivation step via nonlinear optical techniques should translate into improved dynamic range for a broad variety of bidirectional switches.
Increased selectivity of the photoactivation step via nonlinear optical techniques should translate into an improved dynamic range for a broad variety of bidirectional switches.
Increased selectivity of the photoactivation step via nonlinear optical techniques should translate into improved dynamic range for a broad variety of bidirectional switches.
Increased selectivity of the photoactivation step via nonlinear optical techniques should translate into an improved dynamic range for a broad variety of bidirectional switches.
Stimulated depletion quenching enhances photoactivation selectivity on one side of the switch and shifts the photoequilibrium beyond what is achievable with CW light.
Using stimulated depletion quenching (SDQ), which is a nonlinear optical strategy similar to STED, we demonstrate enhanced photoactivation selectivity on one side of the switch, thus shifting the photoequilibrium beyond what is achievable with CW light.
Stimulated depletion quenching enhances photoactivation selectivity on one side of the switch and shifts the photoequilibrium beyond what is achievable with CW light.
Using stimulated depletion quenching (SDQ), which is a nonlinear optical strategy similar to STED, we demonstrate enhanced photoactivation selectivity on one side of the switch, thus shifting the photoequilibrium beyond what is achievable with CW light.
Stimulated depletion quenching enhances photoactivation selectivity on one side of the switch and shifts the photoequilibrium beyond what is achievable with CW light.
Using stimulated depletion quenching (SDQ), which is a nonlinear optical strategy similar to STED, we demonstrate enhanced photoactivation selectivity on one side of the switch, thus shifting the photoequilibrium beyond what is achievable with CW light.
Stimulated depletion quenching enhances photoactivation selectivity on one side of the switch and shifts the photoequilibrium beyond what is achievable with CW light.
Using stimulated depletion quenching (SDQ), which is a nonlinear optical strategy similar to STED, we demonstrate enhanced photoactivation selectivity on one side of the switch, thus shifting the photoequilibrium beyond what is achievable with CW light.
Stimulated depletion quenching enhances photoactivation selectivity on one side of the switch and shifts the photoequilibrium beyond what is achievable with CW light.
Using stimulated depletion quenching (SDQ), which is a nonlinear optical strategy similar to STED, we demonstrate enhanced photoactivation selectivity on one side of the switch, thus shifting the photoequilibrium beyond what is achievable with CW light.
Stimulated depletion quenching enhances photoactivation selectivity on one side of the switch and shifts the photoequilibrium beyond what is achievable with CW light.
Using stimulated depletion quenching (SDQ), which is a nonlinear optical strategy similar to STED, we demonstrate enhanced photoactivation selectivity on one side of the switch, thus shifting the photoequilibrium beyond what is achievable with CW light.
Stimulated depletion quenching enhances photoactivation selectivity on one side of the switch and shifts the photoequilibrium beyond what is achievable with CW light.
Using stimulated depletion quenching (SDQ), which is a nonlinear optical strategy similar to STED, we demonstrate enhanced photoactivation selectivity on one side of the switch, thus shifting the photoequilibrium beyond what is achievable with CW light.
Stimulated Depletion Quenching is employed to enhance control of the Cph8 optogenetic switch.
SDQ is employed to enhance the control of Cph8, a photo-reversible phytochrome based optogenetic switch designed to control gene expression in E. Coli bacteria.
Stimulated Depletion Quenching is employed to enhance control of the Cph8 optogenetic switch.
SDQ is employed to enhance the control of Cph8, a photo-reversible phytochrome based optogenetic switch designed to control gene expression in E. Coli bacteria.
Stimulated Depletion Quenching is employed to enhance control of the Cph8 optogenetic switch.
SDQ is employed to enhance the control of Cph8, a photo-reversible phytochrome based optogenetic switch designed to control gene expression in E. Coli bacteria.
Stimulated Depletion Quenching is employed to enhance control of the Cph8 optogenetic switch.
SDQ is employed to enhance the control of Cph8, a photo-reversible phytochrome based optogenetic switch designed to control gene expression in E. Coli bacteria.
Stimulated Depletion Quenching is employed to enhance control of the Cph8 optogenetic switch.
SDQ is employed to enhance the control of Cph8, a photo-reversible phytochrome based optogenetic switch designed to control gene expression in E. Coli bacteria.
Stimulated Depletion Quenching is employed to enhance control of the Cph8 optogenetic switch.
