Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
7 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
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Ultimately, we generated 12 independent Cas9-free, glycoengineered, homozygous lines. This work establishes the first N. benthamiana lines that are fully Cas9-free and homozygously edited at all seven key glycosyltransferase loci.
CRY1 knockout cells are human U-2 OS osteosarcoma cell models in which the CRY1 locus was disrupted using duplex CRISPR/Cas9 editing. The reported approach uses two guide RNAs targeting intronic regions flanking a CRY exon to remove whole exons and generate a human circadian clock knockout model.
CRY2 knockout cells are human U-2 OS osteosarcoma cell models in which the CRY2 gene was disrupted using duplex CRISPR/Cas9. They were generated by deleting whole CRY exons with two guide RNAs targeting exon-flanking intronic regions and display long-period circadian phenotypes consistent with CRY loss.
Whole-genome screening of gene knockout mutants in Toxoplasma gondii is a CRISPR/Cas9-based assay method for generating and interrogating genome-scale loss-of-function mutant populations. The cited review identifies this as a recent use of CRISPR/Cas9 in an apicomplexan parasite.
CRY1/CRY2 double knockout cells are human U-2 OS osteosarcoma cell models in which both cryptochrome genes were disrupted using duplex CRISPR/Cas9. The tool was generated by deleting whole exons with paired guide RNAs targeting exon-flanking intronic regions, producing a double-knockout circadian phenotype reported as arrhythmic.
To assist users in CRISPR/Cas9-based genome engineering, we developed CrisprBuildr, an open-source, web-based application for designing modifications to their target genes. CrisprBuildr guides users through creating guide RNAs and repair template vectors to generate cloning maps.
silicene-Cas9 is a NIR-II light-controlled CRISPR/Cas9 nanosystem built on biosafe, biodegradable 2D silicene nanosheets with photothermal-conversion capability. It is reported to mediate remote light-triggered TXNDC5 gene knockdown and to couple genome editing with tumor microenvironment reprogramming and augmented photonic tumor ablation.