Source 1primary paper1995PubMed
Toolkit/UVB irradiation
UVB irradiation
Also known as: UVB light
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
UVB irradiation is a light-based cellular perturbation method reported to inhibit proliferation of human melanocytes by inducing G1 cell-cycle arrest. In the cited 1995 study, this response was associated with prolonged expression of p53, p21Waf-1/SDI-1/Cip-1, and c-fos and with prolonged inhibition of retinoblastoma protein phosphorylation.
Usefulness & Problems
Why this is useful
This method is useful for experimentally imposing a defined cell-cycle arrest response in human melanocytes using light exposure. It provides a way to study UVB-responsive signaling and cell-cycle control pathways linked to p53, p21Waf-1/SDI-1/Cip-1, c-fos, and retinoblastoma protein phosphorylation.
Problem solved
UVB irradiation addresses the need for a perturbation that suppresses human melanocyte proliferation and drives G1 arrest while engaging endogenous stress-response and cell-cycle regulatory pathways. The cited evidence specifically links this perturbation to sustained p53 and p21Waf-1/SDI-1/Cip-1 expression and inhibition of retinoblastoma protein phosphorylation.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete method used to build, optimize, or evolve an engineered system.
Mechanisms
inhibition of retinoblastoma protein phosphorylationlight-induced cell-cycle arrestprolonged c-fos expressionprolonged expression of p21waf-1/sdi-1/cip-1prolonged expression of p53transcriptional induction of p53 and p21waf-1/sdi-1/cip-1transcriptional induction or prolonged expression of p21waf-1/sdi-1/cip-1transcriptional induction or prolonged expression of p53Techniques
No technique tags yet.
Target processes
signalingInput: Light
Implementation Constraints
Implementation requires UVB light exposure of human melanocytes, but the supplied evidence does not report the irradiation parameters or apparatus. The measured outputs in the cited study included proliferation, G1 arrest, p53 and p21Waf-1/SDI-1/Cip-1 expression, c-fos expression, and retinoblastoma protein phosphorylation status.
The evidence provided comes from a single cited study in human melanocytes, so validation breadth is limited. The supplied evidence does not specify irradiation dose, wavelength range within UVB, delivery setup, reversibility, or performance in other cell types or organisms.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
UVB irradiation inhibits human melanocyte proliferation by causing G1 arrest.
UVB irradiation inhibits melanocyte proliferation by causing arrest in G1
UVB irradiation inhibits human melanocyte proliferation by causing G1 arrest.
UVB irradiation inhibits melanocyte proliferation by causing arrest in G1
UVB irradiation inhibits human melanocyte proliferation by causing G1 arrest.
UVB irradiation inhibits melanocyte proliferation by causing arrest in G1
UVB irradiation inhibits human melanocyte proliferation by causing G1 arrest.
UVB irradiation inhibits melanocyte proliferation by causing arrest in G1
UVB irradiation inhibits human melanocyte proliferation by causing G1 arrest.
UVB irradiation inhibits melanocyte proliferation by causing arrest in G1
UVB irradiation inhibits human melanocyte proliferation by causing G1 arrest.
UVB irradiation inhibits melanocyte proliferation by causing arrest in G1
UVB irradiation inhibits human melanocyte proliferation by causing G1 arrest.
UVB irradiation inhibits melanocyte proliferation by causing arrest in G1
After UV irradiation, p53 and p21Waf-1/SDI-1/Cip-1 were expressed at high levels for at least 2 days in human melanocytes.
The tumor suppressor p53 and the p53-inducible cyclin-dependent kinase inhibitor protein p21Waf-1/SDI-1/Cip-1 were expressed at high levels for at least 2 days after UV-irradiation
expression duration 2 days
After UV irradiation, p53 and p21Waf-1/SDI-1/Cip-1 were expressed at high levels for at least 2 days in human melanocytes.
The tumor suppressor p53 and the p53-inducible cyclin-dependent kinase inhibitor protein p21Waf-1/SDI-1/Cip-1 were expressed at high levels for at least 2 days after UV-irradiation
expression duration 2 days
After UV irradiation, p53 and p21Waf-1/SDI-1/Cip-1 were expressed at high levels for at least 2 days in human melanocytes.
