Source 1review2023ChemBioChem
Toolkit/synthetic multienzyme complexes
synthetic multienzyme complexes
Also known as: synthetic multienzyme assemblies
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Synthetic multienzyme complexes are engineered multienzyme assemblies, spanning static enzyme nanostructures to dynamic enzyme coacervates, that arrange sequential biosynthetic enzymes in metabolon-like configurations. Some complexes assembled inside microbial cells can be isolated as independent catalytic entities and used for ex vivo biosynthesis.
Usefulness & Problems
Why this is useful
These assemblies are useful for organizing sequential biosynthetic enzymes so that active sites are brought into proximity during pathway operation. This arrangement is reported to promote intermediate transfer, reduce intermediate leakage, and streamline metabolic flux toward desired products.
Source:
Enzyme complexes constructed inside microbial cells can be separated as independent entities and catalyze biosynthetic reactions ex vivo; such a feature gains these complexes another name, "synthetic organelles" - new subcellular entities with independent structures and functions.
Source:
The formation of multienzyme complexes, or metabolons, brings the enzyme active sites into proximity to promote intermediate transfer, decrease intermediate leakage, and streamline the metabolic flux towards the desired products.
Problem solved
Synthetic multienzyme complexes address the problem of inefficient transfer of pathway intermediates between sequential enzymes in biosynthetic systems. The cited rationale is that metabolon-like assembly improves handling of intermediates and supports more directed flux to target products.
Problem links
Need conditional recombination or state switching
DerivedSynthetic multienzyme complexes are engineered multienzyme assemblies, spanning static enzyme nanostructures to dynamic enzyme coacervates, that arrange sequential biosynthetic enzymes in metabolon-like configurations. Some complexes assembled inside microbial cells can be isolated as independent catalytic entities and used for ex vivo biosynthesis.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Techniques
No technique tags yet.
Target processes
recombinationInput: Chemical
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: regulator
The evidence indicates that these complexes can be constructed inside microbial cells and, in some cases, isolated afterward as independent catalytic entities. Beyond this intracellular construction and isolation concept, the supplied material does not specify cofactors, expression systems, construct architectures, or purification requirements.
The supplied evidence does not provide quantitative performance data, specific pathway examples, or comparative benchmarks against unassembled enzymes. It also does not define the molecular scaffolding strategies, host range, or the generality of ex vivo isolation across different assemblies.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Some enzyme complexes built inside microbial cells can be isolated as independent entities and catalyze biosynthetic reactions ex vivo, motivating the term synthetic organelles.
Enzyme complexes constructed inside microbial cells can be separated as independent entities and catalyze biosynthetic reactions ex vivo; such a feature gains these complexes another name, "synthetic organelles" - new subcellular entities with independent structures and functions.
Some enzyme complexes built inside microbial cells can be isolated as independent entities and catalyze biosynthetic reactions ex vivo, motivating the term synthetic organelles.
Enzyme complexes constructed inside microbial cells can be separated as independent entities and catalyze biosynthetic reactions ex vivo; such a feature gains these complexes another name, "synthetic organelles" - new subcellular entities with independent structures and functions.
Some enzyme complexes built inside microbial cells can be isolated as independent entities and catalyze biosynthetic reactions ex vivo, motivating the term synthetic organelles.
Enzyme complexes constructed inside microbial cells can be separated as independent entities and catalyze biosynthetic reactions ex vivo; such a feature gains these complexes another name, "synthetic organelles" - new subcellular entities with independent structures and functions.
Some enzyme complexes built inside microbial cells can be isolated as independent entities and catalyze biosynthetic reactions ex vivo, motivating the term synthetic organelles.
Enzyme complexes constructed inside microbial cells can be separated as independent entities and catalyze biosynthetic reactions ex vivo; such a feature gains these complexes another name, "synthetic organelles" - new subcellular entities with independent structures and functions.
Some enzyme complexes built inside microbial cells can be isolated as independent entities and catalyze biosynthetic reactions ex vivo, motivating the term synthetic organelles.
Enzyme complexes constructed inside microbial cells can be separated as independent entities and catalyze biosynthetic reactions ex vivo; such a feature gains these complexes another name, "synthetic organelles" - new subcellular entities with independent structures and functions.
Some enzyme complexes built inside microbial cells can be isolated as independent entities and catalyze biosynthetic reactions ex vivo, motivating the term synthetic organelles.
