Source 1primary paper2024
Toolkit/ultra-widefield microscopy platform for parallel light patterning
ultra-widefield microscopy platform for parallel light patterning
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
This assay method is an adapted ultra-widefield microscopy platform used for parallel light patterning in up to 36 embryos. It enables precise spatial control of optogenetically driven Nodal signaling activity and downstream gene expression in developmental experiments.
Usefulness & Problems
Why this is useful
The platform is useful for imposing defined spatiotemporal light inputs across many embryos in parallel while monitoring developmental responses. In the cited study, it supported patterned Nodal activation experiments that controlled endodermal precursor internalization and generated synthetic signaling patterns.
Source:
The improved optoNodal2 reagents eliminate dark activity and improve response kinetics, without sacrificing dynamic range.
Problem solved
It addresses the experimental problem of delivering reproducible, spatially patterned optogenetic stimulation across multiple embryos at once during development. The reported application was control of Nodal signaling patterns and associated downstream gene expression in embryos, including mutant rescue experiments.
Problem links
Need conditional control of signaling activity
DerivedThis assay method is an adapted ultra-widefield microscopy platform used for parallel light patterning in up to 36 embryos. It enables precise spatial control of optogenetically driven Nodal signaling activity and downstream gene expression in developmental experiments.
Need precise spatiotemporal control with light input
DerivedThis assay method is an adapted ultra-widefield microscopy platform used for parallel light patterning in up to 36 embryos. It enables precise spatial control of optogenetically driven Nodal signaling activity and downstream gene expression in developmental experiments.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
optogenetic control of signalingoptogenetic control of signalingspatiotemporal light patterningspatiotemporal light patterningTechniques
Functional AssayTarget processes
signalingInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensor
Implementation involved adaptation of an ultra-widefield microscopy platform for parallel light patterning in embryos. The supplied evidence supports use with optogenetic control of Nodal signaling, but does not specify optical components, construct design, embryo species, or illumination parameters.
The available evidence describes the platform's use in one developmental optogenetics study and does not provide broader benchmarking across organisms, signaling systems, or imaging modalities. Quantitative performance details such as spatial resolution, temporal resolution, illumination wavelengths, and hardware specifications are not provided in the supplied evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Patterned Nodal activation drove precisely controlled internalization of endodermal precursors.
Patterned Nodal activation drove precisely controlled internalization of endodermal precursors.
Patterned Nodal activation drove precisely controlled internalization of endodermal precursors.
Patterned Nodal activation drove precisely controlled internalization of endodermal precursors.
Patterned Nodal activation drove precisely controlled internalization of endodermal precursors.
Patterned Nodal activation drove precisely controlled internalization of endodermal precursors.
Patterned Nodal activation drove precisely controlled internalization of endodermal precursors.
Patterned Nodal activation drove precisely controlled internalization of endodermal precursors.
Patterned Nodal activation drove precisely controlled internalization of endodermal precursors.
Patterned Nodal activation drove precisely controlled internalization of endodermal precursors.
Patterned Nodal activation drove precisely controlled internalization of endodermal precursors.
Patterned Nodal activation drove precisely controlled internalization of endodermal precursors.
Patterned Nodal activation drove precisely controlled internalization of endodermal precursors.
Patterned Nodal activation drove precisely controlled internalization of endodermal precursors.
Patterned Nodal activation drove precisely controlled internalization of endodermal precursors.
Patterned Nodal activation drove precisely controlled internalization of endodermal precursors.
Patterned Nodal activation drove precisely controlled internalization of endodermal precursors.
Patterned Nodal activation drove precisely controlled internalization of endodermal precursors.
Patterned Nodal activation drove precisely controlled internalization of endodermal precursors.
Patterned Nodal activation drove precisely controlled internalization of endodermal precursors.
