Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
5 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-5 of 5
LIGHT knockout by homologous recombination is a mouse gene-targeting method used to generate LIGHT-/- mice. In the cited study, these knockout mice were used to test how LIGHT deficiency influences cardiac allograft survival and responses to cyclosporine A.
CRY2 knockout cells are human U-2 OS osteosarcoma cell models in which the CRY2 gene was disrupted using duplex CRISPR/Cas9. They were generated by deleting whole CRY exons with two guide RNAs targeting exon-flanking intronic regions and display long-period circadian phenotypes consistent with CRY loss.
Cib1(-/-) mice are a constitutive Mus musculus knockout line generated by homologous recombination in embryonic stem cells to disrupt the Cib1 gene. This in vivo genetic perturbation was used to define CIB1 function and revealed that CIB1 is essential for male spermatogenesis.
Whole-genome screening of gene knockout mutants in Toxoplasma gondii is a CRISPR/Cas9-based assay method for generating and interrogating genome-scale loss-of-function mutant populations. The cited review identifies this as a recent use of CRISPR/Cas9 in an apicomplexan parasite.
Countermeasures now integrate ... CRISPR/Cas9-validated receptor knockouts revealing functional redundancy