Source 1primary paper2015Angewandte Chemie International Edition
Toolkit/two-photon-sensitive caging group for gibberellic acid activation
two-photon-sensitive caging group for gibberellic acid activation
Also known as: two-photon (2P)-sensitive caging group
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
This tool is a two-photon-sensitive chemical caging group applied to gibberellic acid 3 (GA3) to permit infrared-light-triggered release of active GA3 in living cells. The uncaged GA3 is used to induce protein dimerization.
Usefulness & Problems
Why this is useful
The tool enables optical control of GA3-dependent protein dimerization using infrared light in living cells. It is useful where two-photon activation is desired for light-dependent control of intracellular signaling or protein association.
Problem solved
It addresses the problem of activating gibberellic acid with light in living cells using a two-photon-sensitive strategy rather than only one-photon photolysis. The reported caging group provides a way to efficiently release active GA3 with infrared illumination.
Problem links
Need precise spatiotemporal control with light input
DerivedThis tool is a two-photon-sensitive caging group applied to gibberellic acid 3 (GA3) to enable infrared-light-dependent release of active GA3 in living cells. The released GA3 is used to induce protein dimerization.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
HeterodimerizationHeterodimerizationHeterodimerizationphotocaging/photouncagingphotocaging/photouncagingTechniques
No technique tags yet.
Target processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: multi component delivery burdenimplementation constraint: spectral hardware requirementoperating role: actuatorswitch architecture: multi componentswitch architecture: recruitmentswitch architecture: uncaging
Implementation requires a GA3 derivative bearing the two-photon-sensitive caging group and infrared light for activation. The available evidence supports use in living cells, but it does not provide construct design details, delivery conditions, or illumination parameters.
The provided evidence does not specify the caging group structure, activation wavelength, uncaging kinetics, or quantitative dimerization performance. Independent replication and validation beyond the cited study are not documented in the supplied evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
A new two-photon-sensitive caging group allows infrared-light activation of gibberellic acid to induce protein dimerization in living cells.
we present a new two-photon (2P)-sensitive caging group, whose exceptionally high two-photon cross section allows the use of infrared light to efficiently unleash the active GA3 for inducing protein dimerization in living cells.
two-photon cross section exceptionally high
A new two-photon-sensitive caging group allows infrared-light activation of gibberellic acid to induce protein dimerization in living cells.
we present a new two-photon (2P)-sensitive caging group, whose exceptionally high two-photon cross section allows the use of infrared light to efficiently unleash the active GA3 for inducing protein dimerization in living cells.
two-photon cross section exceptionally high
A new two-photon-sensitive caging group allows infrared-light activation of gibberellic acid to induce protein dimerization in living cells.
we present a new two-photon (2P)-sensitive caging group, whose exceptionally high two-photon cross section allows the use of infrared light to efficiently unleash the active GA3 for inducing protein dimerization in living cells.
two-photon cross section exceptionally high
A new two-photon-sensitive caging group allows infrared-light activation of gibberellic acid to induce protein dimerization in living cells.
we present a new two-photon (2P)-sensitive caging group, whose exceptionally high two-photon cross section allows the use of infrared light to efficiently unleash the active GA3 for inducing protein dimerization in living cells.
two-photon cross section exceptionally high
A new two-photon-sensitive caging group allows infrared-light activation of gibberellic acid to induce protein dimerization in living cells.
we present a new two-photon (2P)-sensitive caging group, whose exceptionally high two-photon cross section allows the use of infrared light to efficiently unleash the active GA3 for inducing protein dimerization in living cells.
two-photon cross section exceptionally high
A new two-photon-sensitive caging group allows infrared-light activation of gibberellic acid to induce protein dimerization in living cells.
we present a new two-photon (2P)-sensitive caging group, whose exceptionally high two-photon cross section allows the use of infrared light to efficiently unleash the active GA3 for inducing protein dimerization in living cells.
two-photon cross section exceptionally high
A new two-photon-sensitive caging group allows infrared-light activation of gibberellic acid to induce protein dimerization in living cells.
we present a new two-photon (2P)-sensitive caging group, whose exceptionally high two-photon cross section allows the use of infrared light to efficiently unleash the active GA3 for inducing protein dimerization in living cells.
two-photon cross section exceptionally high
A new two-photon-sensitive caging group allows infrared-light activation of gibberellic acid to induce protein dimerization in living cells.
we present a new two-photon (2P)-sensitive caging group, whose exceptionally high two-photon cross section allows the use of infrared light to efficiently unleash the active GA3 for inducing protein dimerization in living cells.
