Source 1review2018Biological Chemistry
Toolkit/switchable inteins
switchable inteins
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Switchable inteins are conditional protein-splicing systems in which intein activity is regulated post-translationally to control the state of pre-existing proteins. The cited review specifically discusses strategies for controlling intein activity, with emphasis on approaches intended for use in living cells.
Usefulness & Problems
Why this is useful
These systems are useful because post-translational control of pre-existing proteins can provide faster responses than regulation at the level of gene expression. The available evidence supports their value as a framework for conditional control of protein function in cellular contexts, but does not provide specific application benchmarks.
Source:
Conditional splicing of internal protein domains, termed inteins, is an attractive methodology for this purpose.
Problem solved
Switchable inteins address the problem of how to regulate protein state after translation rather than by controlling synthesis of the encoded protein. The cited evidence supports this as a strategy for achieving faster functional responses, particularly for live-cell-compatible protein control.
Source:
Conditional splicing of internal protein domains, termed inteins, is an attractive methodology for this purpose.
Problem links
Need inducible protein relocalization or recruitment
DerivedSwitchable inteins are conditional protein-splicing systems in which intein activity is controlled post-translationally to regulate the state of pre-existing proteins. The cited review focuses on strategies for controlling intein activity, particularly approaches intended to be compatible with applications in living cells.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
conditional protein splicingconditional protein splicingpost-translational controlpost-translational controlTechniques
No technique tags yet.
Target processes
localizationImplementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: multi component delivery burdenoperating role: actuatoroperating role: regulatorswitch architecture: multi component
The available evidence only states that the reviewed control strategies focus on compatibility with applications in living cells. No specific construct architectures, cofactors, delivery methods, expression systems, or trigger modalities are described in the supplied material.
The supplied evidence is limited to high-level review statements and does not identify specific intein systems, triggers, host organisms, or quantitative performance metrics. It also does not document independent experimental validation, dynamic range, background splicing, or localization-specific case studies.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Post-translational modification of pre-existing proteins can provide faster responses than controlling proteins by regulating expression of their encoding genes.
Proteins can be controlled in several ways, for instance by regulating the expression of their encoding genes with small molecules or light. However, post-translationally modifying pre-existing proteins to regulate their function or localization leads to faster responses.
Post-translational modification of pre-existing proteins can provide faster responses than controlling proteins by regulating expression of their encoding genes.
Proteins can be controlled in several ways, for instance by regulating the expression of their encoding genes with small molecules or light. However, post-translationally modifying pre-existing proteins to regulate their function or localization leads to faster responses.
Post-translational modification of pre-existing proteins can provide faster responses than controlling proteins by regulating expression of their encoding genes.
Proteins can be controlled in several ways, for instance by regulating the expression of their encoding genes with small molecules or light. However, post-translationally modifying pre-existing proteins to regulate their function or localization leads to faster responses.
Post-translational modification of pre-existing proteins can provide faster responses than controlling proteins by regulating expression of their encoding genes.
Proteins can be controlled in several ways, for instance by regulating the expression of their encoding genes with small molecules or light. However, post-translationally modifying pre-existing proteins to regulate their function or localization leads to faster responses.
Post-translational modification of pre-existing proteins can provide faster responses than controlling proteins by regulating expression of their encoding genes.
Proteins can be controlled in several ways, for instance by regulating the expression of their encoding genes with small molecules or light. However, post-translationally modifying pre-existing proteins to regulate their function or localization leads to faster responses.
Post-translational modification of pre-existing proteins can provide faster responses than controlling proteins by regulating expression of their encoding genes.
Proteins can be controlled in several ways, for instance by regulating the expression of their encoding genes with small molecules or light. However, post-translationally modifying pre-existing proteins to regulate their function or localization leads to faster responses.
Post-translational modification of pre-existing proteins can provide faster responses than controlling proteins by regulating expression of their encoding genes.
Proteins can be controlled in several ways, for instance by regulating the expression of their encoding genes with small molecules or light. However, post-translationally modifying pre-existing proteins to regulate their function or localization leads to faster responses.
Post-translational modification of pre-existing proteins can provide faster responses than controlling proteins by regulating expression of their encoding genes.
Proteins can be controlled in several ways, for instance by regulating the expression of their encoding genes with small molecules or light. However, post-translationally modifying pre-existing proteins to regulate their function or localization leads to faster responses.
Post-translational modification of pre-existing proteins can provide faster responses than controlling proteins by regulating expression of their encoding genes.
Proteins can be controlled in several ways, for instance by regulating the expression of their encoding genes with small molecules or light. However, post-translationally modifying pre-existing proteins to regulate their function or localization leads to faster responses.
