Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
2 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-2 of 2
The Boolean logic gate is a construct pattern incorporated into a photoactivatable CRISPR/Cas9-inducible DNAzyme probe. In the reported system, it contributed to superior spatiotemporal control for dynamic imaging of nuclear Zn2+ in HeLa cells and mice.
CRISPR-DNAzyme is a three-stranded DNAzyme probe engineered for in situ imaging of nuclear Zn2+ in living cells. Its catalytic activity is initially blocked by a 20-bp CRISPR/Cas9 recognition site and is activated in the nucleus after Cas9/sgRNA cleavage forms the active DNAzyme structure.