Source 1primary paper2015ORCA Online Research @Cardiff (Cardiff University)
Toolkit/YtvA from Bacillus subtilis
YtvA from Bacillus subtilis
Taxonomy: Mechanism Branch / Component. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
YtvA from Bacillus subtilis is a LOV-domain photoswitch reported in this evidence set as a homodimeric light-responsive protein domain. It was highlighted in an optogenetics context because its relaxation half-life is longer than that of Avena sativa LOV2, suggesting potential utility as a light-controlled module.
Usefulness & Problems
Why this is useful
The cited utility is its potential suitability as an optogenetic module due to a longer relaxation half-life than Avena sativa LOV2. This property may be advantageous where a longer-lived light-activated state is desired, although the supplied evidence does not provide application-specific performance data.
Problem solved
This tool is presented as addressing the need for genetically encoded photoswitches with slower dark-state recovery than Avena sativa LOV2. In the cited context, this was framed as improving suitability for optogenetic control, but no specific downstream effector system is described in the supplied evidence.
Problem links
Need precise spatiotemporal control with light input
DerivedYtvA from Bacillus subtilis is a LOV-domain photoswitch reported as a homodimeric light-responsive protein domain. In the cited optogenetics context, it was highlighted for a longer relaxation half-life than Avena sativa LOV2, suggesting potential utility as a light-controlled module.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Component: A low-level protein part used inside a larger architecture that realizes a mechanism.
Techniques
No technique tags yet.
Target processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: multi component delivery burdenimplementation constraint: spectral hardware requirementoperating role: actuatorswitch architecture: multi componentswitch architecture: recruitment
The supplied evidence identifies YtvA as a LOV-domain protein from Bacillus subtilis and mentions an I113D mutant evaluated for monomeric behavior. No construct architecture, cofactor requirements, expression system, illumination parameters, or delivery strategy are described in the provided material.
The evidence base here is narrow and largely comparative, with no detailed quantitative kinetics, wavelength information, or functional assay results provided. For the I113D variant, monomeric behavior is only described as inferred from comparison against standards, and the abstract notes that further experiments are needed to verify this.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
YtvA is a homodimeric photoswitch with a longer relaxation half-life than Avena sativa LOV2, which the authors state makes it more suitable for use as an optogenetics tool.
YtvA is a homodimeric photo-switch, with a longer relaxation half-life than Avena sativa LOV2, making it more suitable for use as an optogenetics tool.
YtvA is a homodimeric photoswitch with a longer relaxation half-life than Avena sativa LOV2, which the authors state makes it more suitable for use as an optogenetics tool.
YtvA is a homodimeric photo-switch, with a longer relaxation half-life than Avena sativa LOV2, making it more suitable for use as an optogenetics tool.
YtvA is a homodimeric photoswitch with a longer relaxation half-life than Avena sativa LOV2, which the authors state makes it more suitable for use as an optogenetics tool.
YtvA is a homodimeric photo-switch, with a longer relaxation half-life than Avena sativa LOV2, making it more suitable for use as an optogenetics tool.
YtvA is a homodimeric photoswitch with a longer relaxation half-life than Avena sativa LOV2, which the authors state makes it more suitable for use as an optogenetics tool.
YtvA is a homodimeric photo-switch, with a longer relaxation half-life than Avena sativa LOV2, making it more suitable for use as an optogenetics tool.
YtvA is a homodimeric photoswitch with a longer relaxation half-life than Avena sativa LOV2, which the authors state makes it more suitable for use as an optogenetics tool.
YtvA is a homodimeric photo-switch, with a longer relaxation half-life than Avena sativa LOV2, making it more suitable for use as an optogenetics tool.
YtvA is a homodimeric photoswitch with a longer relaxation half-life than Avena sativa LOV2, which the authors state makes it more suitable for use as an optogenetics tool.
YtvA is a homodimeric photo-switch, with a longer relaxation half-life than Avena sativa LOV2, making it more suitable for use as an optogenetics tool.
YtvA is a homodimeric photoswitch with a longer relaxation half-life than Avena sativa LOV2, which the authors state makes it more suitable for use as an optogenetics tool.
YtvA is a homodimeric photo-switch, with a longer relaxation half-life than Avena sativa LOV2, making it more suitable for use as an optogenetics tool.
