Toolkit Items

Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.

2 items matching 1 filter

Mechanism Branch

Layer 1

Mechanisms

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Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.

Layer 2

Architectures

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Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.

Layer 3

Components

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Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.

Technique Branch

Layer 1

Approaches

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High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.

Layer 2

Methods

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Concrete methods used to design, build, verify, or characterize engineered systems.

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jα-helix unfolding

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Jalpha helix point mutations disrupting LOV-domain interaction

Construct Pattern
Since 2004constitutive kinase activationDegradationdisruption of lov-jalpha intramolecular interactiondisruption of lov–jα intramolecular interactionjalpha helix displacementjalpha helix unfoldingjα-helix unfolding

Jalpha helix point mutations disrupting LOV-domain interaction are a phototropin construct pattern in which residues along the Jα helix are mutated to weaken LOV–Jα intramolecular association. In full-length phototropin, these mutations displace or unfold the Jα helix and produce constitutive kinase activation in the absence of illumination.

CFBacMamMusHumTxRep
Ev 28Rep 9Pr 59

Zdk2-AsLOV2 optogenetic construct

Protein Domain
Since 2020blue-light-induced cysteinyl-fmn adduct formationconformational uncagingConformational Uncagingjα-helix unfoldingStructural Characterization

Zdk2-AsLOV2 is an optogenetic protein-domain construct built around the Avena sativa LOV2 photosensory module. The supplied evidence supports blue-light activation through canonical AsLOV2 photochemistry, including cysteinyl-FMN adduct formation and Jα-helix unfolding, but does not describe the specific functional role contributed by the Zdk2 fusion in cells.

CFBacMamMusHumTxRep
Ev 28Rep 9Pr 59
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