Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
5 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-5 of 5
dCas9-based gene networks are a construct pattern used in combination with a synthetic demultiplexer to build pulsatile-signal filters and decoders within synthetic dynamic signal-processing circuits. In the cited 2021 study, these networks contributed to decoding complex temporal inputs into differential gene expression outputs.
The demultiplexer for dynamically encoded signals is a synthetic gene circuit architecture that decodes temporally encoded inputs into distinct gene-expression outputs. In a 2021 study, this circuit was shown to demultiplex dynamic signals and was further combined with dCas9-based gene networks to build pulsatile-signal filters and decoders.
Dynamic multiplexing is a computational design principle for synthetic gene networks that encodes and decodes time-varying inputs into distinct gene expression states. In the cited 2021 study, it increased information transmission from signal to gene expression and enabled dynamic signal decoding using engineered regulators with different response kinetics.
Pulsatile-signal filters and decoders are synthetic gene-network constructs generated by combining a demultiplexer with dCas9-based regulatory networks. They are designed to decode complex dynamic input patterns, including pulsatile signals, into differential gene-expression outputs.
The falling-edge pulse-detector is a synthetic light-input transcriptional circuit built from light-responsive transcriptional regulators with differing response kinetics. It is designed to decode temporal features of illumination and specifically detect the falling edge of a light pulse.