Source 1review2014Current Protocols in Cell Biology
Toolkit/UVR8/UVR8
UVR8/UVR8
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
UVR8/UVR8 is a light-controlled protein interaction system described as one of two optical dimerizer platforms in a protocol-focused study. It is presented for regulating cellular processes including transcription, protein localization, and protein secretion using light.
Usefulness & Problems
Why this is useful
The system is useful as an optical dimerizer for perturbing protein interactions with light in cell biological experiments. The cited protocol positions it as a platform for controlling transcription, protein localization, and protein secretion, and emphasizes integration with photoexcitation hardware and imaging reporters.
Source:
We provide detailed protocols for experiments using two dimerization systems we previously developed, CRY2/CIB and UVR8/UVR8, for use in controlling transcription, protein localization, and protein secretion using light.
Source:
we provide instructions and software for constructing a pulse-controlled LED device for use in experiments requiring extended light treatments
Problem solved
UVR8/UVR8 helps address the need for light-dependent control of intracellular protein interactions in order to manipulate cellular processes with temporal experimental input. The available evidence specifically frames this around designing optical dimerizer experiments for transcriptional control, localization control, and secretion-related assays.
Source:
We provide detailed protocols for experiments using two dimerization systems we previously developed, CRY2/CIB and UVR8/UVR8, for use in controlling transcription, protein localization, and protein secretion using light.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
HeterodimerizationTechniques
No technique tags yet.
Target processes
localizationrecombinationtranscriptionInput: Light
Implementation Constraints
The protocol indicates that experiment design requires selecting a dimerizer system, photoexcitation sources, and coordinated imaging reporters. Beyond these general considerations, the provided evidence does not specify construct design, cofactors, expression systems, or delivery methods for UVR8/UVR8.
The supplied evidence is limited to a protocol-oriented description and does not provide quantitative performance data, molecular architecture, wavelength specifications, kinetics, reversibility, or comparative benchmarking for UVR8/UVR8. Independent replication and validation in contexts beyond the cited protocol are not documented in the provided evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
CRY2/CIB and UVR8/UVR8 are presented for controlling transcription, protein localization, and protein secretion using light.
We provide detailed protocols for experiments using two dimerization systems we previously developed, CRY2/CIB and UVR8/UVR8, for use in controlling transcription, protein localization, and protein secretion using light.
CRY2/CIB and UVR8/UVR8 are presented for controlling transcription, protein localization, and protein secretion using light.
We provide detailed protocols for experiments using two dimerization systems we previously developed, CRY2/CIB and UVR8/UVR8, for use in controlling transcription, protein localization, and protein secretion using light.
CRY2/CIB and UVR8/UVR8 are presented for controlling transcription, protein localization, and protein secretion using light.
We provide detailed protocols for experiments using two dimerization systems we previously developed, CRY2/CIB and UVR8/UVR8, for use in controlling transcription, protein localization, and protein secretion using light.
CRY2/CIB and UVR8/UVR8 are presented for controlling transcription, protein localization, and protein secretion using light.
We provide detailed protocols for experiments using two dimerization systems we previously developed, CRY2/CIB and UVR8/UVR8, for use in controlling transcription, protein localization, and protein secretion using light.
CRY2/CIB and UVR8/UVR8 are presented for controlling transcription, protein localization, and protein secretion using light.
We provide detailed protocols for experiments using two dimerization systems we previously developed, CRY2/CIB and UVR8/UVR8, for use in controlling transcription, protein localization, and protein secretion using light.
CRY2/CIB and UVR8/UVR8 are presented for controlling transcription, protein localization, and protein secretion using light.
We provide detailed protocols for experiments using two dimerization systems we previously developed, CRY2/CIB and UVR8/UVR8, for use in controlling transcription, protein localization, and protein secretion using light.
CRY2/CIB and UVR8/UVR8 are presented for controlling transcription, protein localization, and protein secretion using light.
We provide detailed protocols for experiments using two dimerization systems we previously developed, CRY2/CIB and UVR8/UVR8, for use in controlling transcription, protein localization, and protein secretion using light.
Design of optical dimerizer experiments includes choosing a dimerizer system, photoexcitation sources, and coordinated imaging reporters.
Here we discuss the design of optical dimerizer experiments, including choice of a dimerizer system, photoexcitation sources, and the coordinate use of imaging reporters.
Design of optical dimerizer experiments includes choosing a dimerizer system, photoexcitation sources, and coordinated imaging reporters.
Here we discuss the design of optical dimerizer experiments, including choice of a dimerizer system, photoexcitation sources, and the coordinate use of imaging reporters.
Design of optical dimerizer experiments includes choosing a dimerizer system, photoexcitation sources, and coordinated imaging reporters.
Here we discuss the design of optical dimerizer experiments, including choice of a dimerizer system, photoexcitation sources, and the coordinate use of imaging reporters.
Design of optical dimerizer experiments includes choosing a dimerizer system, photoexcitation sources, and coordinated imaging reporters.
Here we discuss the design of optical dimerizer experiments, including choice of a dimerizer system, photoexcitation sources, and the coordinate use of imaging reporters.
