PACE (Phage Assisted Continuous Evolution) is an engineering method used in this study to evolve cryptochrome properties. In the cited work, it was applied to increase the dynamic range of the blue-light-dependent interaction between Arabidopsis thaliana CRY2 and BIC1.
Toolkit Items
Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
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Mechanism Branch
Layer 1
Mechanisms
▾Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 1
Mechanisms
Layer 2
Architectures
▾Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 2
Architectures
Layer 3
Components
▾Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Layer 3
Components
Technique Branch
Layer 1
Approaches
▾High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 1
Approaches
Layer 2
Methods
▾Concrete methods used to design, build, verify, or characterize engineered systems.
Layer 2
Methods
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