The tumor suppressor p53 and the p53-inducible cyclin-dependent kinase inhibitor protein p21Waf-1/SDI-1/Cip-1 were expressed at high levels for at least 2 days after UV-irradiation
expression duration 2 days
After UV irradiation, p53 and p21Waf-1/SDI-1/Cip-1 were expressed at high levels for at least 2 days in human melanocytes.
The tumor suppressor p53 and the p53-inducible cyclin-dependent kinase inhibitor protein p21Waf-1/SDI-1/Cip-1 were expressed at high levels for at least 2 days after UV-irradiation
expression duration 2 days
After UV irradiation, p53 and p21Waf-1/SDI-1/Cip-1 were expressed at high levels for at least 2 days in human melanocytes.
The tumor suppressor p53 and the p53-inducible cyclin-dependent kinase inhibitor protein p21Waf-1/SDI-1/Cip-1 were expressed at high levels for at least 2 days after UV-irradiation
expression duration 2 days
After UV irradiation, p53 and p21Waf-1/SDI-1/Cip-1 were expressed at high levels for at least 2 days in human melanocytes.
The tumor suppressor p53 and the p53-inducible cyclin-dependent kinase inhibitor protein p21Waf-1/SDI-1/Cip-1 were expressed at high levels for at least 2 days after UV-irradiation
expression duration 2 days
After UV irradiation, p53 and p21Waf-1/SDI-1/Cip-1 were expressed at high levels for at least 2 days in human melanocytes.
The tumor suppressor p53 and the p53-inducible cyclin-dependent kinase inhibitor protein p21Waf-1/SDI-1/Cip-1 were expressed at high levels for at least 2 days after UV-irradiation
expression duration 2 days
UVB light induced prolonged c-fos expression in human melanocytes.
UVB light induced a prolonged c-fos expression
UVB light induced prolonged c-fos expression in human melanocytes.
UVB light induced a prolonged c-fos expression
UVB light induced prolonged c-fos expression in human melanocytes.
UVB light induced a prolonged c-fos expression
UVB light induced prolonged c-fos expression in human melanocytes.
UVB light induced a prolonged c-fos expression
UVB light induced prolonged c-fos expression in human melanocytes.
UVB light induced a prolonged c-fos expression
UVB light induced prolonged c-fos expression in human melanocytes.
UVB light induced a prolonged c-fos expression
UVB light induced prolonged c-fos expression in human melanocytes.
UVB light induced a prolonged c-fos expression
Phosphorylation of retinoblastoma protein was halted in UVB-irradiated human melanocytes and correlated with p21Waf-1/SDI-1/Cip-1 expression.
phosphorylation of Rb, the retinoblastoma tumor suppressor gene product, was halted in UVB-irradiated cells and correlated with the expression of the protein p21Waf-1/SDI-1/Cip-1
Phosphorylation of retinoblastoma protein was halted in UVB-irradiated human melanocytes and correlated with p21Waf-1/SDI-1/Cip-1 expression.
phosphorylation of Rb, the retinoblastoma tumor suppressor gene product, was halted in UVB-irradiated cells and correlated with the expression of the protein p21Waf-1/SDI-1/Cip-1
Phosphorylation of retinoblastoma protein was halted in UVB-irradiated human melanocytes and correlated with p21Waf-1/SDI-1/Cip-1 expression.
phosphorylation of Rb, the retinoblastoma tumor suppressor gene product, was halted in UVB-irradiated cells and correlated with the expression of the protein p21Waf-1/SDI-1/Cip-1
Phosphorylation of retinoblastoma protein was halted in UVB-irradiated human melanocytes and correlated with p21Waf-1/SDI-1/Cip-1 expression.
phosphorylation of Rb, the retinoblastoma tumor suppressor gene product, was halted in UVB-irradiated cells and correlated with the expression of the protein p21Waf-1/SDI-1/Cip-1
Phosphorylation of retinoblastoma protein was halted in UVB-irradiated human melanocytes and correlated with p21Waf-1/SDI-1/Cip-1 expression.
phosphorylation of Rb, the retinoblastoma tumor suppressor gene product, was halted in UVB-irradiated cells and correlated with the expression of the protein p21Waf-1/SDI-1/Cip-1
Phosphorylation of retinoblastoma protein was halted in UVB-irradiated human melanocytes and correlated with p21Waf-1/SDI-1/Cip-1 expression.
