Toolkit Items

Light-sheet microscopy, also termed single plane illumination microscopy, is an in vivo fluorescence imaging method tailored to larval research and embryonic imaging. The supplied evidence indicates that it can capture the full course of embryonic development from egg to larva and has been coupled with optogenetic perturbation to study Wnt signaling during embryogenesis.

Lipid uptake and localization was studied by flow cytometry and confocal laser scanning microscopy (CLSM) and revealed a rapid uptake by bacteria within 5 min.

Optical imaging methods covered in this review include fluorescence imaging in the near-infrared (NIR) region...

Here, we review the recent literature dealing with the design and application of QD-bioconjugates for advanced in vitro and in vivo imaging.

Whole-retina-to-brain imaging was performed using fMOST on samples injected with the optimal condition (AAV2.2-double-1/1000), enabling the reconstruction of complete axonal trajectories of individual PV+ RGCs from the retina to the brain.

We have developed a novel approach for high-resolution confocal imaging across multiple tissue planes simultaneously. By combining confocal microscopy, piezo actuators, and optogenetic sensors, we can simultaneously capture images of dynamic fluorescence signals from various cell populations in different tissue layers (Z planes).

Laser scanning confocal microscopy was used to determine the location of virus entrapment within the virus filtration membrane.

recent applications and reasonable expectations from current light-sheet fluorescence microscopy (LSFM) and super-resolution microscopy (SRM) modalities

Head mounted fluorescent microscopes are light-based imaging tools used in systems neuroscience to image neurons, neurocircuits, and their inputs and outputs. The supplied evidence places them within the recent expansion of functional imaging approaches but does not specify a particular instrument architecture or readout.

Remaining challenges include brightness/photostability limits and the need for broader translational validation, yet progress in structure-guided mutagenesis, computational/AI-assisted protein design, and hybrid imaging strategies promises to close these gaps.

Today, intravital microscopy with two-photon excitation microscopes (2P-IVM) is the mainstay technique for observing intercellular cross-talks in situ, unraveling cellular and molecular mechanisms in the context of their spatiotemporal dynamics.

The ability to simultaneously image the spatiotemporal activity signatures from many neurons during unrestrained vertebrate behaviors has become possible through the development of miniaturized fluorescence microscopes, or miniscopes, sufficiently light to be carried by small animals such as bats, birds and rodents.

Of particular importance is the polymer encapsulated nanoparticles containing ... fluorogens with aggregation induced emission (AIE) characteristics as the core, which have shown significant advantages in terms of tunable brightness, superb photo- and physical stability, good biocompatibility, potential biodegradability and facile surface functionalization.