Patterned illumination generated synthetic signaling patterns in Nodal signaling mutants and rescued several characteristic developmental defects.
we used patterned illumination to generate synthetic signaling patterns in Nodal signaling mutants, rescuing several characteristic developmental defects
Patterned illumination generated synthetic signaling patterns in Nodal signaling mutants and rescued several characteristic developmental defects.
we used patterned illumination to generate synthetic signaling patterns in Nodal signaling mutants, rescuing several characteristic developmental defects
Patterned illumination generated synthetic signaling patterns in Nodal signaling mutants and rescued several characteristic developmental defects.
we used patterned illumination to generate synthetic signaling patterns in Nodal signaling mutants, rescuing several characteristic developmental defects
Patterned illumination generated synthetic signaling patterns in Nodal signaling mutants and rescued several characteristic developmental defects.
we used patterned illumination to generate synthetic signaling patterns in Nodal signaling mutants, rescuing several characteristic developmental defects
Patterned illumination generated synthetic signaling patterns in Nodal signaling mutants and rescued several characteristic developmental defects.
we used patterned illumination to generate synthetic signaling patterns in Nodal signaling mutants, rescuing several characteristic developmental defects
Patterned illumination generated synthetic signaling patterns in Nodal signaling mutants and rescued several characteristic developmental defects.
we used patterned illumination to generate synthetic signaling patterns in Nodal signaling mutants, rescuing several characteristic developmental defects
Patterned illumination generated synthetic signaling patterns in Nodal signaling mutants and rescued several characteristic developmental defects.
we used patterned illumination to generate synthetic signaling patterns in Nodal signaling mutants, rescuing several characteristic developmental defects
Patterned illumination generated synthetic signaling patterns in Nodal signaling mutants and rescued several characteristic developmental defects.
we used patterned illumination to generate synthetic signaling patterns in Nodal signaling mutants, rescuing several characteristic developmental defects
Patterned illumination generated synthetic signaling patterns in Nodal signaling mutants and rescued several characteristic developmental defects.
we used patterned illumination to generate synthetic signaling patterns in Nodal signaling mutants, rescuing several characteristic developmental defects
Patterned illumination generated synthetic signaling patterns in Nodal signaling mutants and rescued several characteristic developmental defects.
we used patterned illumination to generate synthetic signaling patterns in Nodal signaling mutants, rescuing several characteristic developmental defects
An adapted ultra-widefield microscopy platform enabled parallel light patterning in up to 36 embryos and precise spatial control over Nodal signaling activity and downstream gene expression.
We adapted an ultra-widefield microscopy platform for parallel light patterning in up to 36 embryos and demonstrated precise spatial control over Nodal signaling activity and downstream gene expression.
parallel light patterned embryos 36 embryos
An adapted ultra-widefield microscopy platform enabled parallel light patterning in up to 36 embryos and precise spatial control over Nodal signaling activity and downstream gene expression.
We adapted an ultra-widefield microscopy platform for parallel light patterning in up to 36 embryos and demonstrated precise spatial control over Nodal signaling activity and downstream gene expression.
parallel light patterned embryos 36 embryos
An adapted ultra-widefield microscopy platform enabled parallel light patterning in up to 36 embryos and precise spatial control over Nodal signaling activity and downstream gene expression.
We adapted an ultra-widefield microscopy platform for parallel light patterning in up to 36 embryos and demonstrated precise spatial control over Nodal signaling activity and downstream gene expression.
parallel light patterned embryos 36 embryos
An adapted ultra-widefield microscopy platform enabled parallel light patterning in up to 36 embryos and precise spatial control over Nodal signaling activity and downstream gene expression.
We adapted an ultra-widefield microscopy platform for parallel light patterning in up to 36 embryos and demonstrated precise spatial control over Nodal signaling activity and downstream gene expression.
parallel light patterned embryos 36 embryos
An adapted ultra-widefield microscopy platform enabled parallel light patterning in up to 36 embryos and precise spatial control over Nodal signaling activity and downstream gene expression.
We adapted an ultra-widefield microscopy platform for parallel light patterning in up to 36 embryos and demonstrated precise spatial control over Nodal signaling activity and downstream gene expression.
parallel light patterned embryos 36 embryos
An adapted ultra-widefield microscopy platform enabled parallel light patterning in up to 36 embryos and precise spatial control over Nodal signaling activity and downstream gene expression.