two-photon cross section exceptionally high
A new two-photon-sensitive caging group allows infrared-light activation of gibberellic acid to induce protein dimerization in living cells.
we present a new two-photon (2P)-sensitive caging group, whose exceptionally high two-photon cross section allows the use of infrared light to efficiently unleash the active GA3 for inducing protein dimerization in living cells.
two-photon cross section exceptionally high
A new two-photon-sensitive caging group allows infrared-light activation of gibberellic acid to induce protein dimerization in living cells.
we present a new two-photon (2P)-sensitive caging group, whose exceptionally high two-photon cross section allows the use of infrared light to efficiently unleash the active GA3 for inducing protein dimerization in living cells.
two-photon cross section exceptionally high
A new two-photon-sensitive caging group allows infrared-light activation of gibberellic acid to induce protein dimerization in living cells.
we present a new two-photon (2P)-sensitive caging group, whose exceptionally high two-photon cross section allows the use of infrared light to efficiently unleash the active GA3 for inducing protein dimerization in living cells.
two-photon cross section exceptionally high
A new two-photon-sensitive caging group allows infrared-light activation of gibberellic acid to induce protein dimerization in living cells.
we present a new two-photon (2P)-sensitive caging group, whose exceptionally high two-photon cross section allows the use of infrared light to efficiently unleash the active GA3 for inducing protein dimerization in living cells.
two-photon cross section exceptionally high
A new two-photon-sensitive caging group allows infrared-light activation of gibberellic acid to induce protein dimerization in living cells.
we present a new two-photon (2P)-sensitive caging group, whose exceptionally high two-photon cross section allows the use of infrared light to efficiently unleash the active GA3 for inducing protein dimerization in living cells.
two-photon cross section exceptionally high
A new two-photon-sensitive caging group allows infrared-light activation of gibberellic acid to induce protein dimerization in living cells.
we present a new two-photon (2P)-sensitive caging group, whose exceptionally high two-photon cross section allows the use of infrared light to efficiently unleash the active GA3 for inducing protein dimerization in living cells.
two-photon cross section exceptionally high
A new two-photon-sensitive caging group allows infrared-light activation of gibberellic acid to induce protein dimerization in living cells.
we present a new two-photon (2P)-sensitive caging group, whose exceptionally high two-photon cross section allows the use of infrared light to efficiently unleash the active GA3 for inducing protein dimerization in living cells.
two-photon cross section exceptionally high
A new two-photon-sensitive caging group allows infrared-light activation of gibberellic acid to induce protein dimerization in living cells.
we present a new two-photon (2P)-sensitive caging group, whose exceptionally high two-photon cross section allows the use of infrared light to efficiently unleash the active GA3 for inducing protein dimerization in living cells.
two-photon cross section exceptionally high
A new two-photon-sensitive caging group allows infrared-light activation of gibberellic acid to induce protein dimerization in living cells.
we present a new two-photon (2P)-sensitive caging group, whose exceptionally high two-photon cross section allows the use of infrared light to efficiently unleash the active GA3 for inducing protein dimerization in living cells.
two-photon cross section exceptionally high
Approval Evidence
1 source1 linked approval claimfirst-pass slug two-photon-sensitive-caging-group-for-gibberellic-acid-activation
we present a new two-photon (2P)-sensitive caging group, whose exceptionally high two-photon cross section allows the use of infrared light to efficiently unleash the active GA3
Source:
photochemical propertysupports
A new two-photon-sensitive caging group allows infrared-light activation of gibberellic acid to induce protein dimerization in living cells.
we present a new two-photon (2P)-sensitive caging group, whose exceptionally high two-photon cross section allows the use of infrared light to efficiently unleash the active GA3 for inducing protein dimerization in living cells.
Source:
Comparisons
Source-backed strengths
The source states that the caging group has an exceptionally high two-photon cross section, supporting efficient two-photon uncaging. It was specifically reported to allow infrared-light activation of GA3 and induction of protein dimerization in living cells.
Compared with LightOn system
two-photon-sensitive caging group for gibberellic acid activation and LightOn system address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: heterodimerization; same primary input modality: light
Compared with photo-activatable Akt probe
two-photon-sensitive caging group for gibberellic acid activation and photo-activatable Akt probe address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: heterodimerization; same primary input modality: light
Compared with tandem-dimer nano (tdnano)
two-photon-sensitive caging group for gibberellic acid activation and tandem-dimer nano (tdnano) address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: heterodimerization; same primary input modality: light
Ranked Citations
- 1.