Design of optical dimerizer experiments includes choosing a dimerizer system, photoexcitation sources, and coordinated imaging reporters.
Here we discuss the design of optical dimerizer experiments, including choice of a dimerizer system, photoexcitation sources, and the coordinate use of imaging reporters.
Design of optical dimerizer experiments includes choosing a dimerizer system, photoexcitation sources, and coordinated imaging reporters.
Here we discuss the design of optical dimerizer experiments, including choice of a dimerizer system, photoexcitation sources, and the coordinate use of imaging reporters.
Design of optical dimerizer experiments includes choosing a dimerizer system, photoexcitation sources, and coordinated imaging reporters.
Here we discuss the design of optical dimerizer experiments, including choice of a dimerizer system, photoexcitation sources, and the coordinate use of imaging reporters.
Numerous new and versatile optical dimerizer systems have been developed in recent years.
In recent years, numerous new and versatile dimerizer systems have been developed.
Numerous new and versatile optical dimerizer systems have been developed in recent years.
In recent years, numerous new and versatile dimerizer systems have been developed.
Numerous new and versatile optical dimerizer systems have been developed in recent years.
In recent years, numerous new and versatile dimerizer systems have been developed.
Numerous new and versatile optical dimerizer systems have been developed in recent years.
In recent years, numerous new and versatile dimerizer systems have been developed.
Numerous new and versatile optical dimerizer systems have been developed in recent years.
In recent years, numerous new and versatile dimerizer systems have been developed.
Numerous new and versatile optical dimerizer systems have been developed in recent years.
In recent years, numerous new and versatile dimerizer systems have been developed.
Numerous new and versatile optical dimerizer systems have been developed in recent years.
In recent years, numerous new and versatile dimerizer systems have been developed.
Optical dimerizers are genetically encoded actuators that enable light control of protein-protein interactions.
Genetically encoded actuators that allow control of protein-protein interactions using light, termed 'optical dimerizers'
Optical dimerizers are genetically encoded actuators that enable light control of protein-protein interactions.
Genetically encoded actuators that allow control of protein-protein interactions using light, termed 'optical dimerizers'
Optical dimerizers are genetically encoded actuators that enable light control of protein-protein interactions.
Genetically encoded actuators that allow control of protein-protein interactions using light, termed 'optical dimerizers'
Optical dimerizers are genetically encoded actuators that enable light control of protein-protein interactions.
Genetically encoded actuators that allow control of protein-protein interactions using light, termed 'optical dimerizers'
Optical dimerizers are genetically encoded actuators that enable light control of protein-protein interactions.
Genetically encoded actuators that allow control of protein-protein interactions using light, termed 'optical dimerizers'
Optical dimerizers are genetically encoded actuators that enable light control of protein-protein interactions.
Genetically encoded actuators that allow control of protein-protein interactions using light, termed 'optical dimerizers'
Optical dimerizers are genetically encoded actuators that enable light control of protein-protein interactions.
Genetically encoded actuators that allow control of protein-protein interactions using light, termed 'optical dimerizers'
A pulse-controlled LED device is provided for experiments requiring extended light treatments.
we provide instructions and software for constructing a pulse-controlled LED device for use in experiments requiring extended light treatments
A pulse-controlled LED device is provided for experiments requiring extended light treatments.
we provide instructions and software for constructing a pulse-controlled LED device for use in experiments requiring extended light treatments
A pulse-controlled LED device is provided for experiments requiring extended light treatments.
we provide instructions and software for constructing a pulse-controlled LED device for use in experiments requiring extended light treatments
A pulse-controlled LED device is provided for experiments requiring extended light treatments.
we provide instructions and software for constructing a pulse-controlled LED device for use in experiments requiring extended light treatments
A pulse-controlled LED device is provided for experiments requiring extended light treatments.
we provide instructions and software for constructing a pulse-controlled LED device for use in experiments requiring extended light treatments
A pulse-controlled LED device is provided for experiments requiring extended light treatments.
we provide instructions and software for constructing a pulse-controlled LED device for use in experiments requiring extended light treatments
A pulse-controlled LED device is provided for experiments requiring extended light treatments.
we provide instructions and software for constructing a pulse-controlled LED device for use in experiments requiring extended light treatments
Approval Evidence
1 source1 linked approval claimfirst-pass slug uvr8-uvr8
We provide detailed protocols for experiments using two dimerization systems we previously developed, CRY2/CIB and UVR8/UVR8
Source:
application scopesupports
CRY2/CIB and UVR8/UVR8 are presented for controlling transcription, protein localization, and protein secretion using light.
We provide detailed protocols for experiments using two dimerization systems we previously developed, CRY2/CIB and UVR8/UVR8, for use in controlling transcription, protein localization, and protein secretion using light.
Source:
Comparisons
Source-backed strengths
A protocol resource is available that provides detailed experimental procedures for using UVR8/UVR8. The study presents UVR8/UVR8 as an established dimerization system alongside CRY2/CIB for multiple application classes, indicating practical utility across several light-controlled cell biology use cases.
Ranked Citations
- 1.