Source 1primary paper2014ACS Synthetic Biology
Toolkit/UV-B light-responsive gene switch
UV-B light-responsive gene switch
Also known as: UV-B light-controlled gene expression
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The UV-B light-responsive gene switch is a mammalian transgene expression system activated by UV-B light and used as one component of a multiwavelength optogenetic control framework. In the cited work, it served alongside blue and red/far-red light-responsive switches to support spectrally separated regulation of gene expression in a single mammalian cell culture.
Usefulness & Problems
Why this is useful
This tool is useful for optogenetic control of transgene expression with a distinct light input that can be combined with other wavelength-specific systems. The cited study positions the UV-B-responsive switch as part of an orthogonal multi-input platform for coordinating multiple gene expression programs in mammalian cells.
Source:
Based on this prediction, we developed a blue light-responsive and rapidly reversible expression system.
Problem solved
It helps address the challenge of building multi-light genetic networks in mammalian cells by providing a UV-B-activated expression channel. The source specifically identifies insufficient orthogonality between UV-B and blue light-controlled expression as a bottleneck, indicating that the UV-B switch is central to resolving spectral cross-talk in such systems.
Source:
Based on this prediction, we developed a blue light-responsive and rapidly reversible expression system.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Techniques
No technique tags yet.
Target processes
No target processes tagged yet.
Input: Light
Implementation Constraints
The evidence indicates use in mammalian cell culture as a transgene expression system responsive to UV-B illumination. No construct design details, cofactors, host cell line information, or delivery methods are provided in the supplied material.
The supplied evidence does not describe the molecular architecture, photoreceptor proteins, dynamic range, activation kinetics, reversibility, or UV-B dose requirements of the UV-B switch. Independent validation beyond the cited 2014 study is not provided, and the orthogonality discussion is framed mainly through the need to redesign the blue-light system.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
A lack of orthogonality between UV-B and blue light-controlled gene expression was identified as the bottleneck for integrating these systems into genetic networks.
We identified a lack of orthogonality between UV-B and blue light-controlled gene expression as the bottleneck
A lack of orthogonality between UV-B and blue light-controlled gene expression was identified as the bottleneck for integrating these systems into genetic networks.
We identified a lack of orthogonality between UV-B and blue light-controlled gene expression as the bottleneck
A lack of orthogonality between UV-B and blue light-controlled gene expression was identified as the bottleneck for integrating these systems into genetic networks.
We identified a lack of orthogonality between UV-B and blue light-controlled gene expression as the bottleneck
A lack of orthogonality between UV-B and blue light-controlled gene expression was identified as the bottleneck for integrating these systems into genetic networks.
We identified a lack of orthogonality between UV-B and blue light-controlled gene expression as the bottleneck
A lack of orthogonality between UV-B and blue light-controlled gene expression was identified as the bottleneck for integrating these systems into genetic networks.
We identified a lack of orthogonality between UV-B and blue light-controlled gene expression as the bottleneck
A lack of orthogonality between UV-B and blue light-controlled gene expression was identified as the bottleneck for integrating these systems into genetic networks.
We identified a lack of orthogonality between UV-B and blue light-controlled gene expression as the bottleneck
A lack of orthogonality between UV-B and blue light-controlled gene expression was identified as the bottleneck for integrating these systems into genetic networks.
We identified a lack of orthogonality between UV-B and blue light-controlled gene expression as the bottleneck
A model-based approach indicated the need for a blue light-responsive gene switch that is insensitive to low-intensity light.
employed a model-based approach that identified the need for a blue light-responsive gene switch that is insensitive to low-intensity light
A model-based approach indicated the need for a blue light-responsive gene switch that is insensitive to low-intensity light.
employed a model-based approach that identified the need for a blue light-responsive gene switch that is insensitive to low-intensity light
A model-based approach indicated the need for a blue light-responsive gene switch that is insensitive to low-intensity light.
employed a model-based approach that identified the need for a blue light-responsive gene switch that is insensitive to low-intensity light
A model-based approach indicated the need for a blue light-responsive gene switch that is insensitive to low-intensity light.
employed a model-based approach that identified the need for a blue light-responsive gene switch that is insensitive to low-intensity light
A model-based approach indicated the need for a blue light-responsive gene switch that is insensitive to low-intensity light.
employed a model-based approach that identified the need for a blue light-responsive gene switch that is insensitive to low-intensity light
A model-based approach indicated the need for a blue light-responsive gene switch that is insensitive to low-intensity light.
employed a model-based approach that identified the need for a blue light-responsive gene switch that is insensitive to low-intensity light
A model-based approach indicated the need for a blue light-responsive gene switch that is insensitive to low-intensity light.
employed a model-based approach that identified the need for a blue light-responsive gene switch that is insensitive to low-intensity light
The developed blue light-responsive expression system was used to demonstrate orthogonality among UV-B, blue, and red/far-red light-responsive gene switches in a single mammalian cell culture.
