Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
2 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-2 of 2
The optimized CRISPR-Cas9-based light-inducible gene expression device is a multi-component light-on transcription system built on CRISPR-Cas9-based engineering. It was reported to control gene transcription in a dose-dependent manner and was applied to induce P53 expression in bladder cancer cells.
The pharmaceutically controlled designer circuit is a synthetic multi-component signaling cascade activated by the clinically licensed antihypertensive drug guanabenz. In the reported system, guanabenz drives dose-dependent expression and secretion of a GLP-1-Fc(mIgG)-Leptin output relevant to metabolic syndrome treatment.