phosphorylation of Rb, the retinoblastoma tumor suppressor gene product, was halted in UVB-irradiated cells and correlated with the expression of the protein p21Waf-1/SDI-1/Cip-1
Phosphorylation of retinoblastoma protein was halted in UVB-irradiated human melanocytes and correlated with p21Waf-1/SDI-1/Cip-1 expression.
phosphorylation of Rb, the retinoblastoma tumor suppressor gene product, was halted in UVB-irradiated cells and correlated with the expression of the protein p21Waf-1/SDI-1/Cip-1
UVB irradiation did not stimulate the analyzed tyrosine kinases, Baf-1, and ERK2 in human melanocytes, in contrast to the UVC response.
in contrast to the UVC response, exposure to UVB irradiation did not stimulate the above kinases
UVB irradiation did not stimulate the analyzed tyrosine kinases, Baf-1, and ERK2 in human melanocytes, in contrast to the UVC response.
in contrast to the UVC response, exposure to UVB irradiation did not stimulate the above kinases
UVB irradiation did not stimulate the analyzed tyrosine kinases, Baf-1, and ERK2 in human melanocytes, in contrast to the UVC response.
in contrast to the UVC response, exposure to UVB irradiation did not stimulate the above kinases
UVB irradiation did not stimulate the analyzed tyrosine kinases, Baf-1, and ERK2 in human melanocytes, in contrast to the UVC response.
in contrast to the UVC response, exposure to UVB irradiation did not stimulate the above kinases
UVB irradiation did not stimulate the analyzed tyrosine kinases, Baf-1, and ERK2 in human melanocytes, in contrast to the UVC response.
in contrast to the UVC response, exposure to UVB irradiation did not stimulate the above kinases
UVB irradiation did not stimulate the analyzed tyrosine kinases, Baf-1, and ERK2 in human melanocytes, in contrast to the UVC response.
in contrast to the UVC response, exposure to UVB irradiation did not stimulate the above kinases
UVB irradiation did not stimulate the analyzed tyrosine kinases, Baf-1, and ERK2 in human melanocytes, in contrast to the UVC response.
in contrast to the UVC response, exposure to UVB irradiation did not stimulate the above kinases
Approval Evidence
1 source5 linked approval claimsfirst-pass slug uvb-irradiation
UVB irradiation inhibits melanocyte proliferation by causing arrest in G1
Source:
cell cycle effectsupports
UVB irradiation inhibits human melanocyte proliferation by causing G1 arrest.
UVB irradiation inhibits melanocyte proliferation by causing arrest in G1
Source:
expression changesupports
After UV irradiation, p53 and p21Waf-1/SDI-1/Cip-1 were expressed at high levels for at least 2 days in human melanocytes.
The tumor suppressor p53 and the p53-inducible cyclin-dependent kinase inhibitor protein p21Waf-1/SDI-1/Cip-1 were expressed at high levels for at least 2 days after UV-irradiation
Source:
expression changesupports
UVB light induced prolonged c-fos expression in human melanocytes.
UVB light induced a prolonged c-fos expression
Source:
phosphorylation changesupports
Phosphorylation of retinoblastoma protein was halted in UVB-irradiated human melanocytes and correlated with p21Waf-1/SDI-1/Cip-1 expression.
phosphorylation of Rb, the retinoblastoma tumor suppressor gene product, was halted in UVB-irradiated cells and correlated with the expression of the protein p21Waf-1/SDI-1/Cip-1
Source:
signaling responsesupports
UVB irradiation did not stimulate the analyzed tyrosine kinases, Baf-1, and ERK2 in human melanocytes, in contrast to the UVC response.
in contrast to the UVC response, exposure to UVB irradiation did not stimulate the above kinases
Source:
Comparisons
Source-backed strengths
The reported phenotype is mechanistically associated with multiple molecular readouts, including G1 arrest, prolonged p53 and p21Waf-1/SDI-1/Cip-1 expression for at least 2 days after UV irradiation, prolonged c-fos expression, and inhibition of retinoblastoma protein phosphorylation. These linked observations provide a coherent cellular response profile in human melanocytes.
Ranked Citations
- 1.