Source 1primary paper2013Nucleic Acids Research
Toolkit/UVB-inducible expression system
UVB-inducible expression system
Also known as: UVB-responsive split transcription factor
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The UVB-inducible expression system is a UVB-responsive split transcription factor engineered from the Arabidopsis thaliana UVB receptor UVR8 and the COP1 WD40 domain. In mammalian cells, UVB illumination triggers transcriptional activation and provides one wavelength-specific channel for multichromatic gene control.
Usefulness & Problems
Why this is useful
This system is useful as a UVB-gated gene-expression module that can be combined with blue- and red-light-responsive systems for differential optical control of multiple genes. The cited study used this multichromatic framework for mammalian gene expression control and applications to angiogenic signaling processes.
Source:
This system enables UVB-triggered transcriptional activation in mammalian cells using a split transcription factor design. It serves as one wavelength-specific control channel within a multi-chromatic optogenetic toolkit.
Source:
light-inducible gene expression in mammalian cells
Source:
integration into multi-chromatic gene control setups
Problem solved
It addresses the need for a spectrally distinct light-input channel for mammalian synthetic biology. Specifically, it helps solve the problem of controlling multiple genes in the same cell culture with different wavelengths by adding a UVB-responsive transcriptional module.
Source:
It provides a UVB-responsive gene-expression module that can be combined with other light-responsive systems for differential control of multiple genes. This addresses the need for predictable, complementary control channels in mammalian synthetic biology.
Source:
adds a UVB-responsive channel for orthogonal control of gene expression
Published Workflows
Objective: Develop and combine wavelength-specific optogenetic gene control systems to achieve predictable multi-chromatic control of mammalian gene expression and signaling.
Why it works: The workflow combines orthogonal light-responsive control channels so that different wavelengths can independently regulate different gene outputs in the same mammalian cell culture.
light-responsive split transcription factor activationdifferential gene induction by distinct wavelengthssynthetic system designquantitative modelingsystem combination
Stages
- 1.UVB-responsive system design(library_design)
To create a new UVB-inducible control channel for mammalian gene expression.
Selection: Design of a UVB-responsive split transcription factor based on UVR8 and the WD40 domain of COP1
- 2.Mammalian cell characterization of UVB induction(functional_characterization)
To establish that the engineered UVB system functions strongly in mammalian cells.
Selection: Magnitude of UVB-induced gene expression across mammalian cells
- 3.Quantitative parameter determination(secondary_characterization)
To identify critical parameters that govern system behavior.
Selection: Determination of critical system parameters using a quantitative model
- 4.Combination into multi-chromatic control(confirmatory_validation)
To show that the UVB module can be integrated with blue and red systems for multi-chromatic control.
Selection: Ability to differentially express three genes in one mammalian cell culture using different wavelengths
- 5.Angiogenic signaling application(confirmatory_validation)
To demonstrate that the multi-chromatic control system can regulate a biologically relevant signaling process.
Selection: Application of the multi-chromatic system to control angiogenic signaling processes
Steps
- 1.Design UVB-responsive split transcription factorengineered expression system
Create a UVB-inducible gene expression module for mammalian cells.
A UVB-responsive control channel had to be engineered before it could be characterized or combined with other light-responsive systems.
- 2.Measure UVB-induced gene expression across mammalian cellssystem being characterized
Establish inducibility and host-range performance of the UVB-responsive system.
The newly designed system needed functional characterization before broader integration into a multi-color platform.
- 3.Determine critical system parameters using a quantitative modelanalysis method applied to engineered system
Identify critical parameters governing system behavior.
After establishing that the system functions, modeling supports quantitative understanding needed for predictable use and integration.
- 4.Combine UVB, blue, and red light-inducible systems for multi-gene controlcombined control platform
Build a multi-chromatic system capable of differential control of multiple genes in one culture.
The UVB module was combined with existing blue and red systems only after it had been developed and characterized.
- 5.Apply the multi-chromatic system to angiogenic signaling controlsystem being applied
Demonstrate functional control of a biologically relevant signaling process.
After showing multi-gene control, the authors tested whether the platform could control angiogenic signaling processes.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
light-induced protein interactionsplit transcription factor reconstitutiontranscriptional activationTechniques
Computational DesignTarget processes
signalingtranscriptionInput: Light
Implementation Constraints
The construct was designed from Arabidopsis thaliana UVR8 and the WD40 domain of COP1, indicating a multi-component domain-fusion architecture. Reported use requires UVB illumination and mammalian cell expression, but the supplied evidence does not specify promoter architecture, cofactors, delivery method, or exact construct configuration.