YtvA is a homodimeric photoswitch with a longer relaxation half-life than Avena sativa LOV2, which the authors state makes it more suitable for use as an optogenetics tool.
YtvA is a homodimeric photo-switch, with a longer relaxation half-life than Avena sativa LOV2, making it more suitable for use as an optogenetics tool.
YtvA is a homodimeric photoswitch with a longer relaxation half-life than Avena sativa LOV2, which the authors state makes it more suitable for use as an optogenetics tool.
YtvA is a homodimeric photo-switch, with a longer relaxation half-life than Avena sativa LOV2, making it more suitable for use as an optogenetics tool.
YtvA is a homodimeric photoswitch with a longer relaxation half-life than Avena sativa LOV2, which the authors state makes it more suitable for use as an optogenetics tool.
YtvA is a homodimeric photo-switch, with a longer relaxation half-life than Avena sativa LOV2, making it more suitable for use as an optogenetics tool.
YtvA is a homodimeric photoswitch with a longer relaxation half-life than Avena sativa LOV2, which the authors state makes it more suitable for use as an optogenetics tool.
YtvA is a homodimeric photo-switch, with a longer relaxation half-life than Avena sativa LOV2, making it more suitable for use as an optogenetics tool.
YtvA is a homodimeric photoswitch with a longer relaxation half-life than Avena sativa LOV2, which the authors state makes it more suitable for use as an optogenetics tool.
YtvA is a homodimeric photo-switch, with a longer relaxation half-life than Avena sativa LOV2, making it more suitable for use as an optogenetics tool.
YtvA is a homodimeric photoswitch with a longer relaxation half-life than Avena sativa LOV2, which the authors state makes it more suitable for use as an optogenetics tool.
YtvA is a homodimeric photo-switch, with a longer relaxation half-life than Avena sativa LOV2, making it more suitable for use as an optogenetics tool.
YtvA is a homodimeric photoswitch with a longer relaxation half-life than Avena sativa LOV2, which the authors state makes it more suitable for use as an optogenetics tool.
YtvA is a homodimeric photo-switch, with a longer relaxation half-life than Avena sativa LOV2, making it more suitable for use as an optogenetics tool.
YtvA is a homodimeric photoswitch with a longer relaxation half-life than Avena sativa LOV2, which the authors state makes it more suitable for use as an optogenetics tool.
YtvA is a homodimeric photo-switch, with a longer relaxation half-life than Avena sativa LOV2, making it more suitable for use as an optogenetics tool.
YtvA is a homodimeric photoswitch with a longer relaxation half-life than Avena sativa LOV2, which the authors state makes it more suitable for use as an optogenetics tool.
YtvA is a homodimeric photo-switch, with a longer relaxation half-life than Avena sativa LOV2, making it more suitable for use as an optogenetics tool.
YtvA is a homodimeric photoswitch with a longer relaxation half-life than Avena sativa LOV2, which the authors state makes it more suitable for use as an optogenetics tool.
YtvA is a homodimeric photo-switch, with a longer relaxation half-life than Avena sativa LOV2, making it more suitable for use as an optogenetics tool.
The YtvA mutant I113D is considered monomeric based on comparison against standards, but the abstract states that further experiments are needed to verify this.
Comparing against standards mutant I113D is considered as monomeric, however further experiments need to be conducted to verify this.
The YtvA mutant I113D is considered monomeric based on comparison against standards, but the abstract states that further experiments are needed to verify this.
Comparing against standards mutant I113D is considered as monomeric, however further experiments need to be conducted to verify this.
The YtvA mutant I113D is considered monomeric based on comparison against standards, but the abstract states that further experiments are needed to verify this.
Comparing against standards mutant I113D is considered as monomeric, however further experiments need to be conducted to verify this.
The YtvA mutant I113D is considered monomeric based on comparison against standards, but the abstract states that further experiments are needed to verify this.
Comparing against standards mutant I113D is considered as monomeric, however further experiments need to be conducted to verify this.
The YtvA mutant I113D is considered monomeric based on comparison against standards, but the abstract states that further experiments are needed to verify this.
Comparing against standards mutant I113D is considered as monomeric, however further experiments need to be conducted to verify this.
The YtvA mutant I113D is considered monomeric based on comparison against standards, but the abstract states that further experiments are needed to verify this.
