Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
2 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
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The GUS-NC80 fusion protein is a transgenic plant construct in which β-glucuronidase is fused to the 80-residue NC80 motif from Arabidopsis CRY2. In the cited study, this fusion was constitutively active despite being unphosphorylated, supporting that NC80 is sufficient to confer CRY2 physiological function when derepressed.
The CRY2 C-terminal tail from Arabidopsis CRY2 contains an 80-residue NC80 motif that is sufficient to confer CRY2 physiological function. Evidence from transgenic plant studies indicates that this region participates in blue light-responsive regulation through phosphorylation-linked derepression of NC80.