fluorescence line narrowing
Assay MethodFluorescence line narrowing (FLN) is a spectroscopic assay method used in the cited study to interrogate the electronic structure of flavin mononucleotide (FMN) within phototropin LOV2 domains. In this context, FLN was applied to support mechanistic analysis of how the conserved cysteine near FMN perturbs the chromophore ground state and promotes photochemistry.