Finally, we employed this expression system to demonstrate orthogonality between UV-B, blue, and red/far-red light-responsive gene switches in a single mammalian cell culture.
The developed blue light-responsive expression system was used to demonstrate orthogonality among UV-B, blue, and red/far-red light-responsive gene switches in a single mammalian cell culture.
Finally, we employed this expression system to demonstrate orthogonality between UV-B, blue, and red/far-red light-responsive gene switches in a single mammalian cell culture.
The developed blue light-responsive expression system was used to demonstrate orthogonality among UV-B, blue, and red/far-red light-responsive gene switches in a single mammalian cell culture.
Finally, we employed this expression system to demonstrate orthogonality between UV-B, blue, and red/far-red light-responsive gene switches in a single mammalian cell culture.
The developed blue light-responsive expression system was used to demonstrate orthogonality among UV-B, blue, and red/far-red light-responsive gene switches in a single mammalian cell culture.
Finally, we employed this expression system to demonstrate orthogonality between UV-B, blue, and red/far-red light-responsive gene switches in a single mammalian cell culture.
The developed blue light-responsive expression system was used to demonstrate orthogonality among UV-B, blue, and red/far-red light-responsive gene switches in a single mammalian cell culture.
Finally, we employed this expression system to demonstrate orthogonality between UV-B, blue, and red/far-red light-responsive gene switches in a single mammalian cell culture.
The developed blue light-responsive expression system was used to demonstrate orthogonality among UV-B, blue, and red/far-red light-responsive gene switches in a single mammalian cell culture.
Finally, we employed this expression system to demonstrate orthogonality between UV-B, blue, and red/far-red light-responsive gene switches in a single mammalian cell culture.
The developed blue light-responsive expression system was used to demonstrate orthogonality among UV-B, blue, and red/far-red light-responsive gene switches in a single mammalian cell culture.
Finally, we employed this expression system to demonstrate orthogonality between UV-B, blue, and red/far-red light-responsive gene switches in a single mammalian cell culture.
The authors developed a blue light-responsive and rapidly reversible expression system.
Based on this prediction, we developed a blue light-responsive and rapidly reversible expression system.
The authors developed a blue light-responsive and rapidly reversible expression system.
Based on this prediction, we developed a blue light-responsive and rapidly reversible expression system.
The authors developed a blue light-responsive and rapidly reversible expression system.
Based on this prediction, we developed a blue light-responsive and rapidly reversible expression system.
The authors developed a blue light-responsive and rapidly reversible expression system.
Based on this prediction, we developed a blue light-responsive and rapidly reversible expression system.
The authors developed a blue light-responsive and rapidly reversible expression system.
Based on this prediction, we developed a blue light-responsive and rapidly reversible expression system.
The authors developed a blue light-responsive and rapidly reversible expression system.
Based on this prediction, we developed a blue light-responsive and rapidly reversible expression system.
The authors developed a blue light-responsive and rapidly reversible expression system.
Based on this prediction, we developed a blue light-responsive and rapidly reversible expression system.
Approval Evidence
1 source2 linked approval claimsfirst-pass slug uv-b-light-responsive-gene-switch
light-responsive transgene expression systems that are activated by UV-B, blue, or red light have been developed
Source:
bottleneck identificationsupports
A lack of orthogonality between UV-B and blue light-controlled gene expression was identified as the bottleneck for integrating these systems into genetic networks.
We identified a lack of orthogonality between UV-B and blue light-controlled gene expression as the bottleneck
Source:
orthogonality demonstrationsupports
The developed blue light-responsive expression system was used to demonstrate orthogonality among UV-B, blue, and red/far-red light-responsive gene switches in a single mammalian cell culture.
Finally, we employed this expression system to demonstrate orthogonality between UV-B, blue, and red/far-red light-responsive gene switches in a single mammalian cell culture.
Source:
Comparisons
Source-backed strengths
The available evidence supports that UV-B-responsive transgene expression had been developed in mammalian cells and was incorporated into a system demonstrating orthogonality with blue and red/far-red light-responsive switches. This establishes utility in multiwavelength gene control, but the provided evidence does not report quantitative performance metrics for the UV-B component alone.
Ranked Citations
- 1.