Toolkit Items

Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.

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Mechanism Branch

Layer 1

Mechanisms

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Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.

Layer 2

Architectures

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Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.

Layer 3

Components

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Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.

Technique Branch

Layer 1

Approaches

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High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.

Layer 2

Methods

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Concrete methods used to design, build, verify, or characterize engineered systems.

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induced subcellular translocation

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blue light-activated PKC isozyme recruitment system

Multi-Component Switch
Since 2022Heterodimerizationinduced subcellular translocationlight-induced heterodimerizationproximity-driven membrane protein phosphorylation

The blue light-activated PKC isozyme recruitment system is a multi-component optogenetic switch that uses the plant-derived CRY2-CIB1 interaction to recruit PKC isozyme catalytic domains to the cell surface under blue light. In the reported format, CRY2-tagged PKC catalytic domains undergo light-triggered membrane translocation, and a PKCε implementation robustly activates GIRK1/4 potassium channels.

CFBacMamMusHumTxRep
Ev 28Rep 9Pr 37
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