The supplied evidence supports function in mammalian cells and in combination with other optical systems, but it does not provide detailed standalone performance metrics for the UVB module. The evidence also does not describe safety, delivery strategy, in vivo use, or therapeutic validation.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Combining the UVB-responsive system with blue and red light-inducible gene control technology enabled multi-chromatic multi-gene control and was applied to angiogenic signaling processes.
By combining this UVB-responsive system with blue and red light-inducible gene control technology, we demonstrate multi-chromatic multi-gene control by differentially expressing three genes in a single cell culture in mammalian cells, and we apply this system for the multi-chromatic control of angiogenic signaling processes.
Combining the UVB-responsive system with blue and red light-inducible gene control technology enabled multi-chromatic multi-gene control and was applied to angiogenic signaling processes.
By combining this UVB-responsive system with blue and red light-inducible gene control technology, we demonstrate multi-chromatic multi-gene control by differentially expressing three genes in a single cell culture in mammalian cells, and we apply this system for the multi-chromatic control of angiogenic signaling processes.
Combining the UVB-responsive system with blue and red light-inducible gene control technology enabled multi-chromatic multi-gene control and was applied to angiogenic signaling processes.
By combining this UVB-responsive system with blue and red light-inducible gene control technology, we demonstrate multi-chromatic multi-gene control by differentially expressing three genes in a single cell culture in mammalian cells, and we apply this system for the multi-chromatic control of angiogenic signaling processes.
Combining the UVB-responsive system with blue and red light-inducible gene control technology enabled multi-chromatic multi-gene control and was applied to angiogenic signaling processes.
By combining this UVB-responsive system with blue and red light-inducible gene control technology, we demonstrate multi-chromatic multi-gene control by differentially expressing three genes in a single cell culture in mammalian cells, and we apply this system for the multi-chromatic control of angiogenic signaling processes.
Combining the UVB-responsive system with blue and red light-inducible gene control technology enabled multi-chromatic multi-gene control and was applied to angiogenic signaling processes.
By combining this UVB-responsive system with blue and red light-inducible gene control technology, we demonstrate multi-chromatic multi-gene control by differentially expressing three genes in a single cell culture in mammalian cells, and we apply this system for the multi-chromatic control of angiogenic signaling processes.
Combining the UVB-responsive system with blue and red light-inducible gene control technology enabled multi-chromatic multi-gene control and was applied to angiogenic signaling processes.
By combining this UVB-responsive system with blue and red light-inducible gene control technology, we demonstrate multi-chromatic multi-gene control by differentially expressing three genes in a single cell culture in mammalian cells, and we apply this system for the multi-chromatic control of angiogenic signaling processes.
Combining the UVB-responsive system with blue and red light-inducible gene control technology enabled multi-chromatic multi-gene control and was applied to angiogenic signaling processes.
By combining this UVB-responsive system with blue and red light-inducible gene control technology, we demonstrate multi-chromatic multi-gene control by differentially expressing three genes in a single cell culture in mammalian cells, and we apply this system for the multi-chromatic control of angiogenic signaling processes.
Combining the UVB-responsive system with blue and red light-inducible gene control technology enabled multi-chromatic multi-gene control and was applied to angiogenic signaling processes.
By combining this UVB-responsive system with blue and red light-inducible gene control technology, we demonstrate multi-chromatic multi-gene control by differentially expressing three genes in a single cell culture in mammalian cells, and we apply this system for the multi-chromatic control of angiogenic signaling processes.
Combining the UVB-responsive system with blue and red light-inducible gene control technology enabled multi-chromatic multi-gene control and was applied to angiogenic signaling processes.
By combining this UVB-responsive system with blue and red light-inducible gene control technology, we demonstrate multi-chromatic multi-gene control by differentially expressing three genes in a single cell culture in mammalian cells, and we apply this system for the multi-chromatic control of angiogenic signaling processes.
