hM3Ds
Construct PatternHere, we report the development of a whole sequence-humanized Gs-coupled DREADD, hM3Ds.
CFBacMamMusHumTxRep
Ev 32Rep 9Pr 71
Toolkit Items
Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
8 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
▾Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 1
Mechanisms
Layer 2
Architectures
▾Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 2
Architectures
Layer 3
Components
▾Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Layer 3
Components
Technique Branch
Layer 1
Approaches
▾High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 1
Approaches
Layer 2
Methods
▾Concrete methods used to design, build, verify, or characterize engineered systems.
Layer 2
Methods
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chemogenetic receptor activation
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rM3Ds
Construct PatternThe Gs-coupled DREADD, rM3Ds, modulates excitability in neuron subsets that are sensitive to downstream effectors of Gs protein.
CFBacMamMusHumTxRep
Ev 28Rep 9Pr 71
designer receptors exclusively activated by designer drug
Multi-Component SwitchDesigner receptors exclusively activated by designer drug (DREADDs) are engineered G protein-coupled receptors used as a chemogenetic or pharmacogenetic system. They enable selective remote control of neuronal activity through activation by otherwise inert drug-like small molecules.
CFBacMamMusHumTxRep
Ev 20Rep 9Pr 37
designer receptors exclusively activated by designer drugs
Multi-Component Switchchemogenetic receptor activationg protein-coupled signaling via gq and gi designer receptorsreversible neuromodulationComputational Design
We highlight the following technologies: ... designer receptors exclusively activated by designer drugs.
CFBacMamMusHumTxRep
DREADDs
Multi-Component Switchcell-type-specific activation or silencing of neuronal firingchemogenetic receptor activationgpcr signaling modulationComputational Design
Chemogenetic tools allow the expression of designer receptors exclusively activated by designer drugs (DREADDs) specifically in microglia.
CFBacMamMusHumTxRep
hM3D(Gq)
Multi-Component SwitchUnexpectedly, in response to the administration of deschloroclozapine, we observed inhibitory effects with pan-neuronal hM3D(Gq) stimulation.
CFBacMamMusHumTxRep
hM3Dq DREADD
Multi-Component SwitchWe injected adeno-associated virus vectors expressing the excitatory DREADD hM3Dq into the unilateral substantia nigra (SN) in four marmosets.
CFBacMamMusHumTxRep
The sequential activation of hM3D(Gq) and KORD DREADDs within the same neuronal population validated the role of the VTA-AcbSh circuit in reinforced responding for concurrently available natural and drug rewards.
CFBacMamMusHumTxRep
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