Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
7 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-7 of 7
Here, we describe an inducible and drug-selectable acoustic reporter gene system that can enable gas vesicle expression in mammalian cell lines, which we demonstrate using HEK293T cells.
The pharmaceutically controlled designer circuit is a synthetic multi-component signaling cascade activated by the clinically licensed antihypertensive drug guanabenz. In the reported system, guanabenz drives dose-dependent expression and secretion of a GLP-1-Fc(mIgG)-Leptin output relevant to metabolic syndrome treatment.
Chemical-controlled molecular switches are multi-component research tools in mammalian cell biology whose activity is regulated by chemical inducers. The supplied evidence establishes their general role as central instruments and identifies chemical input as the control modality, but does not specify particular switch architectures or target processes.
The copper-regulated expression system is described only at the level of a plant chemical-inducible transgene control strategy for regulating transcription. The supplied evidence supports that plant chemically inducible systems usually comprise a chimeric transcription factor and a reporter gene, but it does not define a copper-specific molecular implementation.
the Nature Communications article associated with DOI 10.1038/s41467-025-67201-w describes multi-layer chemically inducible CRISPR systems ... useful for extracting explicit component provenance and benchmark context for pTET:Ultra-tight
The steroid-regulated expression system is a plant chemical-inducible transcription control platform. The available evidence only supports that such systems typically include a chimeric transcription factor and a reporter gene, without describing a steroid-specific molecular architecture or performance.
The tetracycline-regulated expression system is a chemical-inducible transcriptional switch used in plants. The available evidence indicates that plant systems of this class usually comprise a chimeric transcription factor and a reporter gene.