Toolkit Items

Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.

11 items matching 1 filter

Mechanism Branch

Layer 1

Mechanisms

Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.

Layer 2

Architectures

Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.

Layer 3

Components

Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.

Technique Branch

Layer 1

Approaches

High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.

Layer 2

Methods

Concrete methods used to design, build, verify, or characterize engineered systems.

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feedback control

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feed-forward and feedback control technique

Engineering Method

The feed-forward and feedback control technique is an engineering method proposed for astrocytes that manipulates intracellular IP3 to stabilize Ca2+ concentration. It is described in the context of Ca2+-based molecular communications nanonetworks, where controlled Ca2+ dynamics are intended to support more reliable signaling behavior.

CFBacMamMusHumTxRep
Ev 28Rep 9Pr 71

closed-loop gene networks

Construct Pattern

Closed-loop gene networks are synthetic mammalian gene circuit architectures assembled from gene switches in engineered cells. Reported functions of these complex circuits include event memory, oscillatory protein production, and information-processing behaviors, and microencapsulated mammalian cells carrying such networks have been implanted into mice.

CFBacMamMusHumTxRep
Ev 20Rep 9Pr 71

automated optogenetic feedback control

Engineering Method

Automated optogenetic feedback control is an engineering method that combines light-driven optogenetic regulation with a feedback loop to control gene expression and cell growth. The available evidence supports it as a control framework for precise and robust regulation rather than as a single molecular reagent.

CFBacMamMusHumTxRep
Ev 28Rep 9Pr 59

optoPlateReader

Delivery Strategy

The optoPlateReader (oPR) is an open-source, solid-state, compact hardware platform for automated optogenetic stimulation and spectroscopy in each well of a 96-well plate. It combines an optoPlate illumination module with an optoReader module containing 96 photodiodes and LEDs to enable 96 parallel light measurements and feedback-enabled optical experiments.

CFBacMamMusHumTxRep
Ev 28Rep 9Pr 59

bioreactor designs for optogenetic stimulation

Delivery Strategy

Bioreactor designs for optogenetic stimulation are light-delivery and culture-platform configurations used to stimulate optogenetic systems across experimental formats ranging from simple illumination set-ups to microscopy, microtiter plate, and bioreactor designs. These platforms support applications spanning single-cell stimulation to whole-culture illumination and can be integrated with automated measurement and stimulation for computer-controlled experiments.

CFBacMamMusHumTxRep
Ev 20Rep 9Pr 59

in silico feedback control strategies

Engineering Method

In silico feedback control strategies are computationally implemented control schemes coupled to optogenetic measurement and light stimulation platforms. They are used to create computer-controlled living systems through automated measurement and stimulation workflows.

CFBacMamMusHumTxRep
Ev 20Rep 9Pr 59

light-induction hardware-software platforms

Delivery Strategy

Light-induction hardware-software platforms are optogenetic delivery systems that provide controlled illumination using formats ranging from simple illumination set-ups to microscopy, microtiter plate, and bioreactor designs. They are used to stimulate biological systems with light, and automated implementations can support computer-controlled experiments with in silico feedback control.

CFBacMamMusHumTxRep
Ev 20Rep 9Pr 59

microtiter plate designs for optogenetic stimulation

Delivery Strategy

Microtiter plate designs for optogenetic stimulation are light-delivery platform formats used to apply regulated optical inputs in biological experiments. The cited review places these designs within a broader range of optogenetic hardware spanning simple illumination setups to microtiter plate and bioreactor implementations, with use cases from single-cell stimulation to whole-culture illumination.

CFBacMamMusHumTxRep
Ev 20Rep 9Pr 59
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