Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
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Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
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Cu-catalyzed azide-alkyne cycloaddition (CuAAC) is a click-chemistry method used to cyclize RNA through covalent intramolecular ligation. In a 2024 study on cyclic photocleavable RNA for a photoactivatable CRISPR/Cas9 system, CuAAC was tested alongside thiol-maleimide Michael reaction and was reported to have advantages for cyclic RNA preparation.