SDQ is employed to enhance the control of Cph8, a photo-reversible phytochrome based optogenetic switch designed to control gene expression in E. Coli bacteria.
Stimulated Depletion Quenching is employed to enhance control of the Cph8 optogenetic switch.
SDQ is employed to enhance the control of Cph8, a photo-reversible phytochrome based optogenetic switch designed to control gene expression in E. Coli bacteria.
Stimulated Depletion Quenching was applied to the Cph8 bidirectional optogenetic switch.
This approach is applied to the phytochrome Cph8 bidirectional optogenetic switch
Stimulated Depletion Quenching was applied to the Cph8 bidirectional optogenetic switch.
This approach is applied to the phytochrome Cph8 bidirectional optogenetic switch
Stimulated Depletion Quenching was applied to the Cph8 bidirectional optogenetic switch.
This approach is applied to the phytochrome Cph8 bidirectional optogenetic switch
Stimulated Depletion Quenching was applied to the Cph8 bidirectional optogenetic switch.
This approach is applied to the phytochrome Cph8 bidirectional optogenetic switch
Stimulated Depletion Quenching was applied to the Cph8 bidirectional optogenetic switch.
This approach is applied to the phytochrome Cph8 bidirectional optogenetic switch
Stimulated Depletion Quenching was applied to the Cph8 bidirectional optogenetic switch.
This approach is applied to the phytochrome Cph8 bidirectional optogenetic switch
Stimulated Depletion Quenching was applied to the Cph8 bidirectional optogenetic switch.
This approach is applied to the phytochrome Cph8 bidirectional optogenetic switch
Linear photoswitching cannot fully convert Cph8 to the biologically inactive PFR state because spectral cross-talk drives reverse photoswitching back to the active PR state.
The Cph8 switch can not be fully converted to it's biologically inactive state ($P_{FR}$) by linear photos-witching, as spectral cross-talk causes a reverse photoswitching reaction to revert to it back to the active state ($P_{R}$).
Linear photoswitching cannot fully convert Cph8 to the biologically inactive PFR state because spectral cross-talk drives reverse photoswitching back to the active PR state.
The Cph8 switch can not be fully converted to it's biologically inactive state ($P_{FR}$) by linear photos-witching, as spectral cross-talk causes a reverse photoswitching reaction to revert to it back to the active state ($P_{R}$).
Linear photoswitching cannot fully convert Cph8 to the biologically inactive PFR state because spectral cross-talk drives reverse photoswitching back to the active PR state.
The Cph8 switch can not be fully converted to it's biologically inactive state ($P_{FR}$) by linear photos-witching, as spectral cross-talk causes a reverse photoswitching reaction to revert to it back to the active state ($P_{R}$).
Linear photoswitching cannot fully convert Cph8 to the biologically inactive PFR state because spectral cross-talk drives reverse photoswitching back to the active PR state.
The Cph8 switch can not be fully converted to it's biologically inactive state ($P_{FR}$) by linear photos-witching, as spectral cross-talk causes a reverse photoswitching reaction to revert to it back to the active state ($P_{R}$).
Linear photoswitching cannot fully convert Cph8 to the biologically inactive PFR state because spectral cross-talk drives reverse photoswitching back to the active PR state.
The Cph8 switch can not be fully converted to it's biologically inactive state ($P_{FR}$) by linear photos-witching, as spectral cross-talk causes a reverse photoswitching reaction to revert to it back to the active state ($P_{R}$).
Linear photoswitching cannot fully convert Cph8 to the biologically inactive PFR state because spectral cross-talk drives reverse photoswitching back to the active PR state.
The Cph8 switch can not be fully converted to it's biologically inactive state ($P_{FR}$) by linear photos-witching, as spectral cross-talk causes a reverse photoswitching reaction to revert to it back to the active state ($P_{R}$).
Linear photoswitching cannot fully convert Cph8 to the biologically inactive PFR state because spectral cross-talk drives reverse photoswitching back to the active PR state.
The Cph8 switch can not be fully converted to it's biologically inactive state ($P_{FR}$) by linear photos-witching, as spectral cross-talk causes a reverse photoswitching reaction to revert to it back to the active state ($P_{R}$).
Stimulated Depletion Quenching selectively halts the reverse photoswitching reaction of Cph8 while allowing the forward reaction to proceed.
SDQ selectively halts this reverse reaction while allowing the forward reaction to proceed.