Enzyme complexes constructed inside microbial cells can be separated as independent entities and catalyze biosynthetic reactions ex vivo; such a feature gains these complexes another name, "synthetic organelles" - new subcellular entities with independent structures and functions.
Some enzyme complexes built inside microbial cells can be isolated as independent entities and catalyze biosynthetic reactions ex vivo, motivating the term synthetic organelles.
Enzyme complexes constructed inside microbial cells can be separated as independent entities and catalyze biosynthetic reactions ex vivo; such a feature gains these complexes another name, "synthetic organelles" - new subcellular entities with independent structures and functions.
Metabolon-like multienzyme assembly brings sequential enzyme active sites into proximity, promoting intermediate transfer, reducing intermediate leakage, and streamlining metabolic flux toward desired products.
The formation of multienzyme complexes, or metabolons, brings the enzyme active sites into proximity to promote intermediate transfer, decrease intermediate leakage, and streamline the metabolic flux towards the desired products.
Metabolon-like multienzyme assembly brings sequential enzyme active sites into proximity, promoting intermediate transfer, reducing intermediate leakage, and streamlining metabolic flux toward desired products.
The formation of multienzyme complexes, or metabolons, brings the enzyme active sites into proximity to promote intermediate transfer, decrease intermediate leakage, and streamline the metabolic flux towards the desired products.
Metabolon-like multienzyme assembly brings sequential enzyme active sites into proximity, promoting intermediate transfer, reducing intermediate leakage, and streamlining metabolic flux toward desired products.
The formation of multienzyme complexes, or metabolons, brings the enzyme active sites into proximity to promote intermediate transfer, decrease intermediate leakage, and streamline the metabolic flux towards the desired products.
Metabolon-like multienzyme assembly brings sequential enzyme active sites into proximity, promoting intermediate transfer, reducing intermediate leakage, and streamlining metabolic flux toward desired products.
The formation of multienzyme complexes, or metabolons, brings the enzyme active sites into proximity to promote intermediate transfer, decrease intermediate leakage, and streamline the metabolic flux towards the desired products.
Metabolon-like multienzyme assembly brings sequential enzyme active sites into proximity, promoting intermediate transfer, reducing intermediate leakage, and streamlining metabolic flux toward desired products.
The formation of multienzyme complexes, or metabolons, brings the enzyme active sites into proximity to promote intermediate transfer, decrease intermediate leakage, and streamline the metabolic flux towards the desired products.
Metabolon-like multienzyme assembly brings sequential enzyme active sites into proximity, promoting intermediate transfer, reducing intermediate leakage, and streamlining metabolic flux toward desired products.
The formation of multienzyme complexes, or metabolons, brings the enzyme active sites into proximity to promote intermediate transfer, decrease intermediate leakage, and streamline the metabolic flux towards the desired products.
Metabolon-like multienzyme assembly brings sequential enzyme active sites into proximity, promoting intermediate transfer, reducing intermediate leakage, and streamlining metabolic flux toward desired products.
The formation of multienzyme complexes, or metabolons, brings the enzyme active sites into proximity to promote intermediate transfer, decrease intermediate leakage, and streamline the metabolic flux towards the desired products.
Enzyme complexation can optimize metabolic flux inside microbes, increase product titer, and provide high-yield microbial strains amenable to large-scale fermentation.
Enzyme complexation optimizes the metabolic fluxes inside microbes, increases the product titer, and supplies the field with high-yield microbe strains that are amenable to large-scale fermentation.
Enzyme complexation can optimize metabolic flux inside microbes, increase product titer, and provide high-yield microbial strains amenable to large-scale fermentation.
Enzyme complexation optimizes the metabolic fluxes inside microbes, increases the product titer, and supplies the field with high-yield microbe strains that are amenable to large-scale fermentation.
Enzyme complexation can optimize metabolic flux inside microbes, increase product titer, and provide high-yield microbial strains amenable to large-scale fermentation.
Enzyme complexation optimizes the metabolic fluxes inside microbes, increases the product titer, and supplies the field with high-yield microbe strains that are amenable to large-scale fermentation.
Enzyme complexation can optimize metabolic flux inside microbes, increase product titer, and provide high-yield microbial strains amenable to large-scale fermentation.
Enzyme complexation optimizes the metabolic fluxes inside microbes, increases the product titer, and supplies the field with high-yield microbe strains that are amenable to large-scale fermentation.
Enzyme complexation can optimize metabolic flux inside microbes, increase product titer, and provide high-yield microbial strains amenable to large-scale fermentation.
