Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
3 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-3 of 3
A light-activated CRISPR activation system is a construct pattern reported for spatiotemporal control of gene expression in zebrafish (Danio rerio). The available evidence indicates that light is used as the input to regulate CRISPR-based transcriptional activation in vivo.
FUS-CRISPR(a/i) is a focused-ultrasound-controllable CRISPR toolbox comprising CRISPRa, CRISPRi, and CRISPR systems that incorporate heat-sensitive genetic modules. It is designed to regulate genome and epigenome functions in live cells and animals with spatiotemporal control.
ChemHEAL is a small-molecule-inducible variant of the HEAL dCas12f-based CRISPR activation platform for programmable transcriptional control. It uses a multi-component architecture in which transcriptional activators are recruited through MS2 coat protein binding to MS2 aptamers embedded in the sgRNA scaffold.