Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
7 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-7 of 7
we engineered six-helix bundle DNA origami-CRISPR complexes that achieved nucleolin-targeted genome editing in cervical cancer cells, coupling GFP-based diagnostics with therapeutic E7 oncogene disruption
The bicistronic expression cassette with GFP is a construct pattern used in Caenorhabditis elegans to co-express GFP as a reporter alongside conditional Channelrhodopsin-2 targeting components. In the cited study, GFP was included to identify the correct expression pattern during recombinase-based, intersectional single-neuron targeting.
In a recent study, we developed a novel fluorescent biosensor to directly visualize OMM rupture. This technique enables temporal and spatial characterization of OMM rupture and provides a powerful platform to dissect the underlying mechanism.
In our most optimal conditions, up to 4.83 ×10 11 /ml of HEK293 EVs were successfully cell-free engineered with a fusion membrane protein incorporating CD63 I-shaped membrane-insertion topology transmembrane helix 3 (CD63ITM3) and monomeric green lantern (mGL).
Elucidation of the spatiotemporal nature of the hormone network can be accomplished by using genetically engineered biosensors.
These reporters support real-time tracking of infection dynamics and host-virus interactions and power diagnostic platforms including reporter viruses, CRISPR-based assays, and nanotechnology-enhanced biosensors.
We also discuss the potential application of red-shifted indicators along with optogenetic tools to 2P-IVM.