Stimulated Depletion Quenching selectively halts the reverse photoswitching reaction of Cph8 while allowing the forward reaction to proceed.
SDQ selectively halts this reverse reaction while allowing the forward reaction to proceed.
Stimulated Depletion Quenching selectively halts the reverse photoswitching reaction of Cph8 while allowing the forward reaction to proceed.
SDQ selectively halts this reverse reaction while allowing the forward reaction to proceed.
Stimulated Depletion Quenching selectively halts the reverse photoswitching reaction of Cph8 while allowing the forward reaction to proceed.
SDQ selectively halts this reverse reaction while allowing the forward reaction to proceed.
Stimulated Depletion Quenching selectively halts the reverse photoswitching reaction of Cph8 while allowing the forward reaction to proceed.
SDQ selectively halts this reverse reaction while allowing the forward reaction to proceed.
Stimulated Depletion Quenching selectively halts the reverse photoswitching reaction of Cph8 while allowing the forward reaction to proceed.
SDQ selectively halts this reverse reaction while allowing the forward reaction to proceed.
Stimulated Depletion Quenching selectively halts the reverse photoswitching reaction of Cph8 while allowing the forward reaction to proceed.
SDQ selectively halts this reverse reaction while allowing the forward reaction to proceed.
Stimulated Depletion Quenching is used to overcome spectral cross-talk in optogenetic photoswitching.
Stimulated Depletion Quenching (SDQ), is used to overcome spectral cross-talk by exploiting the molecules' unique dynamic response to ultrashort laser pulses
Stimulated Depletion Quenching is used to overcome spectral cross-talk in optogenetic photoswitching.
Stimulated Depletion Quenching (SDQ), is used to overcome spectral cross-talk by exploiting the molecules' unique dynamic response to ultrashort laser pulses
Stimulated Depletion Quenching is used to overcome spectral cross-talk in optogenetic photoswitching.
Stimulated Depletion Quenching (SDQ), is used to overcome spectral cross-talk by exploiting the molecules' unique dynamic response to ultrashort laser pulses
Stimulated Depletion Quenching is used to overcome spectral cross-talk in optogenetic photoswitching.
Stimulated Depletion Quenching (SDQ), is used to overcome spectral cross-talk by exploiting the molecules' unique dynamic response to ultrashort laser pulses
Stimulated Depletion Quenching is used to overcome spectral cross-talk in optogenetic photoswitching.
Stimulated Depletion Quenching (SDQ), is used to overcome spectral cross-talk by exploiting the molecules' unique dynamic response to ultrashort laser pulses
Stimulated Depletion Quenching is used to overcome spectral cross-talk in optogenetic photoswitching.
Stimulated Depletion Quenching (SDQ), is used to overcome spectral cross-talk by exploiting the molecules' unique dynamic response to ultrashort laser pulses
Stimulated Depletion Quenching is used to overcome spectral cross-talk in optogenetic photoswitching.
Stimulated Depletion Quenching (SDQ), is used to overcome spectral cross-talk by exploiting the molecules' unique dynamic response to ultrashort laser pulses
The paper proposes Stimulated Depletion Quenching as a new control approach.
We propose and demonstrate through simulations a new control approach of Stimulated Depletion Quenching.
The paper proposes Stimulated Depletion Quenching as a new control approach.
We propose and demonstrate through simulations a new control approach of Stimulated Depletion Quenching.
The paper proposes Stimulated Depletion Quenching as a new control approach.
We propose and demonstrate through simulations a new control approach of Stimulated Depletion Quenching.
The paper proposes Stimulated Depletion Quenching as a new control approach.
We propose and demonstrate through simulations a new control approach of Stimulated Depletion Quenching.
The paper proposes Stimulated Depletion Quenching as a new control approach.
We propose and demonstrate through simulations a new control approach of Stimulated Depletion Quenching.
The paper proposes Stimulated Depletion Quenching as a new control approach.
We propose and demonstrate through simulations a new control approach of Stimulated Depletion Quenching.
The paper proposes Stimulated Depletion Quenching as a new control approach.
We propose and demonstrate through simulations a new control approach of Stimulated Depletion Quenching.
In simulations, applying Stimulated Depletion Quenching to the Cph8 bidirectional optogenetic switch showed significant improvement of dynamic range.
We propose and demonstrate through simulations a new control approach of Stimulated Depletion Quenching. This approach is applied to the phytochrome Cph8 bidirectional optogenetic switch, and the results show significant improvement of its dynamic range.