Enzyme complexation optimizes the metabolic fluxes inside microbes, increases the product titer, and supplies the field with high-yield microbe strains that are amenable to large-scale fermentation.
Enzyme complexation can optimize metabolic flux inside microbes, increase product titer, and provide high-yield microbial strains amenable to large-scale fermentation.
Enzyme complexation optimizes the metabolic fluxes inside microbes, increases the product titer, and supplies the field with high-yield microbe strains that are amenable to large-scale fermentation.
Enzyme complexation can optimize metabolic flux inside microbes, increase product titer, and provide high-yield microbial strains amenable to large-scale fermentation.
Enzyme complexation optimizes the metabolic fluxes inside microbes, increases the product titer, and supplies the field with high-yield microbe strains that are amenable to large-scale fermentation.
Synthetic versions of metabolons have been developed through multiple strategies to enhance catalytic rates for synthesis of valuable chemicals inside microbes.
We and others have developed synthetic versions of metabolons through various strategies to enhance the catalytic rates for synthesizing valuable chemicals inside microbes.
Synthetic versions of metabolons have been developed through multiple strategies to enhance catalytic rates for synthesis of valuable chemicals inside microbes.
We and others have developed synthetic versions of metabolons through various strategies to enhance the catalytic rates for synthesizing valuable chemicals inside microbes.
Synthetic versions of metabolons have been developed through multiple strategies to enhance catalytic rates for synthesis of valuable chemicals inside microbes.
We and others have developed synthetic versions of metabolons through various strategies to enhance the catalytic rates for synthesizing valuable chemicals inside microbes.
Synthetic versions of metabolons have been developed through multiple strategies to enhance catalytic rates for synthesis of valuable chemicals inside microbes.
We and others have developed synthetic versions of metabolons through various strategies to enhance the catalytic rates for synthesizing valuable chemicals inside microbes.
Synthetic versions of metabolons have been developed through multiple strategies to enhance catalytic rates for synthesis of valuable chemicals inside microbes.
We and others have developed synthetic versions of metabolons through various strategies to enhance the catalytic rates for synthesizing valuable chemicals inside microbes.
Synthetic versions of metabolons have been developed through multiple strategies to enhance catalytic rates for synthesis of valuable chemicals inside microbes.
We and others have developed synthetic versions of metabolons through various strategies to enhance the catalytic rates for synthesizing valuable chemicals inside microbes.
Synthetic versions of metabolons have been developed through multiple strategies to enhance catalytic rates for synthesis of valuable chemicals inside microbes.
We and others have developed synthetic versions of metabolons through various strategies to enhance the catalytic rates for synthesizing valuable chemicals inside microbes.
The field still needs strategies that better balance dynamicity and confinement and provide finer control of local compartmentalization in cells.
Still, the field is seeking new strategies to better balance dynamicity and confinement and to achieve finer control of local compartmentalization in the cells, as the natural multienzyme complexes do.
The field still needs strategies that better balance dynamicity and confinement and provide finer control of local compartmentalization in cells.
Still, the field is seeking new strategies to better balance dynamicity and confinement and to achieve finer control of local compartmentalization in the cells, as the natural multienzyme complexes do.
The field still needs strategies that better balance dynamicity and confinement and provide finer control of local compartmentalization in cells.
Still, the field is seeking new strategies to better balance dynamicity and confinement and to achieve finer control of local compartmentalization in the cells, as the natural multienzyme complexes do.
The field still needs strategies that better balance dynamicity and confinement and provide finer control of local compartmentalization in cells.
Still, the field is seeking new strategies to better balance dynamicity and confinement and to achieve finer control of local compartmentalization in the cells, as the natural multienzyme complexes do.
The field still needs strategies that better balance dynamicity and confinement and provide finer control of local compartmentalization in cells.
Still, the field is seeking new strategies to better balance dynamicity and confinement and to achieve finer control of local compartmentalization in the cells, as the natural multienzyme complexes do.
The field still needs strategies that better balance dynamicity and confinement and provide finer control of local compartmentalization in cells.
Still, the field is seeking new strategies to better balance dynamicity and confinement and to achieve finer control of local compartmentalization in the cells, as the natural multienzyme complexes do.
The field still needs strategies that better balance dynamicity and confinement and provide finer control of local compartmentalization in cells.
Still, the field is seeking new strategies to better balance dynamicity and confinement and to achieve finer control of local compartmentalization in the cells, as the natural multienzyme complexes do.
