Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
4 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-4 of 4
Fluorescence resonance energy transfer-fluorescence lifetime imaging microscopy (FRET-FLIM) is an assay method that combines fluorescence resonance energy transfer with fluorescence lifetime imaging to detect molecular proximity in living cells. In the cited Arabidopsis study, it was used to support a physical interaction between CRY2 and SPA1 in nuclei.
TR-FRET assay is a time-resolved fluorescence resonance energy transfer binding assay used in the cited study to confirm binding of a small-molecule ligand to CIB1. In this evidence set, it functions as a chemical-input assay for ligand-binding confirmation.
Here, we review a broad spectrum of single-molecule tools and techniques such as ... single-molecule fluorescence resonance energy transfer (smFRET)...
Methods like FRET, chemical probing and even the humble polyacrylamide gel can be rapid and very powerful, allowing the examination of a large number of sequence variants relatively quickly.