In simulations, applying Stimulated Depletion Quenching to the Cph8 bidirectional optogenetic switch showed significant improvement of dynamic range.
We propose and demonstrate through simulations a new control approach of Stimulated Depletion Quenching. This approach is applied to the phytochrome Cph8 bidirectional optogenetic switch, and the results show significant improvement of its dynamic range.
In simulations, applying Stimulated Depletion Quenching to the Cph8 bidirectional optogenetic switch showed significant improvement of dynamic range.
We propose and demonstrate through simulations a new control approach of Stimulated Depletion Quenching. This approach is applied to the phytochrome Cph8 bidirectional optogenetic switch, and the results show significant improvement of its dynamic range.
In simulations, applying Stimulated Depletion Quenching to the Cph8 bidirectional optogenetic switch showed significant improvement of dynamic range.
We propose and demonstrate through simulations a new control approach of Stimulated Depletion Quenching. This approach is applied to the phytochrome Cph8 bidirectional optogenetic switch, and the results show significant improvement of its dynamic range.
In simulations, applying Stimulated Depletion Quenching to the Cph8 bidirectional optogenetic switch showed significant improvement of dynamic range.
We propose and demonstrate through simulations a new control approach of Stimulated Depletion Quenching. This approach is applied to the phytochrome Cph8 bidirectional optogenetic switch, and the results show significant improvement of its dynamic range.
In simulations, applying Stimulated Depletion Quenching to the Cph8 bidirectional optogenetic switch showed significant improvement of dynamic range.
We propose and demonstrate through simulations a new control approach of Stimulated Depletion Quenching. This approach is applied to the phytochrome Cph8 bidirectional optogenetic switch, and the results show significant improvement of its dynamic range.
In simulations, applying Stimulated Depletion Quenching to the Cph8 bidirectional optogenetic switch showed significant improvement of dynamic range.
We propose and demonstrate through simulations a new control approach of Stimulated Depletion Quenching. This approach is applied to the phytochrome Cph8 bidirectional optogenetic switch, and the results show significant improvement of its dynamic range.
Multiplexed control of several optogenetic components is challenged by significant spectral cross talk.
The major challenge is the multiplexed control of several optogenetic components in the presence of significant spectral cross talk.
Multiplexed control of several optogenetic components is challenged by significant spectral cross talk.
The major challenge is the multiplexed control of several optogenetic components in the presence of significant spectral cross talk.
Multiplexed control of several optogenetic components is challenged by significant spectral cross talk.
The major challenge is the multiplexed control of several optogenetic components in the presence of significant spectral cross talk.
Multiplexed control of several optogenetic components is challenged by significant spectral cross talk.
The major challenge is the multiplexed control of several optogenetic components in the presence of significant spectral cross talk.
Multiplexed control of several optogenetic components is challenged by significant spectral cross talk.
The major challenge is the multiplexed control of several optogenetic components in the presence of significant spectral cross talk.
Multiplexed control of several optogenetic components is challenged by significant spectral cross talk.
The major challenge is the multiplexed control of several optogenetic components in the presence of significant spectral cross talk.
Multiplexed control of several optogenetic components is challenged by significant spectral cross talk.
The major challenge is the multiplexed control of several optogenetic components in the presence of significant spectral cross talk.
Spectral cross-talk hampers optogenetic switches by limiting independent control and restricting dynamic range.
the technique is hampered by spectral cross-talk: the broad absorption spectra of compatible biochemical chromophores limits the number of switches that can be independently controlled and restricts the dynamic range of each switch
Spectral cross-talk hampers optogenetic switches by limiting independent control and restricting dynamic range.
the technique is hampered by spectral cross-talk: the broad absorption spectra of compatible biochemical chromophores limits the number of switches that can be independently controlled and restricts the dynamic range of each switch
Spectral cross-talk hampers optogenetic switches by limiting independent control and restricting dynamic range.
the technique is hampered by spectral cross-talk: the broad absorption spectra of compatible biochemical chromophores limits the number of switches that can be independently controlled and restricts the dynamic range of each switch
Spectral cross-talk hampers optogenetic switches by limiting independent control and restricting dynamic range.
the technique is hampered by spectral cross-talk: the broad absorption spectra of compatible biochemical chromophores limits the number of switches that can be independently controlled and restricts the dynamic range of each switch
Spectral cross-talk hampers optogenetic switches by limiting independent control and restricting dynamic range.
