Source 1primary paper2018ChemBioChem
Toolkit/TA system
TA system
Also known as: transcription activation (TA)
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The TA system is a BphP1-QPAS1-based near-infrared light-sensing optogenetic system for transcription activation. It was tested in several mammalian cell types, including cortical neurons, and is described within a study of near-infrared-controlled gene expression and protein targeting.
Usefulness & Problems
Why this is useful
This system provides optical control of transcription using near-infrared light in mammalian cells, including neurons. The small size of QPAS1 enabled AAV particle design for neuronal delivery, supporting use in contexts where vector payload is a practical constraint.
Source:
Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
Problem solved
The TA system addresses the need for a near-infrared-responsive optogenetic method to activate transcription in mammalian cells. It also helps address delivery constraints for neuronal applications because the QPAS1 component is small enough to support AAV-based design.
Source:
Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Techniques
Computational DesignTarget processes
localizationtranscriptionInput: Light
Implementation Constraints
The system is based on the BphP1-QPAS1 optogenetic pair and is actuated by near-infrared light. Evidence indicates that QPAS1 is sufficiently small to enable AAV particle design for delivery of the TA system to neurons, but the supplied evidence does not specify construct architecture or illumination parameters.
Reported performance of BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, including nuclear transport. The available evidence here does not provide quantitative performance metrics, dynamic range, or direct independent replication specific to TA.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.
Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.
Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.
Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.
Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.
Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.
Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.
Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.
We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.
We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.
We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.
We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.
We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.
We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.
We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
The small size of QPAS1 enabled design of AAV particles for delivery of the TA system to neurons.
The small size of the QPAS1 component allowed the design of adeno-associated virus (AAV) particles, which were applied to deliver the TA system to neurons.
The small size of QPAS1 enabled design of AAV particles for delivery of the TA system to neurons.
The small size of the QPAS1 component allowed the design of adeno-associated virus (AAV) particles, which were applied to deliver the TA system to neurons.
The small size of QPAS1 enabled design of AAV particles for delivery of the TA system to neurons.
The small size of the QPAS1 component allowed the design of adeno-associated virus (AAV) particles, which were applied to deliver the TA system to neurons.
The small size of QPAS1 enabled design of AAV particles for delivery of the TA system to neurons.
The small size of the QPAS1 component allowed the design of adeno-associated virus (AAV) particles, which were applied to deliver the TA system to neurons.
The small size of QPAS1 enabled design of AAV particles for delivery of the TA system to neurons.
The small size of the QPAS1 component allowed the design of adeno-associated virus (AAV) particles, which were applied to deliver the TA system to neurons.
The small size of QPAS1 enabled design of AAV particles for delivery of the TA system to neurons.
The small size of the QPAS1 component allowed the design of adeno-associated virus (AAV) particles, which were applied to deliver the TA system to neurons.
The small size of QPAS1 enabled design of AAV particles for delivery of the TA system to neurons.
The small size of the QPAS1 component allowed the design of adeno-associated virus (AAV) particles, which were applied to deliver the TA system to neurons.
The NIR-light-sensing component of iRIS performed well in all tested cell types.
In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
The NIR-light-sensing component of iRIS performed well in all tested cell types.
In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
The NIR-light-sensing component of iRIS performed well in all tested cell types.
In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
The NIR-light-sensing component of iRIS performed well in all tested cell types.
In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
The NIR-light-sensing component of iRIS performed well in all tested cell types.
In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
The NIR-light-sensing component of iRIS performed well in all tested cell types.
In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
The NIR-light-sensing component of iRIS performed well in all tested cell types.
In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
The TA system showed the best performance in HeLa, U-2 OS, and HEK-293 cells.
The TA system showed the best performance in cervical cancer (HeLa), bone cancer (U-2 OS), and human embryonic kidney (HEK-293) cells.
The TA system showed the best performance in HeLa, U-2 OS, and HEK-293 cells.
The TA system showed the best performance in cervical cancer (HeLa), bone cancer (U-2 OS), and human embryonic kidney (HEK-293) cells.
The TA system showed the best performance in HeLa, U-2 OS, and HEK-293 cells.
The TA system showed the best performance in cervical cancer (HeLa), bone cancer (U-2 OS), and human embryonic kidney (HEK-293) cells.
The TA system showed the best performance in HeLa, U-2 OS, and HEK-293 cells.
The TA system showed the best performance in cervical cancer (HeLa), bone cancer (U-2 OS), and human embryonic kidney (HEK-293) cells.
The TA system showed the best performance in HeLa, U-2 OS, and HEK-293 cells.
The TA system showed the best performance in cervical cancer (HeLa), bone cancer (U-2 OS), and human embryonic kidney (HEK-293) cells.
The TA system showed the best performance in HeLa, U-2 OS, and HEK-293 cells.
The TA system showed the best performance in cervical cancer (HeLa), bone cancer (U-2 OS), and human embryonic kidney (HEK-293) cells.
The TA system showed the best performance in HeLa, U-2 OS, and HEK-293 cells.
The TA system showed the best performance in cervical cancer (HeLa), bone cancer (U-2 OS), and human embryonic kidney (HEK-293) cells.
Approval Evidence
1 source4 linked approval claimsfirst-pass slug ta-system
an NIR light-sensing system for transcription activation (TA)
Source:
application scopesupports
The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.
Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
Source:
cell type dependencesupports
Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.
We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
Source:
delivery enabling propertysupports
The small size of QPAS1 enabled design of AAV particles for delivery of the TA system to neurons.
The small size of the QPAS1 component allowed the design of adeno-associated virus (AAV) particles, which were applied to deliver the TA system to neurons.
Source:
performancesupports
The TA system showed the best performance in HeLa, U-2 OS, and HEK-293 cells.
The TA system showed the best performance in cervical cancer (HeLa), bone cancer (U-2 OS), and human embryonic kidney (HEK-293) cells.
Source:
Comparisons
Source-backed strengths
The tool was experimentally tested in several mammalian cell types, including cortical neurons. Its use of the BphP1-QPAS1 pair and the small size of QPAS1 supported AAV design for neuronal delivery.
Source:
In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
Source:
The TA system showed the best performance in cervical cancer (HeLa), bone cancer (U-2 OS), and human embryonic kidney (HEK-293) cells.
Ranked Citations
- 1.