Toolkit Items

Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.

5 items matching 1 filter

Mechanism Branch

Layer 1

Mechanisms

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Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.

Layer 2

Architectures

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Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.

Layer 3

Components

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Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.

Technique Branch

Layer 1

Approaches

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High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.

Layer 2

Methods

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Concrete methods used to design, build, verify, or characterize engineered systems.

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anti-crispr-mediated inhibition

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SpCas9

Protein Domain
Since 2017anti-crispr-mediated inhibitiondna bindingDNA Bindingmicrorna-dependent post-transcriptional regulationPhotocleavage

SpCas9 is the Streptococcus pyogenes Cas9 CRISPR effector protein used for programmable genome editing and gene regulation. In the cited study, its activity was controlled indirectly by microRNA-dependent expression of the anti-CRISPR protein AcrIIA4, enabling cell-type-restricted activation of full-length Cas9, split-Cas9, and dCas9-VP64 variants.

CFBacMamMusHumTxRep
Ev 37Rep 20Pr 83

AcrIIC3-LOV2 light-switchable anti-CRISPR hybrid

Multi-Component Switch
Since 2019anti-crispr-mediated inhibitionlight-dependent allosteric switchingComputational DesignStructural Characterization

AcrIIC3-LOV2 is an engineered light-switchable anti-CRISPR protein formed by fusing the Neisseria meningitidis Cas9 inhibitor AcrIIC3 to the Avena sativa LOV2 blue-light sensory domain. In mammalian cells, two reported hybrids strongly inhibit NmeCas9 in the dark and allow robust genome editing under blue-light irradiation.

CFBacMamMusHumTxRep
Ev 44Rep 20Pr 49

CRISPR/Cas system

Multi-Component Switch
Since 2015anti-crispr-mediated inhibitionrna-guided genome editingselection/enrichment of edited mutantsFunctional AssaySelection / Enrichment

The CRISPR/Cas system is a multi-component genomic engineering platform composed of clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins. It is described as a versatile and powerful genetic tool for genome manipulation, with reported applicability across essentially any organism and cell type.

CFBacMamMusHumTxRep
Ev 20Rep 9Pr 49
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