The study demonstrates multi-chromatic expression control in mammalian cells by differentially inducing up to three genes in a single cell culture in response to different wavelengths of light.
we demonstrate for the first time multi-chromatic expression control in mammalian cells by differentially inducing up to three genes in a single cell culture in response to light of different wavelengths
genes differentially induced 3
The study demonstrates multi-chromatic expression control in mammalian cells by differentially inducing up to three genes in a single cell culture in response to different wavelengths of light.
we demonstrate for the first time multi-chromatic expression control in mammalian cells by differentially inducing up to three genes in a single cell culture in response to light of different wavelengths
genes differentially induced 3
The study demonstrates multi-chromatic expression control in mammalian cells by differentially inducing up to three genes in a single cell culture in response to different wavelengths of light.
we demonstrate for the first time multi-chromatic expression control in mammalian cells by differentially inducing up to three genes in a single cell culture in response to light of different wavelengths
genes differentially induced 3
The study demonstrates multi-chromatic expression control in mammalian cells by differentially inducing up to three genes in a single cell culture in response to different wavelengths of light.
we demonstrate for the first time multi-chromatic expression control in mammalian cells by differentially inducing up to three genes in a single cell culture in response to light of different wavelengths
genes differentially induced 3
The study demonstrates multi-chromatic expression control in mammalian cells by differentially inducing up to three genes in a single cell culture in response to different wavelengths of light.
we demonstrate for the first time multi-chromatic expression control in mammalian cells by differentially inducing up to three genes in a single cell culture in response to light of different wavelengths
genes differentially induced 3
The study demonstrates multi-chromatic expression control in mammalian cells by differentially inducing up to three genes in a single cell culture in response to different wavelengths of light.
we demonstrate for the first time multi-chromatic expression control in mammalian cells by differentially inducing up to three genes in a single cell culture in response to light of different wavelengths
genes differentially induced 3
The study demonstrates multi-chromatic expression control in mammalian cells by differentially inducing up to three genes in a single cell culture in response to different wavelengths of light.
we demonstrate for the first time multi-chromatic expression control in mammalian cells by differentially inducing up to three genes in a single cell culture in response to light of different wavelengths
genes differentially induced 3
The study demonstrates multi-chromatic expression control in mammalian cells by differentially inducing up to three genes in a single cell culture in response to different wavelengths of light.
we demonstrate for the first time multi-chromatic expression control in mammalian cells by differentially inducing up to three genes in a single cell culture in response to light of different wavelengths
genes differentially induced 3
The study demonstrates multi-chromatic expression control in mammalian cells by differentially inducing up to three genes in a single cell culture in response to different wavelengths of light.
we demonstrate for the first time multi-chromatic expression control in mammalian cells by differentially inducing up to three genes in a single cell culture in response to light of different wavelengths
genes differentially induced 3
The authors developed a UVB-inducible expression system by designing a UVB-responsive split transcription factor based on UVR8 and the WD40 domain of COP1.
we developed an ultraviolet B (UVB)-inducible expression system by designing a UVB-responsive split transcription factor based on the Arabidopsis thaliana UVB receptor UVR8 and the WD40 domain of COP1
The authors developed a UVB-inducible expression system by designing a UVB-responsive split transcription factor based on UVR8 and the WD40 domain of COP1.
we developed an ultraviolet B (UVB)-inducible expression system by designing a UVB-responsive split transcription factor based on the Arabidopsis thaliana UVB receptor UVR8 and the WD40 domain of COP1
The authors developed a UVB-inducible expression system by designing a UVB-responsive split transcription factor based on UVR8 and the WD40 domain of COP1.
we developed an ultraviolet B (UVB)-inducible expression system by designing a UVB-responsive split transcription factor based on the Arabidopsis thaliana UVB receptor UVR8 and the WD40 domain of COP1
The authors developed a UVB-inducible expression system by designing a UVB-responsive split transcription factor based on UVR8 and the WD40 domain of COP1.
we developed an ultraviolet B (UVB)-inducible expression system by designing a UVB-responsive split transcription factor based on the Arabidopsis thaliana UVB receptor UVR8 and the WD40 domain of COP1
The authors developed a UVB-inducible expression system by designing a UVB-responsive split transcription factor based on UVR8 and the WD40 domain of COP1.
we developed an ultraviolet B (UVB)-inducible expression system by designing a UVB-responsive split transcription factor based on the Arabidopsis thaliana UVB receptor UVR8 and the WD40 domain of COP1
The authors developed a UVB-inducible expression system by designing a UVB-responsive split transcription factor based on UVR8 and the WD40 domain of COP1.
we developed an ultraviolet B (UVB)-inducible expression system by designing a UVB-responsive split transcription factor based on the Arabidopsis thaliana UVB receptor UVR8 and the WD40 domain of COP1
The authors developed a UVB-inducible expression system by designing a UVB-responsive split transcription factor based on UVR8 and the WD40 domain of COP1.
