Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
5 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-5 of 5
This delivery harness consists of viral vectors generated to express light-inducible recombinases in vivo. In the cited RecV system, Cre, Dre, and Flp were combined with the blue-light-responsive Vivid module to enable light-triggered genomic recombination in live mouse brain under one-photon or two-photon illumination.
FUN-LOVSP is a single-plasmid blue-light-responsive variant of the FUN-LOV optogenetic switch developed in yeast. It was generated by reconfiguring the original system with promoter and terminator replacements and supports light-induced reporter expression in selectable-marker derivatives including Nat- and Hph-marked formats.
FUN-LOVSP-Hph is a blue-light-responsive yeast optogenetic switch variant of FUN-LOVSP that includes a hygromycin resistance cassette for selection after genome integration. It drives light-induced reporter expression and was reported to outperform the original FUN-LOV system in BY4741 yeast while remaining functional in a wine yeast strain.
FUN-LOVSP-Nat is a blue-light-responsive yeast optogenetic switch variant derived from the FUN-LOVSP system and carrying a nourseothricin resistance marker. In BY4741 yeast, it supported blue-light-induced luciferase expression that exceeded the original FUN-LOV system in both episomal and genome-integrated formats.
The anchor review explicitly discusses OptoAMP as a high-light-sensitivity blue-light system for production settings, and the 2026 Biotechnol Adv review also covers it.