Post-translational modification of pre-existing proteins can provide faster responses than controlling proteins by regulating expression of their encoding genes.
Proteins can be controlled in several ways, for instance by regulating the expression of their encoding genes with small molecules or light. However, post-translationally modifying pre-existing proteins to regulate their function or localization leads to faster responses.
Post-translational modification of pre-existing proteins can provide faster responses than controlling proteins by regulating expression of their encoding genes.
Proteins can be controlled in several ways, for instance by regulating the expression of their encoding genes with small molecules or light. However, post-translationally modifying pre-existing proteins to regulate their function or localization leads to faster responses.
Post-translational modification of pre-existing proteins can provide faster responses than controlling proteins by regulating expression of their encoding genes.
Proteins can be controlled in several ways, for instance by regulating the expression of their encoding genes with small molecules or light. However, post-translationally modifying pre-existing proteins to regulate their function or localization leads to faster responses.
Post-translational modification of pre-existing proteins can provide faster responses than controlling proteins by regulating expression of their encoding genes.
Proteins can be controlled in several ways, for instance by regulating the expression of their encoding genes with small molecules or light. However, post-translationally modifying pre-existing proteins to regulate their function or localization leads to faster responses.
Post-translational modification of pre-existing proteins can provide faster responses than controlling proteins by regulating expression of their encoding genes.
Proteins can be controlled in several ways, for instance by regulating the expression of their encoding genes with small molecules or light. However, post-translationally modifying pre-existing proteins to regulate their function or localization leads to faster responses.
Post-translational modification of pre-existing proteins can provide faster responses than controlling proteins by regulating expression of their encoding genes.
Proteins can be controlled in several ways, for instance by regulating the expression of their encoding genes with small molecules or light. However, post-translationally modifying pre-existing proteins to regulate their function or localization leads to faster responses.
Post-translational modification of pre-existing proteins can provide faster responses than controlling proteins by regulating expression of their encoding genes.
Proteins can be controlled in several ways, for instance by regulating the expression of their encoding genes with small molecules or light. However, post-translationally modifying pre-existing proteins to regulate their function or localization leads to faster responses.
Post-translational modification of pre-existing proteins can provide faster responses than controlling proteins by regulating expression of their encoding genes.
Proteins can be controlled in several ways, for instance by regulating the expression of their encoding genes with small molecules or light. However, post-translationally modifying pre-existing proteins to regulate their function or localization leads to faster responses.
Post-translational modification of pre-existing proteins can provide faster responses than controlling proteins by regulating expression of their encoding genes.
Proteins can be controlled in several ways, for instance by regulating the expression of their encoding genes with small molecules or light. However, post-translationally modifying pre-existing proteins to regulate their function or localization leads to faster responses.
Post-translational modification of pre-existing proteins can provide faster responses than controlling proteins by regulating expression of their encoding genes.
Proteins can be controlled in several ways, for instance by regulating the expression of their encoding genes with small molecules or light. However, post-translationally modifying pre-existing proteins to regulate their function or localization leads to faster responses.
Post-translational modification of pre-existing proteins can provide faster responses than controlling proteins by regulating expression of their encoding genes.
Proteins can be controlled in several ways, for instance by regulating the expression of their encoding genes with small molecules or light. However, post-translationally modifying pre-existing proteins to regulate their function or localization leads to faster responses.
The review discusses methods to control intein activity with a focus on approaches compatible with applications in living cells.
Here we discuss methods to control intein activity with a focus on those compatible with applications in living cells.
The review discusses methods to control intein activity with a focus on approaches compatible with applications in living cells.
Here we discuss methods to control intein activity with a focus on those compatible with applications in living cells.
The review discusses methods to control intein activity with a focus on approaches compatible with applications in living cells.
Here we discuss methods to control intein activity with a focus on those compatible with applications in living cells.
The review discusses methods to control intein activity with a focus on approaches compatible with applications in living cells.
Here we discuss methods to control intein activity with a focus on those compatible with applications in living cells.
The review discusses methods to control intein activity with a focus on approaches compatible with applications in living cells.
Here we discuss methods to control intein activity with a focus on those compatible with applications in living cells.
The review discusses methods to control intein activity with a focus on approaches compatible with applications in living cells.
Here we discuss methods to control intein activity with a focus on those compatible with applications in living cells.
The review discusses methods to control intein activity with a focus on approaches compatible with applications in living cells.
Here we discuss methods to control intein activity with a focus on those compatible with applications in living cells.
The review discusses methods to control intein activity with a focus on approaches compatible with applications in living cells.
Here we discuss methods to control intein activity with a focus on those compatible with applications in living cells.
The review discusses methods to control intein activity with a focus on approaches compatible with applications in living cells.
