Source 1primary paper2019MED
Toolkit/vertically deployed surface acoustic wave platform
vertically deployed surface acoustic wave platform
Also known as: VD-SAW, VD-SAW platform
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
In this work, we developed a vertically deployed surface acoustic wave (VD-SAW) platform that generates 30 MHz focused ultrasound wave for mechanical stimulation of single cells.
Usefulness & Problems
Why this is useful
The VD-SAW platform generates 30 MHz focused ultrasound for mechanical stimulation of single cells. The paper uses it to probe ultrasound-induced mechanotransduction in HEK293T cells.; mechanical stimulation of single cells; investigating ultrasound-induced mechanotransduction at the single cell level
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The VD-SAW platform generates 30 MHz focused ultrasound for mechanical stimulation of single cells. The paper uses it to probe ultrasound-induced mechanotransduction in HEK293T cells.
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mechanical stimulation of single cells
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investigating ultrasound-induced mechanotransduction at the single cell level
Problem solved
It provides a way to deliver localized ultrasound stimulation for studying mechanosensitive responses at the single-cell level.; provides a focused ultrasound platform for localized single-cell mechanical stimulation
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It provides a way to deliver localized ultrasound stimulation for studying mechanosensitive responses at the single-cell level.
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provides a focused ultrasound platform for localized single-cell mechanical stimulation
Problem links
provides a focused ultrasound platform for localized single-cell mechanical stimulation
LiteratureIt provides a way to deliver localized ultrasound stimulation for studying mechanosensitive responses at the single-cell level.
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It provides a way to deliver localized ultrasound stimulation for studying mechanosensitive responses at the single-cell level.
Published Workflows
Objective: Investigate the role of Piezo1 in mediating intracellular calcium responses of HEK293T cells to pulsed ultrasound using a single-cell VD-SAW stimulation platform.
Why it works: The study compares responses across Piezo1-transfected and Piezo1-knockout HEK293T cells while varying ultrasound pulse parameters, allowing the authors to relate calcium responses to both Piezo1 presence and stimulation regime.
Piezo1-mediated mechanotransductionfocused ultrasound stimulationsingle-cell calcium-response measurementgenotype/construct comparison
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Techniques
Functional AssayTarget processes
recombinationImplementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
The study uses the platform with pulsed ultrasound settings including 1.6 MPa peak pressure, 20% duty cycle, and 60 s total treatment time. It is applied in single-cell calcium-response experiments in HEK293T cells.; used with pulsed ultrasound at 1.6 MPa peak pressure, 20% duty cycle, and 60 s total treatment time in this study
Independent follow-up evidence is still limited. Validation breadth across biological contexts is still narrow. Independent reuse still looks limited, so the evidence base may be fragile. No canonical validation observations are stored yet, so context-specific performance remains under-specified.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Compared with 200 Hz PRF and 1 ms burst duration, 2 Hz PRF and 100 ms burst duration produces higher calcium response probability, stronger and faster calcium increase, and greater cell displacement.
burst duration 100 msburst duration 1 mspulse repetition frequency 2 Hzpulse repetition frequency 200 Hz
Ultrasound-elicited calcium response depends critically on pulse repetition frequency or burst duration.
duty cycle 20 %peak pressure 1.6 MPatotal treatment time 60 s
The VD-SAW platform generates 30 MHz focused ultrasound for mechanical stimulation of single cells.
ultrasound frequency 30 MHz
The VD-SAW platform is a tool for investigating ultrasound-induced mechanotransduction at the single-cell level.
Approval Evidence
1 source4 linked approval claimsfirst-pass slug vertically-deployed-surface-acoustic-wave-platform
In this work, we developed a vertically deployed surface acoustic wave (VD-SAW) platform that generates 30 MHz focused ultrasound wave for mechanical stimulation of single cells.
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parameter comparisonsupports
Compared with 200 Hz PRF and 1 ms burst duration, 2 Hz PRF and 100 ms burst duration produces higher calcium response probability, stronger and faster calcium increase, and greater cell displacement.
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stimulation parameter dependencesupports
Ultrasound-elicited calcium response depends critically on pulse repetition frequency or burst duration.
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tool capabilitysupports
The VD-SAW platform generates 30 MHz focused ultrasound for mechanical stimulation of single cells.
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use casesupports
The VD-SAW platform is a tool for investigating ultrasound-induced mechanotransduction at the single-cell level.
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Comparisons
Source-stated alternatives
The abstract refers broadly to ultrasound as a neuromodulation modality but does not name a direct alternative device platform within the paper abstract.
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The abstract refers broadly to ultrasound as a neuromodulation modality but does not name a direct alternative device platform within the paper abstract.
Source-backed strengths
described as unique and versatile for single-cell mechanotransduction studies; generates 30 MHz focused ultrasound
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described as unique and versatile for single-cell mechanotransduction studies
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generates 30 MHz focused ultrasound
Compared with ultrasonography
The abstract refers broadly to ultrasound as a neuromodulation modality but does not name a direct alternative device platform within the paper abstract.
Shared frame: source-stated alternative in extracted literature
Strengths here: described as unique and versatile for single-cell mechanotransduction studies; generates 30 MHz focused ultrasound.
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The abstract refers broadly to ultrasound as a neuromodulation modality but does not name a direct alternative device platform within the paper abstract.
Ranked Citations
- 1.