BG-Cy3-Cy5
Protein DomainSpecifically, we synthesized a probe that contains Cy3 and Cy5 dye molecules as well as a BG moiety (Scheme 1). The BG permits the targeting of the Cy3-Cy5 photoswitch to SNAP-tag fusion proteins.
CFBacMamMusHumTxRep
Toolkit Items
Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
2 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
▾Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 1
Mechanisms
Layer 2
Architectures
▾Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 2
Architectures
Layer 3
Components
▾Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Layer 3
Components
Technique Branch
Layer 1
Approaches
▾High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 1
Approaches
Layer 2
Methods
▾Concrete methods used to design, build, verify, or characterize engineered systems.
Layer 2
Methods
Sort
covalent snap-tag labeling
Showing 1-2 of 2
Loaditems
Page 1 / 1
β-tubulin-SNAP
Protein DomainTowards this end, we expressed β-tubulin as a C-terminal fusion of SNAP-tag (β-tubulin-SNAP) in U2OS cells.
CFBacMamMusHumTxRep
Page 1 / 1