Here we discuss methods to control intein activity with a focus on those compatible with applications in living cells.
The review discusses methods to control intein activity with a focus on approaches compatible with applications in living cells.
Here we discuss methods to control intein activity with a focus on those compatible with applications in living cells.
The review discusses methods to control intein activity with a focus on approaches compatible with applications in living cells.
Here we discuss methods to control intein activity with a focus on those compatible with applications in living cells.
The review discusses methods to control intein activity with a focus on approaches compatible with applications in living cells.
Here we discuss methods to control intein activity with a focus on those compatible with applications in living cells.
The review discusses methods to control intein activity with a focus on approaches compatible with applications in living cells.
Here we discuss methods to control intein activity with a focus on those compatible with applications in living cells.
The review discusses methods to control intein activity with a focus on approaches compatible with applications in living cells.
Here we discuss methods to control intein activity with a focus on those compatible with applications in living cells.
The review discusses methods to control intein activity with a focus on approaches compatible with applications in living cells.
Here we discuss methods to control intein activity with a focus on those compatible with applications in living cells.
The review discusses methods to control intein activity with a focus on approaches compatible with applications in living cells.
Here we discuss methods to control intein activity with a focus on those compatible with applications in living cells.
The review discusses methods to control intein activity with a focus on approaches compatible with applications in living cells.
Here we discuss methods to control intein activity with a focus on those compatible with applications in living cells.
The review discusses methods to control intein activity with a focus on approaches compatible with applications in living cells.
Here we discuss methods to control intein activity with a focus on those compatible with applications in living cells.
The review discusses methods to control intein activity with a focus on approaches compatible with applications in living cells.
Here we discuss methods to control intein activity with a focus on those compatible with applications in living cells.
The review discusses methods to control intein activity with a focus on approaches compatible with applications in living cells.
Here we discuss methods to control intein activity with a focus on those compatible with applications in living cells.
The review discusses methods to control intein activity with a focus on approaches compatible with applications in living cells.
Here we discuss methods to control intein activity with a focus on those compatible with applications in living cells.
The review discusses methods to control intein activity with a focus on approaches compatible with applications in living cells.
Here we discuss methods to control intein activity with a focus on those compatible with applications in living cells.
The review discusses methods to control intein activity with a focus on approaches compatible with applications in living cells.
Here we discuss methods to control intein activity with a focus on those compatible with applications in living cells.
The review discusses methods to control intein activity with a focus on approaches compatible with applications in living cells.
Here we discuss methods to control intein activity with a focus on those compatible with applications in living cells.
The review discusses methods to control intein activity with a focus on approaches compatible with applications in living cells.
Here we discuss methods to control intein activity with a focus on those compatible with applications in living cells.
The review discusses methods to control intein activity with a focus on approaches compatible with applications in living cells.
Here we discuss methods to control intein activity with a focus on those compatible with applications in living cells.
The review discusses methods to control intein activity with a focus on approaches compatible with applications in living cells.
Here we discuss methods to control intein activity with a focus on those compatible with applications in living cells.
Conditional splicing mediated by inteins is presented as an attractive method for controlling protein function or localization.
Conditional splicing of internal protein domains, termed inteins, is an attractive methodology for this purpose.
Conditional splicing mediated by inteins is presented as an attractive method for controlling protein function or localization.
Conditional splicing of internal protein domains, termed inteins, is an attractive methodology for this purpose.
Conditional splicing mediated by inteins is presented as an attractive method for controlling protein function or localization.
Conditional splicing of internal protein domains, termed inteins, is an attractive methodology for this purpose.
Conditional splicing mediated by inteins is presented as an attractive method for controlling protein function or localization.
Conditional splicing of internal protein domains, termed inteins, is an attractive methodology for this purpose.
Conditional splicing mediated by inteins is presented as an attractive method for controlling protein function or localization.
Conditional splicing of internal protein domains, termed inteins, is an attractive methodology for this purpose.
Conditional splicing mediated by inteins is presented as an attractive method for controlling protein function or localization.
Conditional splicing of internal protein domains, termed inteins, is an attractive methodology for this purpose.
Conditional splicing mediated by inteins is presented as an attractive method for controlling protein function or localization.
Conditional splicing of internal protein domains, termed inteins, is an attractive methodology for this purpose.
Conditional splicing mediated by inteins is presented as an attractive method for controlling protein function or localization.
Conditional splicing of internal protein domains, termed inteins, is an attractive methodology for this purpose.
Conditional splicing mediated by inteins is presented as an attractive method for controlling protein function or localization.
Conditional splicing of internal protein domains, termed inteins, is an attractive methodology for this purpose.
Conditional splicing mediated by inteins is presented as an attractive method for controlling protein function or localization.