Industrial applications of synthetic multienzyme complexes for large-scale production of valuable chemicals have not yet been realized.
Industrial applications of synthetic multienzyme complexes for the large-scale production of valuable chemicals are yet to be realized.
Industrial applications of synthetic multienzyme complexes for large-scale production of valuable chemicals have not yet been realized.
Industrial applications of synthetic multienzyme complexes for the large-scale production of valuable chemicals are yet to be realized.
Industrial applications of synthetic multienzyme complexes for large-scale production of valuable chemicals have not yet been realized.
Industrial applications of synthetic multienzyme complexes for the large-scale production of valuable chemicals are yet to be realized.
Industrial applications of synthetic multienzyme complexes for large-scale production of valuable chemicals have not yet been realized.
Industrial applications of synthetic multienzyme complexes for the large-scale production of valuable chemicals are yet to be realized.
Industrial applications of synthetic multienzyme complexes for large-scale production of valuable chemicals have not yet been realized.
Industrial applications of synthetic multienzyme complexes for the large-scale production of valuable chemicals are yet to be realized.
Industrial applications of synthetic multienzyme complexes for large-scale production of valuable chemicals have not yet been realized.
Industrial applications of synthetic multienzyme complexes for the large-scale production of valuable chemicals are yet to be realized.
Industrial applications of synthetic multienzyme complexes for large-scale production of valuable chemicals have not yet been realized.
Industrial applications of synthetic multienzyme complexes for the large-scale production of valuable chemicals are yet to be realized.
Approval Evidence
1 source5 linked approval claimsfirst-pass slug synthetic-multienzyme-complexes
Synthetic multienzyme complexes range from static enzyme nanostructures to dynamic enzyme coacervates.
Source:
functional capabilitysupports
Some enzyme complexes built inside microbial cells can be isolated as independent entities and catalyze biosynthetic reactions ex vivo, motivating the term synthetic organelles.
Enzyme complexes constructed inside microbial cells can be separated as independent entities and catalyze biosynthetic reactions ex vivo; such a feature gains these complexes another name, "synthetic organelles" - new subcellular entities with independent structures and functions.
Source:
performance summarysupports
Enzyme complexation can optimize metabolic flux inside microbes, increase product titer, and provide high-yield microbial strains amenable to large-scale fermentation.
Enzyme complexation optimizes the metabolic fluxes inside microbes, increases the product titer, and supplies the field with high-yield microbe strains that are amenable to large-scale fermentation.
Source:
performance summarysupports
Synthetic versions of metabolons have been developed through multiple strategies to enhance catalytic rates for synthesis of valuable chemicals inside microbes.
We and others have developed synthetic versions of metabolons through various strategies to enhance the catalytic rates for synthesizing valuable chemicals inside microbes.
Source:
research gapsupports
The field still needs strategies that better balance dynamicity and confinement and provide finer control of local compartmentalization in cells.
Still, the field is seeking new strategies to better balance dynamicity and confinement and to achieve finer control of local compartmentalization in the cells, as the natural multienzyme complexes do.
Source:
translation gapsupports
Industrial applications of synthetic multienzyme complexes for large-scale production of valuable chemicals have not yet been realized.
Industrial applications of synthetic multienzyme complexes for the large-scale production of valuable chemicals are yet to be realized.
Source:
Comparisons
Source-backed strengths
The available evidence supports two key strengths: enzyme assemblies can be configured in both static nanostructured and dynamic coacervate formats, and some intracellularly built complexes can be isolated as independent catalytic entities for ex vivo reactions. The concept is explicitly framed as enabling metabolon-like proximity effects that favor intermediate transfer and reduce leakage.
Source:
Enzyme complexation optimizes the metabolic fluxes inside microbes, increases the product titer, and supplies the field with high-yield microbe strains that are amenable to large-scale fermentation.
Source:
We and others have developed synthetic versions of metabolons through various strategies to enhance the catalytic rates for synthesizing valuable chemicals inside microbes.
Compared with H-2Kb-ADSCs
synthetic multienzyme complexes and H-2Kb-ADSCs address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination; same primary input modality: chemical
Compared with inkube
synthetic multienzyme complexes and inkube address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination; same primary input modality: chemical
Compared with metabolons
synthetic multienzyme complexes and metabolons address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination; shared mechanisms: enzyme colocalization; same primary input modality: chemical
Ranked Citations
- 1.