the technique is hampered by spectral cross-talk: the broad absorption spectra of compatible biochemical chromophores limits the number of switches that can be independently controlled and restricts the dynamic range of each switch
Spectral cross-talk hampers optogenetic switches by limiting independent control and restricting dynamic range.
the technique is hampered by spectral cross-talk: the broad absorption spectra of compatible biochemical chromophores limits the number of switches that can be independently controlled and restricts the dynamic range of each switch
Spectral cross-talk hampers optogenetic switches by limiting independent control and restricting dynamic range.
the technique is hampered by spectral cross-talk: the broad absorption spectra of compatible biochemical chromophores limits the number of switches that can be independently controlled and restricts the dynamic range of each switch
Approval Evidence
3 sources9 linked approval claimsfirst-pass slug stimulated-depletion-quenching
Using stimulated depletion quenching (SDQ), which is a nonlinear optical strategy similar to STED, we demonstrate enhanced photoactivation selectivity
Source:
In the present work we develop and implement a non-linear optical photoswitching capability, Stimulated Depletion Quenching (SDQ)
Source:
We propose and demonstrate through simulations a new control approach of Stimulated Depletion Quenching.
Source:
application scopesupports
Increased selectivity of the photoactivation step via nonlinear optical techniques should translate into improved dynamic range for a broad variety of bidirectional switches.
Increased selectivity of the photoactivation step via nonlinear optical techniques should translate into an improved dynamic range for a broad variety of bidirectional switches.
Source:
performance improvementsupports
Stimulated depletion quenching enhances photoactivation selectivity on one side of the switch and shifts the photoequilibrium beyond what is achievable with CW light.
Using stimulated depletion quenching (SDQ), which is a nonlinear optical strategy similar to STED, we demonstrate enhanced photoactivation selectivity on one side of the switch, thus shifting the photoequilibrium beyond what is achievable with CW light.
Source:
applicationsupports
Stimulated Depletion Quenching is employed to enhance control of the Cph8 optogenetic switch.
SDQ is employed to enhance the control of Cph8, a photo-reversible phytochrome based optogenetic switch designed to control gene expression in E. Coli bacteria.
Source:
applicationsupports
Stimulated Depletion Quenching was applied to the Cph8 bidirectional optogenetic switch.
This approach is applied to the phytochrome Cph8 bidirectional optogenetic switch
Source:
mechanismsupports
Stimulated Depletion Quenching selectively halts the reverse photoswitching reaction of Cph8 while allowing the forward reaction to proceed.
SDQ selectively halts this reverse reaction while allowing the forward reaction to proceed.
Source:
method capabilitysupports
Stimulated Depletion Quenching is used to overcome spectral cross-talk in optogenetic photoswitching.
Stimulated Depletion Quenching (SDQ), is used to overcome spectral cross-talk by exploiting the molecules' unique dynamic response to ultrashort laser pulses
Source:
method proposalsupports
The paper proposes Stimulated Depletion Quenching as a new control approach.
We propose and demonstrate through simulations a new control approach of Stimulated Depletion Quenching.
Source:
performance improvementsupports
In simulations, applying Stimulated Depletion Quenching to the Cph8 bidirectional optogenetic switch showed significant improvement of dynamic range.
We propose and demonstrate through simulations a new control approach of Stimulated Depletion Quenching. This approach is applied to the phytochrome Cph8 bidirectional optogenetic switch, and the results show significant improvement of its dynamic range.
Source:
problem statementsupports
Multiplexed control of several optogenetic components is challenged by significant spectral cross talk.
The major challenge is the multiplexed control of several optogenetic components in the presence of significant spectral cross talk.
Source:
Comparisons
Source-backed strengths
The cited literature reports that SDQ enhances photoactivation selectivity on one side of the switch and shifts the photoequilibrium beyond the limit achievable with continuous-wave light. It was developed as a nonlinear optical photoswitching capability and analyzed or demonstrated in the context of the Cph8 bidirectional optogenetic switch.
Source:
Using stimulated depletion quenching (SDQ), which is a nonlinear optical strategy similar to STED, we demonstrate enhanced photoactivation selectivity on one side of the switch, thus shifting the photoequilibrium beyond what is achievable with CW light.
Source:
We propose and demonstrate through simulations a new control approach of Stimulated Depletion Quenching. This approach is applied to the phytochrome Cph8 bidirectional optogenetic switch, and the results show significant improvement of its dynamic range.
Ranked Citations
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