Source 1primary paper2020
Toolkit/synPCB
synPCB
Also known as: improved synPCB, PCB synthesis system
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
synPCB is a genetically encoded phycocyanobilin synthesis system that supplies the chromophore required for PhyB-PIF phytochrome-based optogenetics. The improved synPCB version increased PCB production by approximately 4-fold and was incorporated with PhyB-PIF into doxycycline-inducible lentiviral and transposon vectors to support light-inducible dimerization system expression or function.
Usefulness & Problems
Why this is useful
synPCB is useful because it enables intracellular production of phycocyanobilin, a required cofactor for PhyB-PIF optogenetic systems. This reduces dependence on exogenous chromophore supply and supports deployment of PhyB-PIF light-inducible dimerization in inducible vector formats.
Source:
resulting in an approximately 4-fold increase in PCB synthesis compared with the previous version
Problem solved
synPCB addresses the need to provide PCB for PhyB-based optogenetics, since the required chromophore is not generally available in typical non-plant, non-cyanobacterial contexts. The improved design specifically addresses insufficient PCB output by increasing synthesis relative to the previous synPCB version.
Problem links
Need conditional recombination or state switching
DerivedsynPCB is a genetically encoded phycocyanobilin (PCB) synthesis system that supplies the chromophore required for PhyB-PIF phytochrome-based optogenetics. The improved synPCB design increased PCB production by approximately 4-fold and was incorporated with PhyB-PIF into doxycycline-inducible lentiviral and transposon vectors to support light-inducible dimerization system expression or function.
Need precise spatiotemporal control with light input
DerivedsynPCB is a genetically encoded phycocyanobilin (PCB) synthesis system that supplies the chromophore required for PhyB-PIF phytochrome-based optogenetics. The improved synPCB design increased PCB production by approximately 4-fold and was incorporated with PhyB-PIF into doxycycline-inducible lentiviral and transposon vectors to support light-inducible dimerization system expression or function.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Target processes
recombinationInput: Light
Implementation Constraints
cofactor dependency: requires exogenous cofactorencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: multi component delivery burdenimplementation constraint: payload burdenimplementation constraint: spectral hardware requirementoperating role: regulatorswitch architecture: multi componentswitch architecture: recruitment
The system is described as using four PCB synthesis genes, including thermophilic cyanobacterial counterparts, a tFnr-Fd chimera, and P2A-linked polycistronic expression. It was incorporated with PhyB-PIF into doxycycline-inducible lentiviral and transposon vectors to enable inducible PCB synthesis and support PhyB-PIF system expression or function.
The evidence indicates that synPCB supplies chromophore support for PhyB-PIF rather than acting as the light-responsive switch itself. The provided evidence does not report standalone control of a biological process by synPCB, nor does it provide broader validation across multiple cell types, organisms, or application settings.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Drug inducible lentiviral and transposon vectors carrying PhyB-PIF and synPCB enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Drug inducible lentiviral and transposon vectors carrying PhyB-PIF and synPCB enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Drug inducible lentiviral and transposon vectors carrying PhyB-PIF and synPCB enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Incorporation of PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Concatenating the PCB synthesis genes with P2A peptide cDNAs for polycistronic expression resulted in an approximately 4-fold increase in PCB synthesis compared with the previous version.
these genes were concatenated with P2A peptide cDNAs for polycistronic expression, resulting in an approximately 4-fold increase in PCB synthesis compared with the previous version
PCB synthesis increase 4 fold
The improved synPCB design increased PCB synthesis by approximately 4-fold compared with the previous version.
resulting in an approximately 4-fold increase in PCB synthesis compared with the previous version
PCB synthesis increase versus previous version approximately 4-fold
The improved synPCB design increased PCB synthesis by approximately 4-fold compared with the previous version.
resulting in an approximately 4-fold increase in PCB synthesis compared with the previous version
PCB synthesis increase versus previous version approximately 4-fold
The improved synPCB design increased PCB synthesis by approximately 4-fold compared with the previous version.
resulting in an approximately 4-fold increase in PCB synthesis compared with the previous version
PCB synthesis increase versus previous version approximately 4-fold
Approval Evidence
1 source4 linked approval claimsfirst-pass slug synpcb
Here, we report that we further improved our previously developed PCB synthesis system (synPCB)
Source:
we further improved our previously developed PCB synthesis system (synPCB)
Source:
inducible controlsupports
Drug inducible lentiviral and transposon vectors carrying PhyB-PIF and synPCB enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Source:
inducible expression capabilitysupports
Incorporation of PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Source:
performance improvementsupports
Concatenating the PCB synthesis genes with P2A peptide cDNAs for polycistronic expression resulted in an approximately 4-fold increase in PCB synthesis compared with the previous version.
these genes were concatenated with P2A peptide cDNAs for polycistronic expression, resulting in an approximately 4-fold increase in PCB synthesis compared with the previous version
Source:
tool improvementsupports
The improved synPCB design increased PCB synthesis by approximately 4-fold compared with the previous version.
resulting in an approximately 4-fold increase in PCB synthesis compared with the previous version
Source:
Comparisons
Source-backed strengths
The improved synPCB design produced an approximately 4-fold increase in PCB synthesis compared with the previous version. Concatenation of the PCB synthesis genes with P2A peptide cDNAs enabled polycistronic expression, and the system was further implemented in doxycycline-inducible lentiviral and transposon vectors together with PhyB-PIF.
Source:
these genes were concatenated with P2A peptide cDNAs for polycistronic expression, resulting in an approximately 4-fold increase in PCB synthesis compared with the previous version
Compared with AQTrip EL222 variant
synPCB and AQTrip EL222 variant address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination; shared mechanisms: heterodimerization; same primary input modality: light
Relative tradeoffs: may avoid an exogenous cofactor requirement.
synPCB and CRY2-talin/CIBN-CAAX optogenetic plasma membrane recruitment system address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination; shared mechanisms: heterodimerization; same primary input modality: light
Relative tradeoffs: may avoid an exogenous cofactor requirement.
Compared with PA-Cre 3.0
synPCB and PA-Cre 3.0 address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination; shared mechanisms: heterodimerization; same primary input modality: light
Relative tradeoffs: may avoid an exogenous cofactor requirement.
Ranked Citations
- 1.