Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
5 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-5 of 5
Photoactivatable CRISPR-Cas9 is an optogenetic genome-editing construct pattern in which Cas9-mediated gene perturbation is controlled by light. It has been applied in mice to induce LIF knockdown under light-emitting diode illumination, enabling spatiotemporal perturbation of embryo implantation-associated biology and fertility.
The CRISPR-Cas9 piggyBac system is a regulatable genome engineering delivery harness reported in human cells. It combines CRISPR-Cas9 with a piggyBac-based system to enable site-specific randomization of the endogenous genome.
Using a transfection-infection assay (T-I assay), the AcMNPV gp64 gene was targeted at six different locations to evaluate the effects of single and multiple targeting sites
This tool is an adeno-associated virus-mediated CRISPR/Cas9 genome editing system in which guide RNA expression is placed under doxycycline control. The inducible AAV vector expresses a gRNA from an H1/TO promoter together with TetR, enabling doxycycline-dependent genome editing in vitro and in vivo, including in mouse brain.
We then used CRISPR-Cas9 to engineer the P. aeruginosa phage PaGZ-1 to express these biofilm-disrupting genes. The resulting engineered phages demonstrated superior inhibition of biofilm formation compared to the wild-type phage.