we developed an ultraviolet B (UVB)-inducible expression system by designing a UVB-responsive split transcription factor based on the Arabidopsis thaliana UVB receptor UVR8 and the WD40 domain of COP1
The authors developed a UVB-inducible expression system by designing a UVB-responsive split transcription factor based on UVR8 and the WD40 domain of COP1.
we developed an ultraviolet B (UVB)-inducible expression system by designing a UVB-responsive split transcription factor based on the Arabidopsis thaliana UVB receptor UVR8 and the WD40 domain of COP1
The authors developed a UVB-inducible expression system by designing a UVB-responsive split transcription factor based on UVR8 and the WD40 domain of COP1.
we developed an ultraviolet B (UVB)-inducible expression system by designing a UVB-responsive split transcription factor based on the Arabidopsis thaliana UVB receptor UVR8 and the WD40 domain of COP1
This portfolio of optogenetic tools enables the design and implementation of synthetic biological networks with unmatched spatiotemporal precision for future research and biomedical applications.
This portfolio of optogenetic tools enables the design and implementation of synthetic biological networks showing unmatched spatiotemporal precision for future research and biomedical applications.
This portfolio of optogenetic tools enables the design and implementation of synthetic biological networks with unmatched spatiotemporal precision for future research and biomedical applications.
This portfolio of optogenetic tools enables the design and implementation of synthetic biological networks showing unmatched spatiotemporal precision for future research and biomedical applications.
This portfolio of optogenetic tools enables the design and implementation of synthetic biological networks with unmatched spatiotemporal precision for future research and biomedical applications.
This portfolio of optogenetic tools enables the design and implementation of synthetic biological networks showing unmatched spatiotemporal precision for future research and biomedical applications.
This portfolio of optogenetic tools enables the design and implementation of synthetic biological networks with unmatched spatiotemporal precision for future research and biomedical applications.
This portfolio of optogenetic tools enables the design and implementation of synthetic biological networks showing unmatched spatiotemporal precision for future research and biomedical applications.
This portfolio of optogenetic tools enables the design and implementation of synthetic biological networks with unmatched spatiotemporal precision for future research and biomedical applications.
This portfolio of optogenetic tools enables the design and implementation of synthetic biological networks showing unmatched spatiotemporal precision for future research and biomedical applications.
This portfolio of optogenetic tools enables the design and implementation of synthetic biological networks with unmatched spatiotemporal precision for future research and biomedical applications.
This portfolio of optogenetic tools enables the design and implementation of synthetic biological networks showing unmatched spatiotemporal precision for future research and biomedical applications.
This portfolio of optogenetic tools enables the design and implementation of synthetic biological networks with unmatched spatiotemporal precision for future research and biomedical applications.
This portfolio of optogenetic tools enables the design and implementation of synthetic biological networks showing unmatched spatiotemporal precision for future research and biomedical applications.
This portfolio of optogenetic tools enables the design and implementation of synthetic biological networks with unmatched spatiotemporal precision for future research and biomedical applications.
This portfolio of optogenetic tools enables the design and implementation of synthetic biological networks showing unmatched spatiotemporal precision for future research and biomedical applications.
This portfolio of optogenetic tools enables the design and implementation of synthetic biological networks with unmatched spatiotemporal precision for future research and biomedical applications.
This portfolio of optogenetic tools enables the design and implementation of synthetic biological networks showing unmatched spatiotemporal precision for future research and biomedical applications.
A quantitative model was used to determine critical system parameters.
Based on a quantitative model, we determined critical system parameters.
A quantitative model was used to determine critical system parameters.
Based on a quantitative model, we determined critical system parameters.
A quantitative model was used to determine critical system parameters.
Based on a quantitative model, we determined critical system parameters.
A quantitative model was used to determine critical system parameters.
Based on a quantitative model, we determined critical system parameters.
A quantitative model was used to determine critical system parameters.
Based on a quantitative model, we determined critical system parameters.
A quantitative model was used to determine critical system parameters.
Based on a quantitative model, we determined critical system parameters.
A quantitative model was used to determine critical system parameters.
Based on a quantitative model, we determined critical system parameters.
A quantitative model was used to determine critical system parameters.
Based on a quantitative model, we determined critical system parameters.
A quantitative model was used to determine critical system parameters.
Based on a quantitative model, we determined critical system parameters.