We adapted an ultra-widefield microscopy platform for parallel light patterning in up to 36 embryos and demonstrated precise spatial control over Nodal signaling activity and downstream gene expression.
parallel light patterned embryos 36 embryos
An adapted ultra-widefield microscopy platform enabled parallel light patterning in up to 36 embryos and precise spatial control over Nodal signaling activity and downstream gene expression.
We adapted an ultra-widefield microscopy platform for parallel light patterning in up to 36 embryos and demonstrated precise spatial control over Nodal signaling activity and downstream gene expression.
parallel light patterned embryos 36 embryos
An adapted ultra-widefield microscopy platform enabled parallel light patterning in up to 36 embryos and precise spatial control over Nodal signaling activity and downstream gene expression.
We adapted an ultra-widefield microscopy platform for parallel light patterning in up to 36 embryos and demonstrated precise spatial control over Nodal signaling activity and downstream gene expression.
parallel light patterned embryos 36 embryos
An adapted ultra-widefield microscopy platform enabled parallel light patterning in up to 36 embryos and precise spatial control over Nodal signaling activity and downstream gene expression.
We adapted an ultra-widefield microscopy platform for parallel light patterning in up to 36 embryos and demonstrated precise spatial control over Nodal signaling activity and downstream gene expression.
parallel light patterned embryos 36 embryos
An adapted ultra-widefield microscopy platform enabled parallel light patterning in up to 36 embryos and precise spatial control over Nodal signaling activity and downstream gene expression.
We adapted an ultra-widefield microscopy platform for parallel light patterning in up to 36 embryos and demonstrated precise spatial control over Nodal signaling activity and downstream gene expression.
parallel light patterned embryos 36 embryos
An adapted ultra-widefield microscopy platform enabled parallel light patterning in up to 36 embryos and precise spatial control over Nodal signaling activity and downstream gene expression.
We adapted an ultra-widefield microscopy platform for parallel light patterning in up to 36 embryos and demonstrated precise spatial control over Nodal signaling activity and downstream gene expression.
parallel light patterned embryos 36 embryos
An adapted ultra-widefield microscopy platform enabled parallel light patterning in up to 36 embryos and precise spatial control over Nodal signaling activity and downstream gene expression.
We adapted an ultra-widefield microscopy platform for parallel light patterning in up to 36 embryos and demonstrated precise spatial control over Nodal signaling activity and downstream gene expression.
parallel light patterned embryos 36 embryos
An adapted ultra-widefield microscopy platform enabled parallel light patterning in up to 36 embryos and precise spatial control over Nodal signaling activity and downstream gene expression.
We adapted an ultra-widefield microscopy platform for parallel light patterning in up to 36 embryos and demonstrated precise spatial control over Nodal signaling activity and downstream gene expression.
parallel light patterned embryos 36 embryos
An adapted ultra-widefield microscopy platform enabled parallel light patterning in up to 36 embryos and precise spatial control over Nodal signaling activity and downstream gene expression.
We adapted an ultra-widefield microscopy platform for parallel light patterning in up to 36 embryos and demonstrated precise spatial control over Nodal signaling activity and downstream gene expression.
parallel light patterned embryos 36 embryos
An adapted ultra-widefield microscopy platform enabled parallel light patterning in up to 36 embryos and precise spatial control over Nodal signaling activity and downstream gene expression.
We adapted an ultra-widefield microscopy platform for parallel light patterning in up to 36 embryos and demonstrated precise spatial control over Nodal signaling activity and downstream gene expression.
parallel light patterned embryos 36 embryos
An adapted ultra-widefield microscopy platform enabled parallel light patterning in up to 36 embryos and precise spatial control over Nodal signaling activity and downstream gene expression.
We adapted an ultra-widefield microscopy platform for parallel light patterning in up to 36 embryos and demonstrated precise spatial control over Nodal signaling activity and downstream gene expression.
parallel light patterned embryos 36 embryos
An adapted ultra-widefield microscopy platform enabled parallel light patterning in up to 36 embryos and precise spatial control over Nodal signaling activity and downstream gene expression.
