Toolkit Items

Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.

2 items matching 1 filter

Mechanism Branch

Layer 1

Mechanisms

Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.

Layer 2

Architectures

Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.

Layer 3

Components

Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.

Technique Branch

Layer 1

Approaches

High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.

Layer 2

Methods

Concrete methods used to design, build, verify, or characterize engineered systems.

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fluorescence spectroscopy

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fluorescence line narrowing

Assay Method

Fluorescence line narrowing (FLN) is a spectroscopic assay method used in the cited study to interrogate the electronic structure of flavin mononucleotide (FMN) within phototropin LOV2 domains. In this context, FLN was applied to support mechanistic analysis of how the conserved cysteine near FMN perturbs the chromophore ground state and promotes photochemistry.

CFBacMamMusHumTxRep
Ev 28Rep 9Pr 71
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