The UVB-inducible expression system enabled up to 800-fold UVB-induced gene expression in human, monkey, hamster, and mouse cells.
The system allowed high (up to 800-fold) UVB-induced gene expression in human, monkey, hamster and mouse cells.
UVB-induced gene expression fold induction 800 fold
The UVB-inducible expression system enabled up to 800-fold UVB-induced gene expression in human, monkey, hamster, and mouse cells.
The system allowed high (up to 800-fold) UVB-induced gene expression in human, monkey, hamster and mouse cells.
UVB-induced gene expression fold induction 800 fold
The UVB-inducible expression system enabled up to 800-fold UVB-induced gene expression in human, monkey, hamster, and mouse cells.
The system allowed high (up to 800-fold) UVB-induced gene expression in human, monkey, hamster and mouse cells.
UVB-induced gene expression fold induction 800 fold
The UVB-inducible expression system enabled up to 800-fold UVB-induced gene expression in human, monkey, hamster, and mouse cells.
The system allowed high (up to 800-fold) UVB-induced gene expression in human, monkey, hamster and mouse cells.
UVB-induced gene expression fold induction 800 fold
The UVB-inducible expression system enabled up to 800-fold UVB-induced gene expression in human, monkey, hamster, and mouse cells.
The system allowed high (up to 800-fold) UVB-induced gene expression in human, monkey, hamster and mouse cells.
UVB-induced gene expression fold induction 800 fold
The UVB-inducible expression system enabled up to 800-fold UVB-induced gene expression in human, monkey, hamster, and mouse cells.
The system allowed high (up to 800-fold) UVB-induced gene expression in human, monkey, hamster and mouse cells.
UVB-induced gene expression fold induction 800 fold
The UVB-inducible expression system enabled up to 800-fold UVB-induced gene expression in human, monkey, hamster, and mouse cells.
The system allowed high (up to 800-fold) UVB-induced gene expression in human, monkey, hamster and mouse cells.
UVB-induced gene expression fold induction 800 fold
The UVB-inducible expression system enabled up to 800-fold UVB-induced gene expression in human, monkey, hamster, and mouse cells.
The system allowed high (up to 800-fold) UVB-induced gene expression in human, monkey, hamster and mouse cells.
UVB-induced gene expression fold induction 800 fold
The UVB-inducible expression system enabled up to 800-fold UVB-induced gene expression in human, monkey, hamster, and mouse cells.
The system allowed high (up to 800-fold) UVB-induced gene expression in human, monkey, hamster and mouse cells.
UVB-induced gene expression fold induction 800 fold
Approval Evidence
1 source4 linked approval claimsfirst-pass slug uvb-inducible-expression-system
we developed an ultraviolet B (UVB)-inducible expression system by designing a UVB-responsive split transcription factor based on the Arabidopsis thaliana UVB receptor UVR8 and the WD40 domain of COP1
Source:
applicationsupports
Combining the UVB-responsive system with blue and red light-inducible gene control technology enabled multi-chromatic multi-gene control and was applied to angiogenic signaling processes.
By combining this UVB-responsive system with blue and red light-inducible gene control technology, we demonstrate multi-chromatic multi-gene control by differentially expressing three genes in a single cell culture in mammalian cells, and we apply this system for the multi-chromatic control of angiogenic signaling processes.
Source:
engineering resultsupports
The authors developed a UVB-inducible expression system by designing a UVB-responsive split transcription factor based on UVR8 and the WD40 domain of COP1.
we developed an ultraviolet B (UVB)-inducible expression system by designing a UVB-responsive split transcription factor based on the Arabidopsis thaliana UVB receptor UVR8 and the WD40 domain of COP1
Source:
modeling resultsupports
A quantitative model was used to determine critical system parameters.
Based on a quantitative model, we determined critical system parameters.
Source:
performancesupports
The UVB-inducible expression system enabled up to 800-fold UVB-induced gene expression in human, monkey, hamster, and mouse cells.
The system allowed high (up to 800-fold) UVB-induced gene expression in human, monkey, hamster and mouse cells.
Source:
Comparisons
Source-backed strengths
The reported system enabled UVB-triggered transcriptional activation in mammalian cells through a split transcription factor design. In combination with blue and red light-inducible technologies, it supported multichromatic control of up to three genes in a single cell culture and was applied to angiogenic signaling.
Source:
high inducibility up to 800-fold
Source:
reported across human, monkey, hamster and mouse cells
Ranked Citations
- 1.