Conditional splicing of internal protein domains, termed inteins, is an attractive methodology for this purpose.
Conditional splicing mediated by inteins is presented as an attractive method for controlling protein function or localization.
Conditional splicing of internal protein domains, termed inteins, is an attractive methodology for this purpose.
Conditional splicing mediated by inteins is presented as an attractive method for controlling protein function or localization.
Conditional splicing of internal protein domains, termed inteins, is an attractive methodology for this purpose.
Conditional splicing mediated by inteins is presented as an attractive method for controlling protein function or localization.
Conditional splicing of internal protein domains, termed inteins, is an attractive methodology for this purpose.
Conditional splicing mediated by inteins is presented as an attractive method for controlling protein function or localization.
Conditional splicing of internal protein domains, termed inteins, is an attractive methodology for this purpose.
Conditional splicing mediated by inteins is presented as an attractive method for controlling protein function or localization.
Conditional splicing of internal protein domains, termed inteins, is an attractive methodology for this purpose.
Conditional splicing mediated by inteins is presented as an attractive method for controlling protein function or localization.
Conditional splicing of internal protein domains, termed inteins, is an attractive methodology for this purpose.
Conditional splicing mediated by inteins is presented as an attractive method for controlling protein function or localization.
Conditional splicing of internal protein domains, termed inteins, is an attractive methodology for this purpose.
Conditional splicing mediated by inteins is presented as an attractive method for controlling protein function or localization.
Conditional splicing of internal protein domains, termed inteins, is an attractive methodology for this purpose.
Conditional splicing mediated by inteins is presented as an attractive method for controlling protein function or localization.
Conditional splicing of internal protein domains, termed inteins, is an attractive methodology for this purpose.
Conditional splicing mediated by inteins is presented as an attractive method for controlling protein function or localization.
Conditional splicing of internal protein domains, termed inteins, is an attractive methodology for this purpose.
Conditional splicing mediated by inteins is presented as an attractive method for controlling protein function or localization.
Conditional splicing of internal protein domains, termed inteins, is an attractive methodology for this purpose.
Conditional splicing mediated by inteins is presented as an attractive method for controlling protein function or localization.
Conditional splicing of internal protein domains, termed inteins, is an attractive methodology for this purpose.
Conditional splicing mediated by inteins is presented as an attractive method for controlling protein function or localization.
Conditional splicing of internal protein domains, termed inteins, is an attractive methodology for this purpose.
Conditional splicing mediated by inteins is presented as an attractive method for controlling protein function or localization.
Conditional splicing of internal protein domains, termed inteins, is an attractive methodology for this purpose.
Conditional splicing mediated by inteins is presented as an attractive method for controlling protein function or localization.
Conditional splicing of internal protein domains, termed inteins, is an attractive methodology for this purpose.
Conditional splicing mediated by inteins is presented as an attractive method for controlling protein function or localization.
Conditional splicing of internal protein domains, termed inteins, is an attractive methodology for this purpose.
Conditional splicing mediated by inteins is presented as an attractive method for controlling protein function or localization.
Conditional splicing of internal protein domains, termed inteins, is an attractive methodology for this purpose.
Approval Evidence
1 source2 linked approval claimsfirst-pass slug switchable-inteins
Switchable inteins for conditional protein splicing
Source:
review scope statementneutral
The review discusses methods to control intein activity with a focus on approaches compatible with applications in living cells.
Here we discuss methods to control intein activity with a focus on those compatible with applications in living cells.
Source:
use casesupports
Conditional splicing mediated by inteins is presented as an attractive method for controlling protein function or localization.
Conditional splicing of internal protein domains, termed inteins, is an attractive methodology for this purpose.
Source:
Comparisons
Source-backed strengths
A key stated advantage is the potential for faster response kinetics relative to gene-expression-based control, because modulation occurs on pre-existing proteins. The review also indicates that methods have been developed with compatibility for applications in living cells as an explicit design consideration.
Source:
Proteins can be controlled in several ways, for instance by regulating the expression of their encoding genes with small molecules or light. However, post-translationally modifying pre-existing proteins to regulate their function or localization leads to faster responses.
Compared with CLASP
switchable inteins and CLASP address a similar problem space because they share localization.
Shared frame: same top-level item type; shared target processes: localization
Strengths here: looks easier to implement in practice.
Compared with iLID/SspB
switchable inteins and iLID/SspB address a similar problem space because they share localization.
Shared frame: same top-level item type; shared target processes: localization
Relative tradeoffs: appears more independently replicated.
Compared with synthetic condensates
switchable inteins and synthetic condensates address a similar problem space because they share localization.
Shared frame: same top-level item type; shared target processes: localization
Ranked Citations
- 1.