Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
10 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-10 of 10
Domain fusion is a protein engineering method in which protein domains are fused or split to improve existing protein functions or create novel functions. In the supplied evidence, it is described as a general strategy for expanding CRISPR-Cas9 applications.
Protein splitting is a protein engineering method in which proteins are modified through domain fusion or splitting to improve existing functions or develop novel functions. In the provided evidence, it is discussed as a strategy relevant to expanding CRISPR-Cas9 applications.
The Avena sativa LOV2 blue light sensory domain is a photosensory protein module used in engineered AcrIIC3-LOV2 fusion proteins to confer blue-light control over Neisseria meningitidis Cas9 activity. In this reported context, LOV2 enabled dark-state inhibition and light-permissive genome editing in mammalian cells.
VP16-EL222 is a chimeric blue light-responsive transcription factor composed of the photosensitive protein EL222 fused to the transcriptional activator VP16. In Yarrowia lipolytica, it functions with a promoter containing (C120)5 and the minimal promoter CYC102 to drive blue light-induced GFPMut3 expression.
Hybrid protein optogenetics is an engineering method for introducing light regulation into proteins by fusing a protein of interest to photoreactive biological modules. It is presented in a 2022 review as one of three major strategies for targeted photocontrol of protein function.
Synthetic biology approaches for opto-protein development are protein engineering strategies for creating and optimizing non-neuronal light-regulatable proteins. The cited review describes modifying photosensitive domains and fusing them to effector domains to generate light-controllable protein functions.
Recent advances have extended these synthetic scaffolds to active RNA editors by fusing them to catalytically competent DYW deaminase domains, generating customizable enzymes capable of precise base conversion in bacteria, plants, and even human cells.
The nanopore electrophoretic driver protein-LOV2 fusion is a protein-domain fusion in which a nanopore electrophoretic driver protein is attached to the LOV2 domain. In the cited Chemical Science study, this fusion enhanced capture efficiency of the target protein in a solid-state nanopore single-molecule detection context.
In addition, we describe more recently developed tools such as opsin/G protein-coupled receptor chimeric molecules
Additionally, TadA8e-nIscB exhibits limited activity. However, fusing an extra copy of TadA-8e to either terminus of TadA8e-nIsc significantly enhances A-to-G conversions.