Toolkit Items

Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.

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Mechanism Branch

Layer 1

Mechanisms

Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.

Layer 2

Architectures

Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.

Layer 3

Components

Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.

Technique Branch

Layer 1

Approaches

High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.

Layer 2

Methods

Concrete methods used to design, build, verify, or characterize engineered systems.

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genetic overexpression

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HY5ox/pifQein3eil1

Multi-Component Switch

HY5ox/pifQein3eil1 is an Arabidopsis multi-component genetic switch-like genotype comprising HY5 overexpression combined with loss of EIN3, EIL1, and four PIF genes. In dark-grown seedlings, this combination drives strong photomorphogenic development and produces phenotypes highly similar to wild-type seedlings grown in continuous light.

CFBacMamMusHumTxRep
Ev 28Rep 9Pr 37
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