Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
5 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-5 of 5
LIGHT knockout by homologous recombination is a mouse gene-targeting method used to generate LIGHT-/- mice. In the cited study, these knockout mice were used to test how LIGHT deficiency influences cardiac allograft survival and responses to cyclosporine A.
Gene replacement by homologous recombination in Physcomitrella patens is a targeted genome engineering method for plant genome modification. The cited evidence states that this moss supports gene replacement at high frequency through homologous recombination.
By using a homology-integrated CRISPR RNA array, we utilized the multiplexing capability of CRISPR/Cas12a to realize multiplex genetic variant installation
Cib1(-/-) mice are a constitutive Mus musculus knockout line generated by homologous recombination in embryonic stem cells to disrupt the Cib1 gene. This in vivo genetic perturbation was used to define CIB1 function and revealed that CIB1 is essential for male spermatogenesis.
we established an efficient homology-integrated CRISPR/Cas12a system to install genetic variants through homologous recombination by targeting T-rich PAMs