Toolkit Items

Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.

4 items matching 1 filter

Mechanism Branch

Layer 1

Mechanisms

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Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.

Layer 2

Architectures

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Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.

Layer 3

Components

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Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.

Technique Branch

Layer 1

Approaches

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High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.

Layer 2

Methods

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Concrete methods used to design, build, verify, or characterize engineered systems.

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co-expression-based functional complementation

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ChR2(C128A)

Protein Domain

Since 2009co-expression-based functional complementation light-gated ion channel activation step-function photoswitching

ChR2(C128A) is a C128A step-function mutant of channelrhodopsin-2 used as a light-responsive protein domain for prolonged optogenetic activation. The supplied evidence indicates that it is unsuitable for chronic neuronal stimulation but is useful for light-controlled induction of immediate early genes, including c-fos promoter-driven protein expression with precise timing and single-cell specificity.

CFBacMamMusHumTxRep

Ev 37Rep 20Pr 71

SSFO

Protein Domain

Since 2022co-expression-based functional complementation optogenetic ion channel activation step-function photoswitching

SSFO is a stabilized step-function opsin used as an optogenetic actuator for neuronal control. In a 2022 theoretical study, SSFO was evaluated in co-expression with the fast channelrhodopsin ChETA to support sustained optical switching and improved high-frequency spiking fidelity at low power.

CFBacMamMusHumTxRep

Ev 28Rep 9Pr 71

ChR2(C128S)

Protein Domain

Since 2022co-expression-based functional complementation light-gated ion channel activation step-function photoswitching

ChR2(C128S) is a step-function channelrhodopsin variant identified as one of several step-function opsins considered in a 2022 theoretical optogenetics study. In that study, it is modeled in co-expression with the fast channelrhodopsin ChETA to support sustained neuronal switching and improved spiking performance.

CFBacMamMusHumTxRep

Ev 28Rep 9Pr 59

ChETA

Protein Domain

Since 2022co-expression-based functional complementation light-gated ion channel activity

ChETA is a light-responsive protein domain considered here only in a theoretical co-expression strategy with step-function opsins (SFOs). In that context, modeling predicted that ChETA-SFO co-expression in hippocampal neurons would stabilize photocurrent and reduce spike failure associated with photocurrent desensitization.

CFBacMamMusHumTxRep

Ev 10Rep 9Pr 59

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