Toolkit Items

Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.

2 items matching 1 filter

Mechanism Branch

Layer 1

Mechanisms

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Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.

Layer 2

Architectures

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Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.

Layer 3

Components

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Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.

Technique Branch

Layer 1

Approaches

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High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.

Layer 2

Methods

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Concrete methods used to design, build, verify, or characterize engineered systems.

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covalent ligand immobilization

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Gelatin-linked synNotch ligand hydrogel platform

Construct Pattern
Since 2023covalent ligand immobilizationmaterial-to-cell signalingsynthetic notch receptor activationtranscriptional program induction

The gelatin-linked synNotch ligand hydrogel platform is a material-to-cell signaling system in which synNotch ligands are covalently attached to gelatin polymers by enzymatic conjugation or click chemistry. These ligand-functionalized hydrogels activate synthetic Notch receptors in mammalian cells cultured on or within the material, enabling prescribed transcriptional responses and spatial control of cell phenotypes.

CFBacMamMusHumTxRep
Ev 28Rep 9Pr 71

Material-to-cell synNotch ligand platforms

Delivery Strategy
Since 2023covalent ligand immobilizationmaterial-presented ligand recognitionsynthetic notch receptor activationsynthetic receptor activationtranscriptional program induction

Material-to-cell synNotch ligand platforms are engineered biomaterial and extracellular matrix systems that present synNotch ligands to mammalian cells. In the reported 2023 implementation, ligands were covalently incorporated into gelatin hydrogels or into cell-generated fibronectin-containing extracellular matrix to activate synthetic Notch receptors and induce programmed transcriptional outputs.

CFBacMamMusHumTxRep
Ev 28Rep 9Pr 71
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