Comparing against standards mutant I113D is considered as monomeric, however further experiments need to be conducted to verify this.
The YtvA mutant I113D is considered monomeric based on comparison against standards, but the abstract states that further experiments are needed to verify this.
Comparing against standards mutant I113D is considered as monomeric, however further experiments need to be conducted to verify this.
The YtvA mutant I113D is considered monomeric based on comparison against standards, but the abstract states that further experiments are needed to verify this.
Comparing against standards mutant I113D is considered as monomeric, however further experiments need to be conducted to verify this.
The YtvA mutant I113D is considered monomeric based on comparison against standards, but the abstract states that further experiments are needed to verify this.
Comparing against standards mutant I113D is considered as monomeric, however further experiments need to be conducted to verify this.
The YtvA mutant I113D is considered monomeric based on comparison against standards, but the abstract states that further experiments are needed to verify this.
Comparing against standards mutant I113D is considered as monomeric, however further experiments need to be conducted to verify this.
The YtvA mutant I113D is considered monomeric based on comparison against standards, but the abstract states that further experiments are needed to verify this.
Comparing against standards mutant I113D is considered as monomeric, however further experiments need to be conducted to verify this.
The YtvA mutant I113D is considered monomeric based on comparison against standards, but the abstract states that further experiments are needed to verify this.
Comparing against standards mutant I113D is considered as monomeric, however further experiments need to be conducted to verify this.
The YtvA mutant I113D is considered monomeric based on comparison against standards, but the abstract states that further experiments are needed to verify this.
Comparing against standards mutant I113D is considered as monomeric, however further experiments need to be conducted to verify this.
The YtvA mutant I113D is considered monomeric based on comparison against standards, but the abstract states that further experiments are needed to verify this.
Comparing against standards mutant I113D is considered as monomeric, however further experiments need to be conducted to verify this.
The YtvA mutant I113D is considered monomeric based on comparison against standards, but the abstract states that further experiments are needed to verify this.
Comparing against standards mutant I113D is considered as monomeric, however further experiments need to be conducted to verify this.
The YtvA mutant I113D is considered monomeric based on comparison against standards, but the abstract states that further experiments are needed to verify this.
Comparing against standards mutant I113D is considered as monomeric, however further experiments need to be conducted to verify this.
The YtvA mutant I113D is considered monomeric based on comparison against standards, but the abstract states that further experiments are needed to verify this.
Comparing against standards mutant I113D is considered as monomeric, however further experiments need to be conducted to verify this.
Approval Evidence
1 source2 linked approval claimsfirst-pass slug ytva-from-bacillus-subtilis
A second LOV domain, YtvA from Bacillus subtilis
Source:
comparative propertysupports
YtvA is a homodimeric photoswitch with a longer relaxation half-life than Avena sativa LOV2, which the authors state makes it more suitable for use as an optogenetics tool.
YtvA is a homodimeric photo-switch, with a longer relaxation half-life than Avena sativa LOV2, making it more suitable for use as an optogenetics tool.
Source:
structural statemixed
The YtvA mutant I113D is considered monomeric based on comparison against standards, but the abstract states that further experiments are needed to verify this.
Comparing against standards mutant I113D is considered as monomeric, however further experiments need to be conducted to verify this.
Source:
Comparisons
Source-backed strengths
The main reported strength is that YtvA is a homodimeric photoswitch with a longer relaxation half-life than Avena sativa LOV2. The source authors explicitly state that this feature makes it more suitable for use as an optogenetics tool.
Source:
YtvA is a homodimeric photo-switch, with a longer relaxation half-life than Avena sativa LOV2, making it more suitable for use as an optogenetics tool.
Compared with light-oxygen-voltage sensing (LOV) domain
YtvA from Bacillus subtilis and light-oxygen-voltage sensing (LOV) domain address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: heterodimerization; same primary input modality: light
Compared with optogenetic RGS2
YtvA from Bacillus subtilis and optogenetic RGS2 address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: heterodimerization; same primary input modality: light
Compared with split-TurboID
YtvA from Bacillus subtilis and split-TurboID address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: heterodimerization; same primary input modality: light
Relative tradeoffs: appears more independently replicated; looks easier to implement in practice.
Ranked Citations
- 1.