We adapted an ultra-widefield microscopy platform for parallel light patterning in up to 36 embryos and demonstrated precise spatial control over Nodal signaling activity and downstream gene expression.
parallel light patterned embryos 36 embryos
Improved optoNodal2 reagents eliminate dark activity and improve response kinetics without sacrificing dynamic range.
The improved optoNodal2 reagents eliminate dark activity and improve response kinetics, without sacrificing dynamic range.
Improved optoNodal2 reagents eliminate dark activity and improve response kinetics without sacrificing dynamic range.
The improved optoNodal2 reagents eliminate dark activity and improve response kinetics, without sacrificing dynamic range.
Improved optoNodal2 reagents eliminate dark activity and improve response kinetics without sacrificing dynamic range.
The improved optoNodal2 reagents eliminate dark activity and improve response kinetics, without sacrificing dynamic range.
Improved optoNodal2 reagents eliminate dark activity and improve response kinetics without sacrificing dynamic range.
The improved optoNodal2 reagents eliminate dark activity and improve response kinetics, without sacrificing dynamic range.
Improved optoNodal2 reagents eliminate dark activity and improve response kinetics without sacrificing dynamic range.
The improved optoNodal2 reagents eliminate dark activity and improve response kinetics, without sacrificing dynamic range.
Improved optoNodal2 reagents eliminate dark activity and improve response kinetics without sacrificing dynamic range.
The improved optoNodal2 reagents eliminate dark activity and improve response kinetics, without sacrificing dynamic range.
Improved optoNodal2 reagents eliminate dark activity and improve response kinetics without sacrificing dynamic range.
The improved optoNodal2 reagents eliminate dark activity and improve response kinetics, without sacrificing dynamic range.
Improved optoNodal2 reagents eliminate dark activity and improve response kinetics without sacrificing dynamic range.
The improved optoNodal2 reagents eliminate dark activity and improve response kinetics, without sacrificing dynamic range.
Improved optoNodal2 reagents eliminate dark activity and improve response kinetics without sacrificing dynamic range.
The improved optoNodal2 reagents eliminate dark activity and improve response kinetics, without sacrificing dynamic range.
Improved optoNodal2 reagents eliminate dark activity and improve response kinetics without sacrificing dynamic range.
The improved optoNodal2 reagents eliminate dark activity and improve response kinetics, without sacrificing dynamic range.
Approval Evidence
1 source1 linked approval claimfirst-pass slug ultra-widefield-microscopy-platform-for-parallel-light-patterning
We adapted an ultra-widefield microscopy platform for parallel light patterning in up to 36 embryos
Source:
spatiotemporal controlsupports
An adapted ultra-widefield microscopy platform enabled parallel light patterning in up to 36 embryos and precise spatial control over Nodal signaling activity and downstream gene expression.
We adapted an ultra-widefield microscopy platform for parallel light patterning in up to 36 embryos and demonstrated precise spatial control over Nodal signaling activity and downstream gene expression.
Source:
Comparisons
Source-backed strengths
The reported strength is parallelized light patterning in up to 36 embryos on an adapted ultra-widefield microscopy platform. The study further reports precise spatial control over Nodal signaling activity and downstream gene expression, as well as rescue of several characteristic developmental defects in Nodal signaling mutants.
Compared with cDNA microarray
ultra-widefield microscopy platform for parallel light patterning and cDNA microarray address a similar problem space because they share signaling.
Shared frame: same top-level item type; shared target processes: signaling; same primary input modality: light
Compared with IRAP-pHluorin translocation assay
ultra-widefield microscopy platform for parallel light patterning and IRAP-pHluorin translocation assay address a similar problem space because they share signaling.
Shared frame: same top-level item type; shared target processes: signaling; same primary input modality: light
ultra-widefield microscopy platform for parallel light patterning and light-induced Fourier transform infrared (FTIR) difference spectroscopy address a similar problem space because they share signaling.
Shared frame: same top-level item type; shared target processes: signaling; same primary input modality: light
Ranked Citations
- 1.