Source 1primary paper2022
Toolkit/yeast gene deletion library
yeast gene deletion library
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The yeast gene deletion library is a genome-scale collection of yeast deletion strains used to perform systems-level analysis of branched-chain alcohol toxicity. In the cited study context, it enabled identification of principles underlying sensitivity and tolerance in yeast.
Usefulness & Problems
Why this is useful
This library is useful for linking loss-of-function genotypes to cellular phenotypes at genome scale in yeast. In the cited application, it supported systematic interrogation of branched-chain alcohol toxicity and generated insights that informed design of more tolerant production strains.
Problem solved
It helps solve the problem of identifying which yeast genes and cellular functions influence sensitivity or tolerance to branched-chain alcohols. The cited work specifically used it to enable the first systems-level study of branched-chain alcohol toxicity in yeast.
Problem links
Need conditional recombination or state switching
DerivedThe yeast gene deletion library is a genome-scale collection of yeast deletion strains used to perform systems-level analysis of branched-chain alcohol toxicity. In the cited study context, it enabled identification of principles underlying sensitivity and tolerance in yeast.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Mechanisms
gene deletionTechniques
Computational DesignTarget processes
recombinationImplementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
The evidence supports that this tool consists of yeast deletion strains and was applied in yeast for toxicity phenotyping. No further practical details on construction method, selection markers, cultivation conditions, or screening workflow are provided in the supplied evidence.
The supplied evidence does not provide details on library composition, strain background, assay format, or quantitative performance. Independent replication, condition dependence, and applicability beyond the cited branched-chain alcohol study are not established by the provided evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Insights from the toxicity study enabled design of highly tolerant yeast strains with increased branched-chain alcohol production.
allowing us to design highly tolerant strains with increased BCA production
Insights from the toxicity study enabled design of highly tolerant yeast strains with increased branched-chain alcohol production.
allowing us to design highly tolerant strains with increased BCA production
Insights from the toxicity study enabled design of highly tolerant yeast strains with increased branched-chain alcohol production.
allowing us to design highly tolerant strains with increased BCA production
Insights from the toxicity study enabled design of highly tolerant yeast strains with increased branched-chain alcohol production.
allowing us to design highly tolerant strains with increased BCA production
Insights from the toxicity study enabled design of highly tolerant yeast strains with increased branched-chain alcohol production.
allowing us to design highly tolerant strains with increased BCA production
Insights from the toxicity study enabled design of highly tolerant yeast strains with increased branched-chain alcohol production.
allowing us to design highly tolerant strains with increased BCA production
Insights from the toxicity study enabled design of highly tolerant yeast strains with increased branched-chain alcohol production.
allowing us to design highly tolerant strains with increased BCA production
The yeast gene deletion library was used for a systems-level study of branched-chain alcohol toxicity in yeast that uncovered principles of sensitivity and tolerance.
The yeast gene deletion library was also utilized to conduct the first systems-level study on BCA toxicity in yeast, which uncovered key fundamental principles of yeast sensitivity and tolerance to these alcohols
The yeast gene deletion library was used for a systems-level study of branched-chain alcohol toxicity in yeast that uncovered principles of sensitivity and tolerance.
The yeast gene deletion library was also utilized to conduct the first systems-level study on BCA toxicity in yeast, which uncovered key fundamental principles of yeast sensitivity and tolerance to these alcohols
The yeast gene deletion library was used for a systems-level study of branched-chain alcohol toxicity in yeast that uncovered principles of sensitivity and tolerance.
The yeast gene deletion library was also utilized to conduct the first systems-level study on BCA toxicity in yeast, which uncovered key fundamental principles of yeast sensitivity and tolerance to these alcohols
The yeast gene deletion library was used for a systems-level study of branched-chain alcohol toxicity in yeast that uncovered principles of sensitivity and tolerance.
The yeast gene deletion library was also utilized to conduct the first systems-level study on BCA toxicity in yeast, which uncovered key fundamental principles of yeast sensitivity and tolerance to these alcohols
The yeast gene deletion library was used for a systems-level study of branched-chain alcohol toxicity in yeast that uncovered principles of sensitivity and tolerance.
The yeast gene deletion library was also utilized to conduct the first systems-level study on BCA toxicity in yeast, which uncovered key fundamental principles of yeast sensitivity and tolerance to these alcohols
The yeast gene deletion library was used for a systems-level study of branched-chain alcohol toxicity in yeast that uncovered principles of sensitivity and tolerance.
The yeast gene deletion library was also utilized to conduct the first systems-level study on BCA toxicity in yeast, which uncovered key fundamental principles of yeast sensitivity and tolerance to these alcohols
The yeast gene deletion library was used for a systems-level study of branched-chain alcohol toxicity in yeast that uncovered principles of sensitivity and tolerance.
The yeast gene deletion library was also utilized to conduct the first systems-level study on BCA toxicity in yeast, which uncovered key fundamental principles of yeast sensitivity and tolerance to these alcohols
Approval Evidence
1 source2 linked approval claimsfirst-pass slug yeast-gene-deletion-library
The yeast gene deletion library was also utilized to conduct the first systems-level study on BCA toxicity in yeast
Source:
engineering outcomesupports
Insights from the toxicity study enabled design of highly tolerant yeast strains with increased branched-chain alcohol production.
allowing us to design highly tolerant strains with increased BCA production
Source:
systems studysupports
The yeast gene deletion library was used for a systems-level study of branched-chain alcohol toxicity in yeast that uncovered principles of sensitivity and tolerance.
The yeast gene deletion library was also utilized to conduct the first systems-level study on BCA toxicity in yeast, which uncovered key fundamental principles of yeast sensitivity and tolerance to these alcohols
Source:
Comparisons
Source-backed strengths
A key strength is its genome-scale deletion format, which supports systems-level analysis rather than single-gene testing. In the cited context, findings from this analysis were sufficiently informative to guide engineering of highly tolerant yeast strains with increased branched-chain alcohol production.
Source:
allowing us to design highly tolerant strains with increased BCA production
yeast gene deletion library and cell-specific receptor subtype gene deletion mouse models address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination
Strengths here: looks easier to implement in practice.
yeast gene deletion library and CheRiff + jRCaMP1b + RH237 cardiac all-optical electrophysiology platform address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination
Strengths here: looks easier to implement in practice.
Compared with eNpHR
yeast gene deletion library and eNpHR address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination
Strengths here: looks easier to implement in practice; may avoid an exogenous cofactor requirement.
Ranked Citations
- 1.